Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chanduri, M.; Rai, A.; Malla, A.B.; Wu, M.; Fiedler, D.; Mallik, R.; Bhandari, R.
    Inositol hexakisphosphate kinase 1 (IP6K1) activity is required for cytoplasmic dynein-driven transport (2016), Biochem. J., 473, 3031-3047.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.158 expressed in Escherichia coli BL21(DE3) cells Mus musculus
2.7.4.21 gene IP6K1 Mus musculus
2.7.4.21 gene IP6K1 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.21 additional information generation of gene Ip6k1 knockout mutant cells Mus musculus
2.7.4.21 additional information generation of gene Ip6k1 knockout mutant cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.158 cytoplasm
-
 Mus musculus 5737
-
2.7.4.21 endosome
-
 Dictyostelium discoideum 5768
-
2.7.4.21 endosome
-
 Mus musculus 5768
-
2.7.4.21 endosome
-
 Homo sapiens 5768
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.21 Mg2+ required Dictyostelium discoideum
2.7.4.21 Mg2+ required Mus musculus
2.7.4.21 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.158 ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate Mus musculus
-
 ADP + 1D-myo-inositol hexakisphosphate
-
 ?
2.7.1.158 ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate Mus musculus C57BL/6
-
 ADP + 1D-myo-inositol hexakisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.158 Mus musculus Q6PD10
-
-
2.7.4.21 Dictyostelium discoideum
-
-
-
2.7.4.21 Homo sapiens Q92551
-
-
2.7.4.21 Mus musculus Q6PD10 gene IP6K1
-
2.7.4.21 Mus musculus C57BL/6 Q6PD10 gene IP6K1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.158 glutathione agarose bead chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.158 MEF cell
-
 Mus musculus
-
2.7.4.21 macrophage
-
 Mus musculus
-
2.7.4.21 MEF cell
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.158 ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 Mus musculus ADP + 1D-myo-inositol hexakisphosphate
-
 ?
2.7.1.158 ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 Mus musculus C57BL/6 ADP + 1D-myo-inositol hexakisphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.158 inositol hexakisphosphate kinase 1
-
 Mus musculus
2.7.1.158 IP6K1
-
 Mus musculus
2.7.4.21 inositol hexakisphosphate kinase 1
-
 Dictyostelium discoideum
2.7.4.21 inositol hexakisphosphate kinase 1
-
 Mus musculus
2.7.4.21 inositol hexakisphosphate kinase 1
-
 Homo sapiens
2.7.4.21 IP6K1
-
 Dictyostelium discoideum
2.7.4.21 IP6K1
-
 Mus musculus
2.7.4.21 IP6K1
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.21 ATP
-
 Dictyostelium discoideum
2.7.4.21 ATP
-
 Mus musculus
2.7.4.21 ATP
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.1.158 malfunction cells lacking the enzyme display defects in dynein-dependent trafficking pathways including endosomal sorting, vesicle movement and Golgi maintenance Mus musculus
2.7.1.158 physiological function the enzyme activity is required for cytoplasmic dynein transport Mus musculus
2.7.4.21 evolution in Dictyostelium discoideum, the IP7 target Ser is conserved, but the neighboring Asp and Glu residues are replaced with Thr. These Thr residues may undergo phosphorylation to mimic Asp/Glu and create a consensus site for diphosphorylation Dictyostelium discoideum
2.7.4.21 evolution the absence of diphosphorylation in the IC(1-70)fragment suggests that the Ser-Pro cluster (residues 71-111) is required to facilitate pyrophosphorylation on Ser51. The site of diphosphorylation in mouse IC-2C is well conserved in human and rat, suggesting that the effect of IP7 on dynein is likely to be conserved in these species Mus musculus
2.7.4.21 evolution the absence of diphosphorylation in the IC(1-70)fragment suggests that the Ser-Pro cluster (residues 71-111) is required to facilitate pyrophosphorylation on Ser51. The site of diphosphorylation in mouse IC-2C is well conserved in human and rat, suggesting that the effect of IP7 on dynein is likely to be conserved in these species Homo sapiens
2.7.4.21 malfunction endosomes derived from slime mold lacking inositol diphosphates display reduced dynein-directed microtubule transport. Intermediate chain recruitment to membranes is reduced in cells lacking IP6K1 Dictyostelium discoideum
2.7.4.21 malfunction mammalian cells lacking IP6K1 display defects in dynein-dependent trafficking pathways, including endosomal sorting, vesicle movement, and Golgi maintenance. Expression of catalytically active but not inactive IP6K1 reverses the defects. Intermediate chain recruitment to membranes is reduced in cells lacking IP6K1 Homo sapiens
2.7.4.21 malfunction mammalian cells lacking IP6K1 display defects in dynein-dependent trafficking pathways, including endosomal sorting, vesicle movement, and Golgi maintenance. Expression of catalytically active but not inactive IP6K1 reverses the defects. Intermediate chain recruitment to membranes is reduced in cells lacking IP6K1. Decreased Tfn distribution in the ERC in Ip6k1-/- MEFs might be due to a delay in Tfn trafficking from endosomes. Tfn is held back in early endosomes in cells lacking IP6K1 Mus musculus
2.7.4.21 physiological function generated predominantly by inositol hexakisphosphate kinases (IP6Ks), inositol pyrophosphates can modulate protein function by posttranslational serine diphosphorylation. Ser51 in the dynein intermediate chain is a target for diphosphorylation by IP7, and this modification promotes the interaction of the intermediate chain N-terminus with the p150Glued subunit of dynactin. Involvement of IP6Ks in dynein function, inositol pyrophosphate-mediated diphosphorylation may act as a regulatory signal to enhance dynein-driven transport. Endosomal sorting of Tfn in fibroblasts requires IP6K1 activity, the enzyme activity also is required to maintain Golgi morphology. IP6K1 activity regulates Tfn trafficking, overview Mus musculus
2.7.4.21 physiological function generated predominantly by inositol hexakisphosphate kinases (IP6Ks), inositol pyrophosphates can modulate protein function by posttranslational serine diphosphorylation. Ser51 in the dynein intermediate chain is a target for diphosphorylation by IP7, and this modification promotes the interaction of the intermediate chain N-terminus with the p150Glued subunit of dynactin. Involvement of IP6Ks in dynein function, inositol pyrophosphate-mediated diphosphorylation may act as a regulatory signal to enhance dynein-driven transport. IP6K1 activity regulates Tfn trafficking, overview Homo sapiens
2.7.4.21 physiological function generated predominantly by inositol hexakisphosphate kinases (IP6Ks), inositol pyrophosphates can modulate protein function by posttranslational serine diphosphorylation. Ser51 in the dynein intermediate chain is a target for diphosphorylation by IP7, and this modification promotes the interaction of the intermediate chain N-terminus with the p150Glued subunit of dynactin. Involvement of IP6Ks in dynein function, inositol pyrophosphate-mediated diphosphorylation may act as a regulatory signal to enhance dynein-driven transport. Phagosomal motility requires IP6K1. IP6K1 activity regulates Tfn trafficking, overview Dictyostelium discoideum