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Literature summary extracted from

  • Kumar, A.; Arya, R.; Makwana, P.K.; Dangi, R.S.; Yadav, U.; Surolia, A.; Kundu, S.; Sundd, M.
    The structure of the holo-acyl carrier protein of Leishmania major displays a remarkably different phosphopantetheinyl transferase binding interface (2015), Biochemistry, 54, 5632-5645.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.7 gene BSU03570, recombinant expression of His-tagged enzyme from Bacillus subtilis in Escherichia coli Bacillus subtilis
2.7.8.7 gene LMJF_20_0830, recombinant expression of His-tagged enzyme from Leishmania major in Escherichia coli Leishmania major

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.7 EDTA complete inhibition at 50 mM Bacillus subtilis
2.7.8.7 EDTA complete inhibition at 50 mM Leishmania major

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.7 additional information
-
 additional information quantitative kinetic measurements, steady-state kinetics Bacillus subtilis
2.7.8.7 0.0038
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, mutant F44M ACP substrate from Leishmania major Bacillus subtilis
2.7.8.7 0.0048
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/Q48E ACP substrate from Leishmania major Bacillus subtilis
2.7.8.7 0.00524
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/F44M ACP substrate from Leishmania major Bacillus subtilis
2.7.8.7 0.0055
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/F44M/Q48E ACP substrate from Leishmania major Bacillus subtilis
2.7.8.7 0.0063
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, wild-type ACP substrate from Leishmania major Bacillus subtilis
2.7.8.7 0.0269
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D ACP substrate from Leishmania major Bacillus subtilis
2.7.8.7 0.101
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, ACP substrate from Mycobacterium tuberculosis Bacillus subtilis
2.7.8.7 0.12
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, Escherichia coli ACP substrate Bacillus subtilis
2.7.8.7 0.13
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, Escherichia coli mutant M44F ACP substrate Bacillus subtilis
2.7.8.7 0.215
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, ACP substrate from Plasmodium falciparum Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.7 Mg2+ required Bacillus subtilis
2.7.8.7 Mg2+ required Leishmania major

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] Bacillus subtilis
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] Leishmania major
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] Bacillus subtilis 168
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Bacillus subtilis P39135
-
-
2.7.8.7 Bacillus subtilis 168 P39135
-
-
2.7.8.7 Leishmania major Q4QCW3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.7 recombinant His-tagged enzyme from Bacillus subtilis from Escherichia coli Bacillus subtilis
2.7.8.7 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Leishmania major

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
 Bacillus subtilis adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
 Leishmania major adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] assay with ACP substrates from Leishmania major (LmACP), Escherichia coli, Plasmodium falciparum, Mycobacterium tuberculosis, and Homo sapiens. The structure of the holo-acyl carrier protein of Leishmania major is similar to other type II ACPs, comprising a four-helix bundle, enclosing a hydrophobic core, but it displays a remarkably different phosphopantetheinyl transferase binding interface. Two- and three-dimensional NMR structure analysis Leishmania major adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
 Bacillus subtilis 168 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] assay with wild-type and mutant ACP substrates from Leishmania major (LmACP), mutants N35D, F44M, and F44A, the double mutants N35D/F44M and N35D/Q48E, triple mutant N35D/F44M/Q48E of LmACP, and with Escherichia coli ACP, the M44F mutant of Escherichia coli ACP, Plasmmodium falciparum ACP, and Mycobacterium tuberculosis ACP. No activity with LmACP F44A mutant Bacillus subtilis ?
-
 ?
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] assay with wild-type and mutant ACP substrates from Leishmania major (LmACP), mutants N35D, F44M, and F44A, the double mutants N35D/F44M and N35D/Q48E, triple mutant N35D/F44M/Q48E of LmACP, and with Escherichia coli ACP, the M44F mutant of Escherichia coli ACP, Plasmmodium falciparum ACP, and Mycobacterium tuberculosis ACP. No activity with LmACP F44A mutant Bacillus subtilis 168 ?
-
 ?
2.7.8.7 additional information recombinant expression of ACP substrates from Leishmania major (LmACP), Escherichia coli, Plasmodium falciparum, Mycobacterium tuberculosis, and Homo sapiens in Escherichia coli and purification by ion exchange chromatography. LmACP does not interact with the bacterial group I, 4'-phosphopantetheinyl transferase. Residues Asn 35 and Phe 44, present in LmACP modulate its interaction with AcpS Leishmania major ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.7 4'-phosphopantetheinyl transferase
-
 Bacillus subtilis
2.7.8.7 4'-phosphopantetheinyl transferase
-
 Leishmania major
2.7.8.7 BcPPT
-
 Bacillus subtilis
2.7.8.7 BSU03570
-
 Bacillus subtilis
2.7.8.7 LMJF_20_0830
-
 Leishmania major
2.7.8.7 LmPPT
-
 Leishmania major
2.7.8.7 phosphopantetheinyl transferase
-
 Bacillus subtilis
2.7.8.7 phosphopantetheinyl transferase
-
 Leishmania major
2.7.8.7 Sfp
-
 Bacillus subtilis
2.7.8.7 sfp lpa-8
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.7 37
-
 assay at Bacillus subtilis
2.7.8.7 37
-
 assay at Leishmania major

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.8.7 0.00017
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, mutant F44M ACP substrate from Leishmania major Bacillus subtilis
2.7.8.7 0.00083
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, wild-type ACP substrate from Leishmania major Bacillus subtilis
2.7.8.7 0.001
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/F44M/Q48E ACP substrate from Leishmania major Bacillus subtilis
2.7.8.7 0.0012
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/F44M ACP substrate from Leishmania major Bacillus subtilis
2.7.8.7 0.0017
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/Q48E ACP substrate from Leishmania major Bacillus subtilis
2.7.8.7 0.0038
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D ACP substrate from Leishmania major Bacillus subtilis
2.7.8.7 0.0082
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, Escherichia coli mutant M44F ACP substrate Bacillus subtilis
2.7.8.7 0.0103
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, ACP substrate from Mycobacterium tuberculosis Bacillus subtilis
2.7.8.7 0.014
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, Escherichia coli ACP substrate Bacillus subtilis
2.7.8.7 0.0192
-
 apo-[acyl-carrier protein] pH 8.0, 37°C, recombinant His-tagged enzyme, ACP substrate from Plasmodium falciparum Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.7 8
-
 assay at Bacillus subtilis
2.7.8.7 8
-
 assay at Leishmania major