Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Clarke, J.H.; Giudici, M.L.; Burke, J.E.; Williams, R.L.; Maloney, D.J.; Marugan, J.; Irvine, R.F.
    The function of phosphatidylinositol 5-phosphate 4-kinase gamma (PI5P4Kgamma) explored using a specific inhibitor that targets the PI5P-binding site (2015), Biochem. J., 466, 359-367.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.149 expressed in Escherichia coli BL21(DE3) cells Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.149 NIH-12848 full designation NCGC00012848-02, specific inhibitor of isoform PI5P4Kgamma, does not inhibit the alpha and beta PI5P4K isoforms at concentrations up to 0.1 mM; inhibitor of isoform PI5P4Kgamma only Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.149 ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate Mus musculus
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.149 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.149 glutathione Sepharose column chromatography Mus musculus
2.7.1.149 glutathione Sepharose column chromatography, and gel filtration Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.149 cortical collecting duct cell
-
 Mus musculus
-
2.7.1.149 kidney cortical collecting duct cell
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.149 ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate
-
 Mus musculus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.149 phosphatidylinositol 5-phosphate 4-kinase
-
 Mus musculus
2.7.1.149 phosphatidylinositol 5-phosphate 4-kinase gamma
-
 Mus musculus
2.7.1.149 PI5P4Kgamma
-
 Mus musculus
2.7.1.149 PI5P4Kgamma isoform Mus musculus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.149 0.001
-
 at pH 7.5 and 30°C Mus musculus NIH-12848
2.7.1.149 0.001
-
 at 22°C, pH not specified in the publication Mus musculus NIH-12848

Expression

EC Number Organism Comment Expression
2.7.1.149 Mus musculus the expression of isoform PI5P4Kgamma increases when the cells grow to confluence and polarize up