BRENDA - Enzyme Database

Characterization of Lipoyl Synthase from Mycobacterium tuberculosis

Lanz, N.D.; Lee, K.H.; Horstmann, A.K.; Pandelia, M.E.; Cicchillo, R.M.; Krebs, C.; Booker, S.J.; Biochemistry 55, 1372-1383 (2016)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.1.8
protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] ferredoxin
Mycobacterium tuberculosis
-
protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] ferredoxin
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.1.8
Mycobacterium tuberculosis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.8.1.8
Ni-NTA resin chromatography and Sephacryl 200 gel filtration
Mycobacterium tuberculosis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.1.8
H protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] dithionite
-
737720
Mycobacterium tuberculosis
H protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] dithionite
-
-
-
?
2.8.1.8
H protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] flavodoxin
-
737720
Mycobacterium tuberculosis
H protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] flavodoxin
-
-
-
?
2.8.1.8
protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] ferredoxin
-
737720
Mycobacterium tuberculosis
protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] ferredoxin
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.8.1.8
4Fe-4S-center
the enzyme LipA harbors two [4Fe-4S] clusters that are essential for its activity
Mycobacterium tuberculosis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.8.1.8
4Fe-4S-center
the enzyme LipA harbors two [4Fe-4S] clusters that are essential for its activity
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.1.8
protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] ferredoxin
Mycobacterium tuberculosis
-
protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] ferredoxin
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.1.8
Ni-NTA resin chromatography and Sephacryl 200 gel filtration
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.1.8
H protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] dithionite
-
737720
Mycobacterium tuberculosis
H protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] dithionite
-
-
-
?
2.8.1.8
H protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] flavodoxin
-
737720
Mycobacterium tuberculosis
H protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] flavodoxin
-
-
-
?
2.8.1.8
protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [2Fe-2S] ferredoxin
-
737720
Mycobacterium tuberculosis
protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [2Fe-2S] ferredoxin
-
-
-
?