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Literature summary extracted from

  • Allen, K.D.; Wang, S.C.
    Spectroscopic characterization and mechanistic investigation of P-methyl transfer by a radical SAM enzyme from the marine bacterium Shewanella denitrificans OS217 (2014), Biochim. Biophys. Acta, 1844, 2135-2144.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.326 SD_1168 from Shewanella denitrificans is cloned into pET-30a(+)for protein overexpression. SD_1168 is found exclusively in inclusion bodies when overexpressed in either Escherichia coli or Streptomyces lividans. All efforts to express soluble recombinant protein are unsuccessful. Therefore SD_1168 is refolded using an established refolding procedure Shewanella denitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.326 [4Fe-4S] cluster the enzyme binds 6.1 mol of iron per mol protein and 4.0 mol of sulfid Shewanella denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.326 64800
-
x * 64800, SDS-PAGE Shewanella denitrificans

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.326 Shewanella denitrificans
-
-
-
2.1.1.326 Shewanella denitrificans OS217
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.326 2 S-adenosyl-L-methionine + demethylphosphinothricin + reduced acceptor
-
Shewanella denitrificans S-adenosyl-L-homocysteine + 5'-deoxyadenosine + L-methionine + phosphinothricin + oxidized acceptor
-
?
2.1.1.326 2 S-adenosyl-L-methionine + demethylphosphinothricin + reduced acceptor
-
Shewanella denitrificans OS217 S-adenosyl-L-homocysteine + 5'-deoxyadenosine + L-methionine + phosphinothricin + oxidized acceptor
-
?
2.1.1.326 2 S-adenosyl-L-methionine + N-acetyldemethylphosphinothricin + reduced acceptor
-
Shewanella denitrificans S-adenosyl-L-homocysteine + 5'-deoxyadenosine + L-methionine + N-acetylphosphinothricin + oxidized acceptor
-
?
2.1.1.326 2 S-adenosyl-L-methionine + N-acetyldemethylphosphinothricin + reduced acceptor
-
Shewanella denitrificans OS217 S-adenosyl-L-homocysteine + 5'-deoxyadenosine + L-methionine + N-acetylphosphinothricin + oxidized acceptor
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.326 ? x * 64800, SDS-PAGE Shewanella denitrificans

Synonyms

EC Number Synonyms Comment Organism
2.1.1.326 SD_1168
-
Shewanella denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.326 methylcob(III)alamin the enzyme catalyzes P-methylation using radical S-adenosyl-L-methionine-dependent chemistry with cobalamin as a coenzymemethyl donor of the reaction. The enzyme is active in the absence of methylcobalamin if the strong reductant titanium(III)citrate and hydroxocobalamin are provided Shewanella denitrificans