BRENDA - Enzyme Database

Structural Basis for iron-mediated sulfur transfer in archael and yeast thiazole synthases

Zhang, X.; Eser, B.E.; Chanani, P.K.; Begley, T.P.; Ealick, S.E.; Biochemistry 55, 1826-1838 (2016) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.8.1.10
expressed in Escherichia coli BL21(DE3) cells
Methanocaldococcus jannaschii
2.8.1.10
expressed in Escherichia coli BL21(DE3) cells
Saccharomyces cerevisiae
2.8.1.10
expressed in Escherichia coli BL21(DE3) cells
Methanotorris igneus
Crystallization (Commentary)
EC Number
Crystallization (Commentary)
Organism
2.8.1.10
C205S variant with a bound glycine imine intermediate, hanging drop vapor diffusion method, using 25% (w/v) PEG1500, 0.0125 M succinic acid, 0.044 M sodium dihydrogen phosphate, and 0.044 M glycine, pH 8.5
Saccharomyces cerevisiae
2.8.1.10
wild type enzyme complexed with ADP-ribulose, hanging drop vapor diffusion method, using 1.05 M potassium/sodium tartrate, 0.1 M CHES/sodium hydroxide, pH 9.5 and 0.2 M lithium sulfate
Methanocaldococcus jannaschii
2.8.1.10
wild type enzyme with bound glycine imine intermediate and iron, hanging drop vapor diffusion method, using 1.0 M sodium citrate, 0.1 M CHES/sodium hydroxide, pH 9.5
Methanotorris igneus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.8.1.10
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
Methanocaldococcus jannaschii
-
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
?
2.8.1.10
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
Saccharomyces cerevisiae
-
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
?
2.8.1.10
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
Methanotorris igneus
-
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.8.1.10
Methanocaldococcus jannaschii
-
-
-
2.8.1.10
Methanotorris igneus
-
-
-
2.8.1.10
Saccharomyces cerevisiae
P32318
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.8.1.10
Ni-NTA column chromatography and Superdex 200 gel filtration
Methanocaldococcus jannaschii
2.8.1.10
Ni-NTA column chromatography and Superdex 200 gel filtration
Saccharomyces cerevisiae
2.8.1.10
Ni-NTA column chromatography and Superdex 200 gel filtration
Methanotorris igneus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.8.1.10
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
737721
Methanocaldococcus jannaschii
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
-
?
2.8.1.10
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
737721
Saccharomyces cerevisiae
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
-
?
2.8.1.10
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
737721
Methanotorris igneus
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.8.1.10
Thi4
-
Methanocaldococcus jannaschii
2.8.1.10
Thi4
-
Saccharomyces cerevisiae
2.8.1.10
Thi4
-
Methanotorris igneus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.1.10
expressed in Escherichia coli BL21(DE3) cells
Methanocaldococcus jannaschii
2.8.1.10
expressed in Escherichia coli BL21(DE3) cells
Saccharomyces cerevisiae
2.8.1.10
expressed in Escherichia coli BL21(DE3) cells
Methanotorris igneus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.8.1.10
C205S variant with a bound glycine imine intermediate, hanging drop vapor diffusion method, using 25% (w/v) PEG1500, 0.0125 M succinic acid, 0.044 M sodium dihydrogen phosphate, and 0.044 M glycine, pH 8.5
Saccharomyces cerevisiae
2.8.1.10
wild type enzyme complexed with ADP-ribulose, hanging drop vapor diffusion method, using 1.05 M potassium/sodium tartrate, 0.1 M CHES/sodium hydroxide, pH 9.5 and 0.2 M lithium sulfate
Methanocaldococcus jannaschii
2.8.1.10
wild type enzyme with bound glycine imine intermediate and iron, hanging drop vapor diffusion method, using 1.0 M sodium citrate, 0.1 M CHES/sodium hydroxide, pH 9.5
Methanotorris igneus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.8.1.10
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
Methanocaldococcus jannaschii
-
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
?
2.8.1.10
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
Saccharomyces cerevisiae
-
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
?
2.8.1.10
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
Methanotorris igneus
-
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.1.10
Ni-NTA column chromatography and Superdex 200 gel filtration
Methanocaldococcus jannaschii
2.8.1.10
Ni-NTA column chromatography and Superdex 200 gel filtration
Saccharomyces cerevisiae
2.8.1.10
Ni-NTA column chromatography and Superdex 200 gel filtration
Methanotorris igneus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.8.1.10
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
737721
Methanocaldococcus jannaschii
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
-
?
2.8.1.10
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
737721
Saccharomyces cerevisiae
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
-
?
2.8.1.10
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
737721
Methanotorris igneus
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
-
?