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Literature summary extracted from

  • Tuohy, M. G.; Puls, J.; Claeyssens, M.; Vrsanska, M.; Coughlan, M.P.
    The xylan-degrading enzyme system of Talaromyces emersonii: novel enzymes with activity against aryl beta-D-xylosides and unsubstituted xylans (1993), Biochem. J., 290, 515-523.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 0.4
-
 4-nitrophenyl-4-O-beta-D-xylopyranosyl-beta-D-xylopyranose pH 5, 50°C, xylanase II Rasamsonia emersonii
3.2.1.8 1.4
-
 4-nitrophenyl xylopyranoside pH 5, 50°C, xylanase III Rasamsonia emersonii
3.2.1.8 1.8
-
 2-nitrophenyl xylopyranoside pH 5, 50°C, xylanase II Rasamsonia emersonii
3.2.1.8 3.3
-
 2-nitrophenyl xylopyranoside pH 5, 50°C, xylanase III Rasamsonia emersonii
3.2.1.8 32.9
-
 4-nitrophenyl xylopyranoside pH 5, 50°C, xylanase II Rasamsonia emersonii
3.2.1.37 0.1
-
 2-nitrophenyl xylopyranoside pH 5, 50°C, xylanase I Rasamsonia emersonii
3.2.1.37 0.13
-
 4-nitrophenyl xylopyranoside pH 5, 50°C, xylanase I Rasamsonia emersonii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
 Rasamsonia emersonii
-
-
3.2.1.37 extracellular
-
 Rasamsonia emersonii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 49800
-
-
 Rasamsonia emersonii
3.2.1.8 54200
-
 xylanase III, SDS-PAGE Rasamsonia emersonii
3.2.1.8 131000
-
 xylanase II, gel filtration Rasamsonia emersonii
3.2.1.37 97500
-
-
 Rasamsonia emersonii
3.2.1.37 181000
-
 gel filtration Rasamsonia emersonii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Rasamsonia emersonii
-
-
-
3.2.1.37 Rasamsonia emersonii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
 Rasamsonia emersonii
3.2.1.37
-
 Rasamsonia emersonii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 0.2
-
 substrate: 4-nitrophenyl xylopyranoside, pH 5, 50°C, xylanase III Rasamsonia emersonii
3.2.1.8 0.4
-
 substrate: 4-nitrophenyl-4-O-beta-D-xylopyranosyl-beta-D-xylopyranose, pH 5, 50°C, xylanase III Rasamsonia emersonii
3.2.1.8 2.9
-
 substrate: 4-nitrophenyl xylopyranoside, pH 5, 50°C, xylanase II Rasamsonia emersonii
3.2.1.8 92.6
-
 substrate: 2-nitrophenyl xylopyranoside, pH 5, 50°C, xylanase III Rasamsonia emersonii
3.2.1.8 163.1
-
 substrate: 2-nitrophenyl xylopyranoside, pH 5, 50°C, xylanase II Rasamsonia emersonii
3.2.1.8 287.5
-
 substrate: 4-nitrophenyl-4-O-beta-D-xylopyranosyl-beta-D-xylopyranose, pH 5, 50°C, xylanase II Rasamsonia emersonii
3.2.1.37 3
-
 substrate: 4-nitrophenyl xylopyranoside, pH 5, 50°C, xylanase II Rasamsonia emersonii
3.2.1.37 3.6
-
 substrate: 4-nitrophenyl-4-O-beta-D-xylopyranosyl-beta-D-xylopyranose, pH 5, 50°C, xylanase II Rasamsonia emersonii
3.2.1.37 44.8
-
 substrate: 2-nitrophenyl xylopyranoside, pH 5, 50°C, xylanase II Rasamsonia emersonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 2-nitrophenyl xylopyranoside + H2O
-
 Rasamsonia emersonii 2-nitrophenol + xylopyranose
-
 ?
3.2.1.8 4-nitrophenyl xylopyranoside + H2O
-
 Rasamsonia emersonii 4-nitrophenol + xylopyranose
-
 ?
3.2.1.8 4-nitrophenyl-4-O-beta-D-xylopyranosyl-beta-D-xylopyranose + H2O
-
 Rasamsonia emersonii ?
-
 ?
3.2.1.8 xylan + H2O lenzing xylan Rasamsonia emersonii xylobiose + xylotriose + ? and small amounts of xylotetraose and xylopentaose ?
3.2.1.37 2-nitrophenyl xylopyranoside + H2O
-
 Rasamsonia emersonii 2-nitrophenol + xylopyranose
-
 ?
3.2.1.37 4-nitrophenyl xylopyranoside + H2O
-
 Rasamsonia emersonii 4-nitrophenol + xylopyranose
-
 ?
3.2.1.37 xylan + H2O lenzing xylan or wheat straw xylan Rasamsonia emersonii xylose
-
 ?
3.2.1.37 xylobiose + H2O
-
 Rasamsonia emersonii 2 D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 dimer 2 * 49800, xylanase II Rasamsonia emersonii
3.2.1.8 monomer 1 * 54200, xylanase III Rasamsonia emersonii
3.2.1.37 dimer 2 * 97500, SDS-PAGE Rasamsonia emersonii

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase II
-
 Rasamsonia emersonii
3.2.1.8 xylanase III
-
 Rasamsonia emersonii
3.2.1.37 Xyl I
-
 Rasamsonia emersonii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 61
-
 4-nitrophenyl xylopyranoside pH 5, 50°C, xylanase III Rasamsonia emersonii
3.2.1.8 345
-
 2-nitrophenyl xylopyranoside pH 5, 50°C, xylanase III Rasamsonia emersonii
3.2.1.8 523
-
 2-nitrophenyl xylopyranoside pH 5, 50°C, xylanase II Rasamsonia emersonii
3.2.1.8 898
-
 4-nitrophenyl xylopyranoside pH 5, 50°C, xylanase II Rasamsonia emersonii
3.2.1.37 96
-
 2-nitrophenyl xylopyranoside pH 5, 50°C, xylanase I Rasamsonia emersonii
3.2.1.37 426
-
 4-nitrophenyl xylopyranoside pH 5, 50°C, xylanase I Rasamsonia emersonii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 3.5
-
 xylanase III Rasamsonia emersonii
3.2.1.8 4.2
-
 xylanase II Rasamsonia emersonii
3.2.1.37 3.5
-
-
 Rasamsonia emersonii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Rasamsonia emersonii xylanase III, isoelectric focusing
-
 4.2
3.2.1.8 Rasamsonia emersonii xylanase II, isoelectric focusing
-
 5.3
3.2.1.37 Rasamsonia emersonii isoelectric focusing
-
 8.9

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.8 27
-
 4-nitrophenyl xylopyranoside pH 5, 50°C, xylanase II Rasamsonia emersonii
3.2.1.8 43
-
 4-nitrophenyl xylopyranoside pH 5, 50°C, xylanase III Rasamsonia emersonii
3.2.1.8 104
-
 2-nitrophenyl xylopyranoside pH 5, 50°C, xylanase III Rasamsonia emersonii
3.2.1.8 292
-
 2-nitrophenyl xylopyranoside pH 5, 50°C, xylanase II Rasamsonia emersonii
3.2.1.8 7190
-
 4-nitrophenyl-4-O-beta-D-xylopyranosyl-beta-D-xylopyranose pH 5, 50°C, xylanase II Rasamsonia emersonii
3.2.1.37 1066
-
 2-nitrophenyl xylopyranoside pH 5, 50°C, xylanase I Rasamsonia emersonii
3.2.1.37 3275
-
 4-nitrophenyl xylopyranoside pH 5, 50°C, xylanase I Rasamsonia emersonii