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Literature summary extracted from

  • Musdal, Y.; Mannervik, B.
    Substrate specificities of two tau class glutathione transferases inducible by 2,4,6-trinitrotoluene in poplar (2015), Biochim. Biophys. Acta, 1850, 1877-1883.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expressed in Escherichia coli BL21 Gold (DE3) cells Populus trichocarpa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.0065
-
 phenethyl isothiocyanate isoform GSTU16, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 0.0256
-
 sulforaphane isoform GSTU45, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 0.036
-
 phenethyl isothiocyanate isoform GSTU45, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 0.038
-
 cumene hydroperoxide isoform GSTU16, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 0.29
-
 1-chloro-2,4-dinitrobenzene isoform GSTU45, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 0.324
-
 allyl-isothiocyanate isoform GSTU45, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 0.759
-
 allyl-isothiocyanate isoform GSTU16, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 1.69
-
 1-chloro-2,4-dinitrobenzene isoform GSTU16, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 4.853
-
 cumene hydroperoxide isoform GSTU45, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 6.178
-
 sulforaphane isoform GSTU16, pH not specified in the publication, at 30°C Populus trichocarpa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 25378
-
-
 Populus trichocarpa
2.5.1.18 25521
-
-
 Populus trichocarpa

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Populus trichocarpa D2WL48
-
-
2.5.1.18 Populus trichocarpa D2WL57
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 Ni-Sepharose column chromatography Populus trichocarpa
2.5.1.18 Ni-Sepharose column chromatography, and gel filtration Populus trichocarpa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione
-
 Populus trichocarpa S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione isoform GSTU45 shows highest activity with 1-chloro-2,4-dinitrobenzene Populus trichocarpa S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole + glutathione
-
 Populus trichocarpa ?
-
 ?
2.5.1.18 allyl-isothiocyanate + glutathione
-
 Populus trichocarpa ?
-
 ?
2.5.1.18 benzyl-isothiocyanate + glutathione
-
 Populus trichocarpa ?
-
 ?
2.5.1.18 cumene hydroperoxide + glutathione
-
 Populus trichocarpa ?
-
 ?
2.5.1.18 additional information very low or no activity with iodomethane, 1-iodobutane, 1-iodooctane, H2O2, dehydroascorbate, trans-2-hexenal, trans-2-nonenal, 2,4,6-trinitrotoluene, and 2,4-dinitrotoluene Populus trichocarpa ?
-
 ?
2.5.1.18 phenethyl isothiocyanate + glutathione
-
 Populus trichocarpa ?
-
 ?
2.5.1.18 phenethyl isothiocyanate + glutathione isoform GSTU16 shows highest activity with phenethyl isothiocyanate Populus trichocarpa ?
-
 ?
2.5.1.18 propyl-isothiocyanate + glutathione
-
 Populus trichocarpa ?
-
 ?
2.5.1.18 sulforaphane + glutathione
-
 Populus trichocarpa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 ? x * 25378, isoform GSTU45, calculated from amino acid sequence Populus trichocarpa
2.5.1.18 ? x * 25521, isoform GSTU16, calculated from amino acid sequence Populus trichocarpa

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GSTU16 isoform Populus trichocarpa
2.5.1.18 GSTU45 isoform Populus trichocarpa
2.5.1.18 tau class glutathione transferase
-
 Populus trichocarpa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 0.073
-
 sulforaphane isoform GSTU45, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 0.14
-
 phenethyl isothiocyanate isoform GSTU45, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 0.25
-
 allyl-isothiocyanate isoform GSTU45, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 0.27
-
 cumene hydroperoxide isoform GSTU45, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 0.58
-
 cumene hydroperoxide isoform GSTU16, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 2.57
-
 sulforaphane isoform GSTU16, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 2.89
-
 1-chloro-2,4-dinitrobenzene isoform GSTU16, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 3.6
-
 allyl-isothiocyanate isoform GSTU16, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 5.3
-
 1-chloro-2,4-dinitrobenzene isoform GSTU45, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 62.9
-
 phenethyl isothiocyanate isoform GSTU16, pH not specified in the publication, at 30°C Populus trichocarpa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.18 Populus trichocarpa isoform GSTU16, calculated from amino acid sequence
-
 5.6
2.5.1.18 Populus trichocarpa isoform GSTU45, calculated from amino acid sequence
-
 6

Expression

EC Number Organism Comment Expression
2.5.1.18 Populus trichocarpa expression is induced by exposure of poplar tissues to 2,4,6-trinitrotoluene up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.18 0.06
-
 cumene hydroperoxide isoform GSTU45, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 0.42
-
 sulforaphane isoform GSTU16, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 0.77
-
 allyl-isothiocyanate isoform GSTU45, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 1.7
-
 1-chloro-2,4-dinitrobenzene isoform GSTU16, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 2.84
-
 sulforaphane isoform GSTU45, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 3.75
-
 phenethyl isothiocyanate isoform GSTU45, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 4.74
-
 allyl-isothiocyanate isoform GSTU16, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 15.37
-
 cumene hydroperoxide isoform GSTU16, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 18.31
-
 1-chloro-2,4-dinitrobenzene isoform GSTU45, pH not specified in the publication, at 30°C Populus trichocarpa
2.5.1.18 9696
-
 phenethyl isothiocyanate isoform GSTU16, pH not specified in the publication, at 30°C Populus trichocarpa