BRENDA - Enzyme Database

Stoichiometry of ATP hydrolysis and chlorophyllide formation of dark-operative protochlorophyllide oxidoreductase from Rhodobacter capsulatus

Nomata, J.; Terauchi, K.; Fujita, Y.; Biochem. Biophys. Res. Commun. 470, 704-709 (2016)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.1.33
protochlorophyllide + NADPH + H+
Rhodobacter capsulatus
-
chlorophyllide a + NADP+
-
-
?
1.3.7.15
protochlorophyllide + reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + H+
Rhodobacter capsulatus
-
chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.1.33
Rhodobacter capsulatus
-
-
-
1.3.7.15
Rhodobacter capsulatus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.3.1.33
Strep-Tactin column chromatography
Rhodobacter capsulatus
1.3.7.15
Strep-Tactin, column chromatography
Rhodobacter capsulatus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.33
additional information
the enzyme consists of an ATP-dependent reductase component, L-protein (a BchL dimer), and a catalytic component, NB-protein (a BchNeBchB heterotetramer). The L-protein transfers electrons to the NB-protein to reduce Pchlide, which is coupled with ATP hydrolysis
737615
Rhodobacter capsulatus
?
-
-
-
-
1.3.1.33
protochlorophyllide + NADPH + H+
-
737615
Rhodobacter capsulatus
chlorophyllide a + NADP+
-
-
-
?
1.3.7.15
protochlorophyllide + reduced dithionite + ATP + H2O + H+
-
737615
Rhodobacter capsulatus
chlorophyllide a + oxidized dithionite + ADP + phosphate
-
-
-
?
1.3.7.15
protochlorophyllide + reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + H+
-
737615
Rhodobacter capsulatus
chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.33
ATP
-
Rhodobacter capsulatus
1.3.1.33
NADPH
-
Rhodobacter capsulatus
1.3.1.33
[4Fe-4S]-center
-
Rhodobacter capsulatus
1.3.7.15
[4Fe-4S]-center
-
Rhodobacter capsulatus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.33
ATP
-
Rhodobacter capsulatus
1.3.1.33
NADPH
-
Rhodobacter capsulatus
1.3.1.33
[4Fe-4S]-center
-
Rhodobacter capsulatus
1.3.7.15
[4Fe-4S]-center
-
Rhodobacter capsulatus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.1.33
protochlorophyllide + NADPH + H+
Rhodobacter capsulatus
-
chlorophyllide a + NADP+
-
-
?
1.3.7.15
protochlorophyllide + reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + H+
Rhodobacter capsulatus
-
chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.1.33
Strep-Tactin column chromatography
Rhodobacter capsulatus
1.3.7.15
Strep-Tactin, column chromatography
Rhodobacter capsulatus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.33
additional information
the enzyme consists of an ATP-dependent reductase component, L-protein (a BchL dimer), and a catalytic component, NB-protein (a BchNeBchB heterotetramer). The L-protein transfers electrons to the NB-protein to reduce Pchlide, which is coupled with ATP hydrolysis
737615
Rhodobacter capsulatus
?
-
-
-
-
1.3.1.33
protochlorophyllide + NADPH + H+
-
737615
Rhodobacter capsulatus
chlorophyllide a + NADP+
-
-
-
?
1.3.7.15
protochlorophyllide + reduced dithionite + ATP + H2O + H+
-
737615
Rhodobacter capsulatus
chlorophyllide a + oxidized dithionite + ADP + phosphate
-
-
-
?
1.3.7.15
protochlorophyllide + reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + H+
-
737615
Rhodobacter capsulatus
chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
-
-
?