Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Liu, Y.; Lai, Y.C.; Hill, E.V.; Tyteca, D.; Carpentier, S.; Ingvaldsen, A.; Vertommen, D.; Lantier, L.; Foretz, M.; Dequiedt, F.; Courtoy, P.J.; Erneux, C.; Viollet, B.; Shepherd, P.R.; Tavare, J.M.; Jensen, J.; Rider, M.H.
    Phosphatidylinositol 3-phosphate 5-kinase (PIKfyve) is an AMPK target participating in contraction-stimulated glucose uptake in skeletal muscle (2013), Biochem. J., 455, 195-206.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.150 YM201636 a selective PIKfyve inhibitor Didelphis sp.
2.7.1.150 YM201636 a selective PIKfyve inhibitor Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.150 membrane
-
 Didelphis sp. 16020
-
2.7.1.150 membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.150 Mg2+ required Didelphis sp.
2.7.1.150 Mg2+ required Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.150 ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate Didelphis sp.
-
 ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
 ?
2.7.1.150 ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate Rattus norvegicus
-
 ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.150 Didelphis sp.
-
 gene PIKfyve
-
2.7.1.150 Rattus norvegicus D3ZYT8 gene PIKfyve
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.150 phosphoprotein AMP-activated protein kinase stimulates the enzyme by phosphorylation at Ser307 both in vitro and in intact cells Didelphis sp.
2.7.1.150 phosphoprotein AMP-activated protein kinase stimulates the enzyme by phosphorylation at Ser307 both in vitro and in intact cells Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.150 C2C12 cell
-
 Rattus norvegicus
-
2.7.1.150 epitrochlearis muscle the enzyme is increased in contracting versus resting muscles Rattus norvegicus
-
2.7.1.150 kidney
-
 Didelphis sp.
-
2.7.1.150 myotube
-
 Rattus norvegicus
-
2.7.1.150 skeletal muscle
-
 Didelphis sp.
-
2.7.1.150 skeletal muscle
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.150 ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 Didelphis sp. ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
 ?
2.7.1.150 ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 Rattus norvegicus ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.150 FYVE domain-containing phosphatidylinositol 3-phosphate 5-kinase
-
 Didelphis sp.
2.7.1.150 FYVE domain-containing phosphatidylinositol 3-phosphate 5-kinase
-
 Rattus norvegicus
2.7.1.150 phosphatidylinositol 3-phosphate 5-kinase
-
 Didelphis sp.
2.7.1.150 phosphatidylinositol 3-phosphate 5-kinase
-
 Rattus norvegicus
2.7.1.150 PIKfyve
-
 Didelphis sp.
2.7.1.150 PIKfyve
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.150 ATP
-
 Didelphis sp.
2.7.1.150 ATP
-
 Rattus norvegicus

General Information

EC Number General Information Comment Organism
2.7.1.150 malfunction enzyme inhibition reduces contraction- and AICAriboside (5-amino-4-imidazolecarboxamide riboside)-stimulated glucose uptake. PIKfyve knockdown in C2C12 myotubes reduced AICAriboside-stimulated glucose transport Rattus norvegicus
2.7.1.150 physiological function the enzyme activity is implicated in be involved in contraction/AMPK (AMP-activated protein kinase)-stimulated glucose uptake in skeletal muscle rather than insulin-stimulated glucose uptake. The enzyme is an AMPK substrate whose phosphorylation at Ser307 promotes PIKfyve translocation to endosomes for PtdIns(3,5)P2 synthesis to facilitate GLUT4 (glucose transporter 4) translocation Rattus norvegicus
2.7.1.150 physiological function the enzyme activity is required for the stimulation of skeletal muscle glucose uptake by contraction/AMPK activation. In opossum kidney cells, wild-type, but not S307A mutant, PIKfyve is recruited to endosomal vesicles in response to AMPK activation Didelphis sp.