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Literature summary extracted from

  • Graczer, E.; Szimler, T.; Garamszegi, A.; Konarev, P.V.; Labas, A.; Olah, J.; Pallo, A.; Svergun, D.I.; Merli, A.; Zavodszky, P.; Weiss, M.S.; Vas, M.
    Dual role of the active site residues of Thermus thermophilus 3-isopropylmalate dehydrogenase: chemical catalysis and domain closure (2016), Biochemistry, 55, 560-574.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.85 D217A the mutant retains 1.1% of the catalytic activity of the wild type enzyme Thermus thermophilus
1.1.1.85 D241A the mutant shows a drastic decrease in the kcat value (0.06% compared to that of the wild type enzyme) Thermus thermophilus
1.1.1.85 D245A the mutant retains 10.5% of the catalytic activity of the wild type enzyme Thermus thermophilus
1.1.1.85 E270A the mutation leads to a significant increase in the Km value for NAD+ compared to the wild type enzyme Thermus thermophilus
1.1.1.85 K185A the mutant shows a drastic decrease in the kcat value (0.06% compared to that of the wild type enzyme) Thermus thermophilus
1.1.1.85 N102A the mutant retains 13% of the catalytic activity of the wild type enzyme Thermus thermophilus
1.1.1.85 Y139A the mutant retains 2.9% of the catalytic activity of the wild type enzyme Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.85 0.013
-
 (2R,3S)-3-isopropylmalate mutant enzyme D217A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.014
-
 (2R,3S)-3-isopropylmalate mutant enzyme N102A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.016
-
 (2R,3S)-3-isopropylmalate wild type enzyme, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.02
-
 (2R,3S)-3-isopropylmalate mutant enzyme D245A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.032
-
 (2R,3S)-3-isopropylmalate mutant enzyme E270A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.04
-
 (2R,3S)-3-isopropylmalate mutant enzyme Y139A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.06
-
 NAD+ mutant enzyme Y139A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.064
-
 NAD+ mutant enzyme K185A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.145
-
 (2R,3S)-3-isopropylmalate mutant enzyme K185A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.255
-
 (2R,3S)-3-isopropylmalate mutant enzyme D241A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.26
-
 NAD+ mutant enzyme N102A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.29
-
 NAD+ wild type enzyme, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.305
-
 NAD+ mutant enzyme D217A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.32
-
 NAD+ mutant enzyme D241A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.375
-
 NAD+ mutant enzyme D245A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.66
-
 NAD+ mutant enzyme E270A, at pH 7.6 and 20°C Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.85 Mn2+ required Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ Thermus thermophilus
-
 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Thermus thermophilus Q5SIY4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Thermus thermophilus (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.85 IPMDH
-
 Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.85 0.0025
-
 (2R,3S)-3-isopropylmalate mutant enzyme D241A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.0025
-
 (2R,3S)-3-isopropylmalate mutant enzyme K185A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.033
-
 (2R,3S)-3-isopropylmalate mutant enzyme E270A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.045
-
 (2R,3S)-3-isopropylmalate mutant enzyme D217A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.108
-
 (2R,3S)-3-isopropylmalate mutant enzyme Y139A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.433
-
 (2R,3S)-3-isopropylmalate mutant enzyme D245A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 0.55
-
 (2R,3S)-3-isopropylmalate mutant enzyme N102A, at pH 7.6 and 20°C Thermus thermophilus
1.1.1.85 40
-
 (2R,3S)-3-isopropylmalate wild type enzyme, at pH 7.6 and 20°C Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.85 NAD+
-
 Thermus thermophilus