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Literature summary extracted from

  • Barth, M.; Oeser, T.; Wei, R.; Then, J.; Schmidt, J.; Zimmermann, W.
    Effect of hydrolysis products on the enzymatic degradation of polyethylene terephthalate nanoparticles by a polyester hydrolase from Thermobifida fusca (2015), Biochem. Eng. J., 93, 222-228.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.101 bis(2-hydroxyethyl) terephthalic acid competitive inhibition of the hydrolytic activity against PET nanoparticles Thermobifida fusca
3.1.1.101 ethylene terephthalate competitive inhibition of the hydrolytic activity against PET nanoparticles Thermobifida fusca
3.1.1.102 bis(2-hydroxyethyl) terephthalic acid competitive inhibition of the hydrolytic activity against PET nanoparticles Thermobifida fusca
3.1.1.102 ethylene terephthalate competitive inhibition of the hydrolytic activity against PET nanoparticles Thermobifida fusca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.102 3.9
-
 bis(2-hydroxyethyl) terephthalic acid pH 8.5, 60°C Thermobifida fusca
3.1.1.102 7.3
-
 ethylene terephthalate pH 8.5, 60°C Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.101 Thermobifida fusca E5BBQ3 cf. EC 3.1.1.74
-
3.1.1.102 Thermobifida fusca E5BBQ3 cf. EC 3.1.1.74
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.101 additional information enzyme additionally catalyzes hydrolysis of ethylene terephthalate and bis(2-hydroxyethyl) terephthalic acid, reaction of EC 3.1.1.102 Thermobifida fusca ?
-
 ?
3.1.1.102 bis(2-hydroxyethyl) terephthalic acid + 2 H2O
-
 Thermobifida fusca terephthalic acid + 2 ethylene glycol
-
 ?
3.1.1.102 ethylene terephthalate + H2O
-
 Thermobifida fusca terephthalate + ethylene glycol
-
 ?
3.1.1.102 additional information enzyme additionally catalyzes hydrolysis of poly(ethylene terephthalate), reaction of EC 3.1.1.101 Thermobifida fusca ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.102 0.31
-
 ethylene terephthalate pH 8.5, 60°C Thermobifida fusca
3.1.1.102 26.8
-
 bis(2-hydroxyethyl) terephthalic acid pH 8.5, 60°C Thermobifida fusca