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Literature summary extracted from

  • Tripathi, S.; Agarwal, A.; Ramachandran, R.
    Mutational analysis of Mycobacterium tuberculosis lysine epsilon-aminotransferase and inhibitor co-crystal structures, reveals distinct binding modes (2015), Biochem. Biophys. Res. Commun., 463, 154-160.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.36 strcutures of mutants T330S, E243A, N328A, to 2.7-1.95 A resolution Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.36 E243A mutation in acive-site residue. Mutant retains activity, but binds alpha-ketoglutarate in a different conformation Mycobacterium tuberculosis
2.6.1.36 N328A mutation in acive-site residue. Mutant binds pyridoxal 5'-phospate but is inactive Mycobacterium tuberculosis
2.6.1.36 T330A mutation in acive-site residue, mutant cannot bind pyridoxal 5'-phospate and is inactive Mycobacterium tuberculosis
2.6.1.36 T330S mutation in acive-site residue, mutant cannot bind pyridoxal 5'-phospate and is inactive Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.36 1.2
-
 L-lysine wild-type, pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis
2.6.1.36 1.6
-
 2-oxoglutarate mutant E243A, pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis
2.6.1.36 1.7
-
 L-lysine mutant E243A, pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis
2.6.1.36 2.3
-
 2-oxoglutarate wild-type, pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.36 Mycobacterium tuberculosis P9WQ77
-
-
2.6.1.36 Mycobacterium tuberculosis ATCC 25618 P9WQ77
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.36 L-lysine + 2-oxoglutarate
-
 Mycobacterium tuberculosis (S)-2-amino-6-oxohexanoate + L-glutamate
-
 ?
2.6.1.36 L-lysine + 2-oxoglutarate
-
 Mycobacterium tuberculosis ATCC 25618 (S)-2-amino-6-oxohexanoate + L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.36 LAT
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.36 0.92
-
 L-lysine wild-type, pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis
2.6.1.36 1
-
 2-oxoglutarate mutant E243A, pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis
2.6.1.36 1.17
-
 L-lysine mutant E243A, pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis
2.6.1.36 1.43
-
 2-oxoglutarate wild-type, pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis