BRENDA - Enzyme Database

Distinct properties of the two isoforms of CDP-diacylglycerol synthase

DSouza, K.; Kim, Y.J.; Balla, T.; Epand, R.M.; Biochemistry 53, 7358-7367 (2014)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
GTP
small stimulation; small stimulation
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.7.41
expression in COS-7 cell; expression in COS-7 cell; recombinant expression of C-terminally myc-tagged and GFP-tagged CDS1 in COS7 cells; recombinant expression of C-terminally myc-tagged and GFP-tagged CDS2 in COS7 cells
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
L-alpha-phosphatidylinositol 4,5-bisphosphate
1 mol%, 80% inhibition; strongest inhibitor among the phosphatidylinositols tested
Homo sapiens
2.7.7.41
L-alpha-phosphatidylinositol 4-phosphate
1 mol%, 50% inhibition
Homo sapiens
2.7.7.41
additional information
no significant inhibition in activity when either soybean or liver phosphatidylinositol is used at equimolar concentrations with respect to substrate. Neither soy nor egg phosphatidylglycerol has any significant inhibitory effect when used at equimolar concentrations with respect to substrate. Isoform CDS1 shows no acyl chain-dependent inhibition for 1-stearoyl-2-arachidonoyl-snphosphatidylinositol, 1-stearoyl-2-linoleoyl-sn-phosphatidylinositol, or 1,2-diolinoleoyl-sn-phosphatidylinositol
Homo sapiens
2.7.7.41
phosphatidylinositol
15-20% inhibition by soybean and liver phosphatidylinositol, inhibition of CDS2 by phosphatidylinositol is acyl chain-dependent, with the strongest inhibition seen with the 1-stearoyl-2-arachidonoyl species; CDS1 shows no acyl chain-dependent inhibition; significant inhibition of isoform CDS2 by both soybean and liver PI, with 15?20% residual activity
Homo sapiens
2.7.7.41
phosphatidylinositol 4-phosphate
; 55% inhibition at 1 mol%
Homo sapiens
2.7.7.41
phosphatidylinositol-(4,5)-bisphosphate
80% inhibition at 1 mol%, CDS2 is inhibited by its anionic phospholipid end products, with phosphatidylinositol-(4,5)-bisphosphate showing the strongest inhibition; CDS1 is inhibited by their anionic phospholipid end products, with phosphatidylinositol-(4,5)-bisphosphate showing the strongest inhibition
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
additional information
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
Km value 0.8 mol%, pH 8.0, 22°C; Km value 1.4 mol%, pH 8.0, 22°C
Homo sapiens
2.7.7.41
additional information
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
KM value 0.6 mol%, pH 8.0, 22°C; KM value 0.9 mol%, pH 8.0, 22°C
Homo sapiens
2.7.7.41
additional information
-
additional information
0.8 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.6 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview; 1.4 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.9 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
endoplasmic reticulum
ER tubular localization; ER tubular localization; GFP-tagged CDS1 shows a typical ER tubular localization when it is expressed in COS7 cells; GFP-tagged CDS2 shows a typical ER tubular localization when it is expressed in COS7 cells
Homo sapiens
5783
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
Mg2+
required; required
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.7.41
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
Homo sapiens
-
diphosphate + CDP-1-stearoyl-2-arachidonoyl-glycerol
-
-
?
2.7.7.41
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
Homo sapiens
-
diphosphate + CDP-1-stearoyl-2-linoleoyl-glycerol
-
-
-
2.7.7.41
CTP + phosphatidate
Homo sapiens
isozyme CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested
diphosphate + CDP-diacylglycerol
-
-
?
2.7.7.41
CTP + phosphatidate
Homo sapiens
isozyme CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
diphosphate + CDP-diacylglycerol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.41
Homo sapiens
O95674
-
-
2.7.7.41
Homo sapiens
Q92903
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.41
2.4
-
substrate 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, pH 8.0, 22°C
Homo sapiens
2.7.7.41
4.5
-
substrate 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, pH 8.0, 22°C
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + 1,2-diarachidonoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1,2-diarachidonoylglycerol
-
-
-
?
2.7.7.41
CTP + 1,2-diarachidonoyl-sn-phosphatidic acid
50% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1,2-diarachidonoylglycerol
-
-
-
?
2.7.7.41
CTP + 1,2-dilinoleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1,2-dilinoleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1,2-dilinoleoyl-sn-phosphatidic acid
20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1,2-dilinoleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1,2-dioleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1,2-dioleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1,2-dioleoyl-sn-phosphatidic acid
less than 5% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1,2-dioleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1,2-diolinoleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1,2-diolinoleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-palmitoyl-2-arachidonoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-palmitoyl-2-arachidonoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-palmitoyl-2-arachidonoyl-sn-phosphatidic acid
65% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1-palmitoyl-2-arachidonoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-arachidonoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-arachidonoyl-glycerol
-
-
-
?
2.7.7.41
CTP + 1-stearoyl-2-docosahexaenoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-docosahexaenoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-linoleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-linoleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-linoleoyl-glycerol
-
-
-
-
2.7.7.41
CTP + 1-stearoyl-2-oleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-oleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-stearoyl-2-oleoyl-sn-phosphatidic acid
20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-oleoylglycerol
25% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
-
?
2.7.7.41
CTP + phosphatidate
-
737701
Homo sapiens
diphosphate + CDP-diacylglycerol
-
-
-
?
2.7.7.41
CTP + phosphatidate
isozyme CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested
737701
Homo sapiens
diphosphate + CDP-diacylglycerol
-
-
-
?
2.7.7.41
CTP + phosphatidate
isozyme CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-diacylglycerol
-
-
-
?
2.7.7.41
additional information
CDS2 shows substrate specificity at both the sn-1 and sn-2 acyl chain positions. The most preferred substrate is 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
?
-
-
-
-
2.7.7.41
additional information
isoform CDS1 appears to have no substrate preference for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, with variations of the sn-1 and sn-2 acyl chains resulting in no significant changes in preference
737701
Homo sapiens
?
-
-
-
-
2.7.7.41
additional information
mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS1, overview
737701
Homo sapiens
?
-
-
-
-
2.7.7.41
additional information
mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS2, overview
737701
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.7.41
22
-
assay at room temperature; assay at room temperature
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.7.41
2.9
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
pH 8.0, 22°C; pH 8.0, 22°C, recombinant CDS1
Homo sapiens
2.7.7.41
2.9
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
pH 8.0, 22°C; pH 8.0, 22°C, recombinant CDS2
Homo sapiens
2.7.7.41
3.2
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
pH 8.0, 22°C; pH 8.0, 22°C, recombinant CDS1
Homo sapiens
2.7.7.41
8
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
pH 8.0, 22°C; pH 8.0, 22°C, recombinant CDS2
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
8
-
assay at; assay at
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.7.41
GTP
small stimulation
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.41
expression in COS-7 cell; recombinant expression of C-terminally myc-tagged and GFP-tagged CDS1 in COS7 cells
Homo sapiens
2.7.7.41
expression in COS-7 cell; recombinant expression of C-terminally myc-tagged and GFP-tagged CDS2 in COS7 cells
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.41
L-alpha-phosphatidylinositol 4,5-bisphosphate
strongest inhibitor among the phosphatidylinositols tested
Homo sapiens
2.7.7.41
L-alpha-phosphatidylinositol 4,5-bisphosphate
1 mol%, 80% inhibition
Homo sapiens
2.7.7.41
L-alpha-phosphatidylinositol 4-phosphate
1 mol%, 50% inhibition
Homo sapiens
2.7.7.41
additional information
no significant inhibition in activity when either soybean or liver phosphatidylinositol is used at equimolar concentrations with respect to substrate. Neither soy nor egg phosphatidylglycerol has any significant inhibitory effect when used at equimolar concentrations with respect to substrate. Isoform CDS1 shows no acyl chain-dependent inhibition for 1-stearoyl-2-arachidonoyl-snphosphatidylinositol, 1-stearoyl-2-linoleoyl-sn-phosphatidylinositol, or 1,2-diolinoleoyl-sn-phosphatidylinositol
Homo sapiens
2.7.7.41
phosphatidylinositol
CDS1 shows no acyl chain-dependent inhibition
Homo sapiens
2.7.7.41
phosphatidylinositol
15-20% inhibition by soybean and liver phosphatidylinositol, inhibition of CDS2 by phosphatidylinositol is acyl chain-dependent, with the strongest inhibition seen with the 1-stearoyl-2-arachidonoyl species; significant inhibition of isoform CDS2 by both soybean and liver PI, with 15?20% residual activity
Homo sapiens
2.7.7.41
phosphatidylinositol 4-phosphate
-
Homo sapiens
2.7.7.41
phosphatidylinositol 4-phosphate
55% inhibition at 1 mol%
Homo sapiens
2.7.7.41
phosphatidylinositol-(4,5)-bisphosphate
CDS1 is inhibited by their anionic phospholipid end products, with phosphatidylinositol-(4,5)-bisphosphate showing the strongest inhibition
Homo sapiens
2.7.7.41
phosphatidylinositol-(4,5)-bisphosphate
80% inhibition at 1 mol%, CDS2 is inhibited by its anionic phospholipid end products, with phosphatidylinositol-(4,5)-bisphosphate showing the strongest inhibition
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.41
additional information
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
Km value 0.8 mol%, pH 8.0, 22°C
Homo sapiens
2.7.7.41
additional information
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
Km value 1.4 mol%, pH 8.0, 22°C
Homo sapiens
2.7.7.41
additional information
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
KM value 0.6 mol%, pH 8.0, 22°C
Homo sapiens
2.7.7.41
additional information
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
KM value 0.9 mol%, pH 8.0, 22°C
Homo sapiens
2.7.7.41
additional information
-
additional information
0.8 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.6 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview
Homo sapiens
2.7.7.41
additional information
-
additional information
1.4 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.9 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.7.41
endoplasmic reticulum
ER tubular localization; GFP-tagged CDS1 shows a typical ER tubular localization when it is expressed in COS7 cells
Homo sapiens
5783
-
2.7.7.41
endoplasmic reticulum
ER tubular localization; GFP-tagged CDS2 shows a typical ER tubular localization when it is expressed in COS7 cells
Homo sapiens
5783
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.41
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.7.41
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
Homo sapiens
-
diphosphate + CDP-1-stearoyl-2-arachidonoyl-glycerol
-
-
?
2.7.7.41
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
Homo sapiens
-
diphosphate + CDP-1-stearoyl-2-linoleoyl-glycerol
-
-
-
2.7.7.41
CTP + phosphatidate
Homo sapiens
isozyme CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested
diphosphate + CDP-diacylglycerol
-
-
?
2.7.7.41
CTP + phosphatidate
Homo sapiens
isozyme CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
diphosphate + CDP-diacylglycerol
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.41
2.4
-
substrate 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, pH 8.0, 22°C
Homo sapiens
2.7.7.41
4.5
-
substrate 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, pH 8.0, 22°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.41
CTP + 1,2-diarachidonoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1,2-diarachidonoylglycerol
-
-
-
?
2.7.7.41
CTP + 1,2-diarachidonoyl-sn-phosphatidic acid
50% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1,2-diarachidonoylglycerol
-
-
-
?
2.7.7.41
CTP + 1,2-dilinoleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1,2-dilinoleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1,2-dilinoleoyl-sn-phosphatidic acid
20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1,2-dilinoleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1,2-dioleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1,2-dioleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1,2-dioleoyl-sn-phosphatidic acid
less than 5% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1,2-dioleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1,2-diolinoleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1,2-diolinoleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-palmitoyl-2-arachidonoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-palmitoyl-2-arachidonoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-palmitoyl-2-arachidonoyl-sn-phosphatidic acid
65% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1-palmitoyl-2-arachidonoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-arachidonoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-arachidonoyl-glycerol
-
-
-
?
2.7.7.41
CTP + 1-stearoyl-2-docosahexaenoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-docosahexaenoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-linoleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-linoleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-linoleoyl-glycerol
-
-
-
-
2.7.7.41
CTP + 1-stearoyl-2-oleoyl-sn-phosphatidic acid
-
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-oleoylglycerol
-
-
-
?
2.7.7.41
CTP + 1-stearoyl-2-oleoyl-sn-phosphatidic acid
20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-1-stearoyl-2-oleoylglycerol
25% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
-
?
2.7.7.41
CTP + phosphatidate
-
737701
Homo sapiens
diphosphate + CDP-diacylglycerol
-
-
-
?
2.7.7.41
CTP + phosphatidate
isozyme CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested
737701
Homo sapiens
diphosphate + CDP-diacylglycerol
-
-
-
?
2.7.7.41
CTP + phosphatidate
isozyme CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
diphosphate + CDP-diacylglycerol
-
-
-
?
2.7.7.41
additional information
CDS2 shows substrate specificity at both the sn-1 and sn-2 acyl chain positions. The most preferred substrate is 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
737701
Homo sapiens
?
-
-
-
-
2.7.7.41
additional information
isoform CDS1 appears to have no substrate preference for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, with variations of the sn-1 and sn-2 acyl chains resulting in no significant changes in preference
737701
Homo sapiens
?
-
-
-
-
2.7.7.41
additional information
mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS1, overview
737701
Homo sapiens
?
-
-
-
-
2.7.7.41
additional information
mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS2, overview
737701
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.7.41
22
-
assay at room temperature
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.7.41
2.9
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
pH 8.0, 22°C; pH 8.0, 22°C, recombinant CDS1
Homo sapiens
2.7.7.41
2.9
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
pH 8.0, 22°C; pH 8.0, 22°C, recombinant CDS2
Homo sapiens
2.7.7.41
3.2
-
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
pH 8.0, 22°C; pH 8.0, 22°C, recombinant CDS1
Homo sapiens
2.7.7.41
8
-
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
pH 8.0, 22°C; pH 8.0, 22°C, recombinant CDS2
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.41
8
-
assay at
Homo sapiens
General Information
EC Number
General Information
Commentary
Organism
2.7.7.41
metabolism
the two isoforms of human CDS, CDS1 and CDS2, show different acyl chain specificities for its lipid substrate. CDS1 and CDS2 can create different CDP-DAG pools that may serve to enrich different phospholipid species with specific acyl chains; the two isoforms of human CDS, CDS1 and CDS2, show different acyl chain specificities for its lipid substrate. CDS1 and CDS2 can create different CDP-DAG pools that may serve to enrich different phospholipid species with specific acyl chains
Homo sapiens
2.7.7.41
physiological function
CDP-diacylglycerol synthases (CDS) are critical enzymes that catalyze the formation of CDP-diacylglycerol (CDP-DAG) from phosphatidic acid; CDP-diacylglycerol synthases (CDS) are critical enzymes that catalyze the formation of CDP-diacylglycerol (CDP-DAG) from phosphatidic acid; isoforms CDS1 and CDS2 could create different CDP-diacylglycerol pools that may serve to enrich different phospholipid species with specific acyl chains. CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested, and shows no acyl chain-dependent inhibition. Both isoforms CDS1 and CDS2 are inhibited by their anionic phospholipid end products; isoforms CDS1 and CDS2 could create different CDP-diacylglycerol pools that may serve to enrich different phospholipid species with specific acyl chains. CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid. Inhibition of CDS2 by phosphatidylinositol is also acyl chain-dependent, with the strongest inhibition seen with the 1-stearoyl-2-arachidonoyl species. Both isoforms CDS1 and CDS2 are inhibited by their anionic phospholipid end products
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.7.41
metabolism
the two isoforms of human CDS, CDS1 and CDS2, show different acyl chain specificities for its lipid substrate. CDS1 and CDS2 can create different CDP-DAG pools that may serve to enrich different phospholipid species with specific acyl chains
Homo sapiens
2.7.7.41
physiological function
CDP-diacylglycerol synthases (CDS) are critical enzymes that catalyze the formation of CDP-diacylglycerol (CDP-DAG) from phosphatidic acid; isoforms CDS1 and CDS2 could create different CDP-diacylglycerol pools that may serve to enrich different phospholipid species with specific acyl chains. CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested, and shows no acyl chain-dependent inhibition. Both isoforms CDS1 and CDS2 are inhibited by their anionic phospholipid end products
Homo sapiens
2.7.7.41
physiological function
CDP-diacylglycerol synthases (CDS) are critical enzymes that catalyze the formation of CDP-diacylglycerol (CDP-DAG) from phosphatidic acid; isoforms CDS1 and CDS2 could create different CDP-diacylglycerol pools that may serve to enrich different phospholipid species with specific acyl chains. CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid. Inhibition of CDS2 by phosphatidylinositol is also acyl chain-dependent, with the strongest inhibition seen with the 1-stearoyl-2-arachidonoyl species. Both isoforms CDS1 and CDS2 are inhibited by their anionic phospholipid end products
Homo sapiens