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Literature summary extracted from

  • Pallares, I.G.; Moore, T.C.; Escalante-Semerena, J.C.; Brunold, T.C.
    Spectroscopic studies of the Salmonella enterica adenosyltransferase enzyme SeCobA: molecular-level insight into the mechanism of substrate Cob(II)alamin activation (2014), Biochemistry, 53, 7969-7982.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.17 expressed in Escherichia coli BL21 cells Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.17 F91W with cob(II)alamin the mutant shows catalytic activity diminished relative to that of wild type enzyme, while the activity with cob(I)alamin is largely retained Salmonella enterica
2.5.1.17 F91W/W93F in this mutant the yield of 4c cob(II)alamin species is reduced more than 10fold from that achieved by the wild type enzyme Salmonella enterica
2.5.1.17 F91Y with cob(II)alamin the mutant shows catalytic activity 5fold increased relative to that of wild type enzyme Salmonella enterica
2.5.1.17 W93A the mutation completely abolishes the catalytic activity with cob(II)alamin while modest activity is retained with cob(I)alamin Salmonella enterica
2.5.1.17 W93F the mutant shows wild type activity Salmonella enterica
2.5.1.17 W93H the mutation inhibits the formation of 4c cob(II)alamin Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.17 0.0163
-
 cob(II)inamide wild type enzyme, pH and temperature not specified in the publication Salmonella enterica
2.5.1.17 0.0254
-
 ATP wild type enzyme, pH and temperature not specified in the publication Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.17 ATP + cob(I)yrinic acid a,c-diamide Salmonella enterica
-
 triphosphate + adenosylcob(III)yrinic acid a,c-diamide
-
 ?
2.5.1.17 ATP + cobinamide Salmonella enterica
-
 triphosphate + adenosylcobinamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.17 Salmonella enterica P31570 serovar typhimurium LT2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.17 HisTrap nickel affinity column chromatography Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.17 ATP + cob(I)alamine
-
 Salmonella enterica triphosphate + adenosylcob(I)alamine
-
 ?
2.5.1.17 ATP + cob(I)yrinic acid a,c-diamide
-
 Salmonella enterica triphosphate + adenosylcob(III)yrinic acid a,c-diamide
-
 ?
2.5.1.17 ATP + cob(II)alamine
-
 Salmonella enterica triphosphate + adenosylcob(II)alamine
-
 ?
2.5.1.17 ATP + cob(II)inamide
-
 Salmonella enterica triphosphate + adenosylcob(II)inamide
-
 ?
2.5.1.17 ATP + cobinamide
-
 Salmonella enterica triphosphate + adenosylcobinamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.17 ACAT
-
 Salmonella enterica
2.5.1.17 ATP:co(I)rrinoid adenosyltransferase
-
 Salmonella enterica
2.5.1.17 CobA
-
 Salmonella enterica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.17 0.0067
-
 ATP wild type enzyme, pH and temperature not specified in the publication Salmonella enterica
2.5.1.17 0.0077
-
 cob(II)inamide wild type enzyme, pH and temperature not specified in the publication Salmonella enterica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.17 0.26
-
 ATP wild type enzyme, pH and temperature not specified in the publication Salmonella enterica
2.5.1.17 0.47
-
 cob(II)inamide wild type enzyme, pH and temperature not specified in the publication Salmonella enterica