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Literature summary extracted from

  • Strazdaite-Zieliene, Z.; Zajanckauskaite, A.; Kaliniene, L.; Meskys, R.; Truncaite, L.
    A mutation in the gene for polynucleotide kinase of bacteriophage T4 K10 affects mRNA processing (2014), Arch. Virol., 159, 327-331.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.78 expressed in Escherichia coli C41(DE3) cells Tequatrovirus T4

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.78 G14D the mutation impairs the 5`-kinase activity of the enzyme in vivo as well as in vitro and leads to diminished processing at secondary sites of several RegB-cleaved transcripts Tequatrovirus T4
2.7.1.78 R229H the mutant shows slightly reduced wild type activity Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA Tequatrovirus T4
-
 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA Tequatrovirus T4 K10
-
 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA Tequatrovirus T4
-
 ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA Tequatrovirus T4 K10
-
 ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 additional information Tequatrovirus T4 the enzyme catalyzes both, the phosphorylation of 5‘-hydroxyl termini and the hydrolysis of 3‘-phosphomonoesters and 2‘,3‘-cyclic phosphodiesters of polynucleotides ?
-
 ?
2.7.1.78 additional information Tequatrovirus T4 K10 the enzyme catalyzes both, the phosphorylation of 5‘-hydroxyl termini and the hydrolysis of 3‘-phosphomonoesters and 2‘,3‘-cyclic phosphodiesters of polynucleotides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Tequatrovirus T4
-
-
-
2.7.1.78 Tequatrovirus T4 K10
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.78 His-Spin Protein Miniprep column chromatography, and gel filtration Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Tequatrovirus T4 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Tequatrovirus T4 K10 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA
-
 Tequatrovirus T4 ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA
-
 Tequatrovirus T4 K10 ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 additional information the enzyme catalyzes both, the phosphorylation of 5‘-hydroxyl termini and the hydrolysis of 3‘-phosphomonoesters and 2‘,3‘-cyclic phosphodiesters of polynucleotides Tequatrovirus T4 ?
-
 ?
2.7.1.78 additional information the enzyme catalyzes both, the phosphorylation of 5‘-hydroxyl termini and the hydrolysis of 3‘-phosphomonoesters and 2‘,3‘-cyclic phosphodiesters of polynucleotides Tequatrovirus T4 K10 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.78 PNK
-
 Tequatrovirus T4