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Literature summary extracted from

  • Shulga, Y.V.; Loukov, D.; Ivanova, P.T.; Milne, S.B.; Myers, D.S.; Hatch, G.M.; Umeh, G.; Jalan, D.; Fullerton, M.D.; Steinberg, G.R.; Topham, M.K.; Brown, H.A.; Epand, R.M.
    Diacylglycerol kinase delta promotes lipogenesis (2013), Biochemistry, 52, 7766-7776.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.1.107 medicine the isozyme DGKdelta may be a target for cancer chemotherapy Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.107 gene dgkdelta, both splicing variants delta1 and delta2 are recombinantly expressed as FLAG-tagged proteins in enzyme-deficient 3T3-L1 pre-adipocytes, real-time RT-PCR enzyme expression analysis during adipocyte differentiation shows that 3T3-L1 preadipocytes transfected with DGKdelta have no evidence of differentiation to adipocytes Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.107 additional information generation of enzyme-deficient DGKdelta-KO mouse embryonic fibroblasts Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.107 membrane
-
 Mus musculus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol Mus musculus
-
 ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Mus musculus
-
 gene dgkdelta with two variants delta1 and delta2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.107 recombinant FLAG-tagged splicing variants delta1 and delta2 from 3T3-L1 pre-adipocytes by affinity chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.107 brown adipose tissue DGKdelta is the most abundant isoform in murine brown adipose tissue Mus musculus
-
2.7.1.107 embryo
-
 Mus musculus
-
2.7.1.107 fibroblast embryonic Mus musculus
-
2.7.1.107 heart DGKdelta is the most abundant isoform in murine heart tissue Mus musculus
-
2.7.1.107 liver DGKdelta and DGKtheta are the most abundant isoforms in murine liver Mus musculus
-
2.7.1.107 skeletal muscle DGKdelta and DGKzeta are the most abundant isoforms in murine muscle tissue Mus musculus
-
2.7.1.107 white adipose tissue DGKdelta is the most abundant isoform in murine white adipose tissue Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol
-
 Mus musculus ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.107 DGKdelta
-
 Mus musculus
2.7.1.107 diacylglycerol kinase delta
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.107 ATP
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.7.1.107 malfunction transformed mouse embryo fibroblasts from mice that have a genetic deletion of DGKdelta exhibit decreased levels of many phospholipids and neutral lipids compared with wild-type mouse embryonic fibroblasts. DGKdelta knockout leads to down-regulation of enzymes responsible for fatty acid synthesis and lowers the amount of many lipid species within the cell Mus musculus
2.7.1.107 physiological function DGKdelta promotes de novo lipid synthesis is through signal transduction pathways, overview Mus musculus