BRENDA - Enzyme Database

Evidence for a catalytically and kinetically competent enzyme-substrate cross-linked intermediate in catalysis by lipoyl synthase

Lanz, N.D.; Pandelia, M.E.; Kakar, E.S.; Lee, K.H.; Krebs, C.; Booker, S.J.; Biochemistry 53, 4557-4572 (2014) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.8.1.8
protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] cluster
Thermus thermophilus
-
protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] cluster
-
-
?
2.8.1.8
protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] cluster
Escherichia coli
-
protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] cluster
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.8.1.8
Escherichia coli
-
-
-
2.8.1.8
Thermus thermophilus
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.8.1.8
biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-octanoyl)Lys-Ala-Ala-Ser-As + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] ferredoxin
-
737696
Thermus thermophilus
biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-lipoyl)Lys-Ala-Ala-Ser-As + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] ferredoxin
-
-
-
?
2.8.1.8
biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-octanoyl)Lys-Ala-Ala-Ser-As + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] ferredoxin
-
737696
Escherichia coli
biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-lipoyl)Lys-Ala-Ala-Ser-As + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] ferredoxin
-
-
-
?
2.8.1.8
Glu-Ser-Val-(N6-octanoyl)Lys-Ala-Ala-Ser-Asp + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] dithionite
-
737696
Thermus thermophilus
Glu-Ser-Val-(N6-lipoyl)Lys-Ala-Ala-Ser-Asp + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] dithionite
-
-
-
?
2.8.1.8
Glu-Ser-Val-(N6-octanoyl)Lys-Ala-Ala-Ser-Asp + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] dithionite
-
737696
Escherichia coli
Glu-Ser-Val-(N6-lipoyl)Lys-Ala-Ala-Ser-Asp + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] dithionite
-
-
-
?
2.8.1.8
protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] cluster
-
737696
Thermus thermophilus
protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] cluster
-
-
-
?
2.8.1.8
protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] cluster
-
737696
Escherichia coli
protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] cluster
-
-
-
?
2.8.1.8
[8,8,8-2H3]-octanoyl-H protein + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] dithionite
-
737696
Thermus thermophilus
[8,8,8-2H3]-lipoyl-H protein + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] dithionite
-
-
-
?
2.8.1.8
[8,8,8-2H3]-octanoyl-H protein + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] dithionite
-
737696
Escherichia coli
[8,8,8-2H3]-lipoyl-H protein + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] dithionite
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.8.1.8
S-adenosyl-L-methionine
-
Thermus thermophilus
2.8.1.8
S-adenosyl-L-methionine
-
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.8.1.8
S-adenosyl-L-methionine
-
Thermus thermophilus
2.8.1.8
S-adenosyl-L-methionine
-
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.8.1.8
protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] cluster
Thermus thermophilus
-
protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] cluster
-
-
?
2.8.1.8
protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] cluster
Escherichia coli
-
protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] cluster
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.8.1.8
biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-octanoyl)Lys-Ala-Ala-Ser-As + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] ferredoxin
-
737696
Thermus thermophilus
biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-lipoyl)Lys-Ala-Ala-Ser-As + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] ferredoxin
-
-
-
?
2.8.1.8
biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-octanoyl)Lys-Ala-Ala-Ser-As + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] ferredoxin
-
737696
Escherichia coli
biotin-PEG-Glu-Ser-Val-([8,8,8-2H3]-N6-lipoyl)Lys-Ala-Ala-Ser-As + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] ferredoxin
-
-
-
?
2.8.1.8
Glu-Ser-Val-(N6-octanoyl)Lys-Ala-Ala-Ser-Asp + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] dithionite
-
737696
Thermus thermophilus
Glu-Ser-Val-(N6-lipoyl)Lys-Ala-Ala-Ser-Asp + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] dithionite
-
-
-
?
2.8.1.8
Glu-Ser-Val-(N6-octanoyl)Lys-Ala-Ala-Ser-Asp + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] dithionite
-
737696
Escherichia coli
Glu-Ser-Val-(N6-lipoyl)Lys-Ala-Ala-Ser-Asp + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] dithionite
-
-
-
?
2.8.1.8
protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] cluster
-
737696
Thermus thermophilus
protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] cluster
-
-
-
?
2.8.1.8
protein N6-(octanoyl)lysine + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] cluster
-
737696
Escherichia coli
protein N6-(lipoyl)lysine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] cluster
-
-
-
?
2.8.1.8
[8,8,8-2H3]-octanoyl-H protein + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] dithionite
-
737696
Thermus thermophilus
[8,8,8-2H3]-lipoyl-H protein + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] dithionite
-
-
-
?
2.8.1.8
[8,8,8-2H3]-octanoyl-H protein + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced [4Fe-4S] dithionite
-
737696
Escherichia coli
[8,8,8-2H3]-lipoyl-H protein + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + oxidized [4Fe-4S] dithionite
-
-
-
?