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Literature summary extracted from

  • Xu, X.L.; Chen, S.; Salinas, N.D.; Tolia, N.H.; Grant, G.A.
    Comparison of type 1 D-3-phosphoglycerate dehydrogenases reveals unique regulation in pathogenic Mycobacteria (2015), Arch. Biochem. Biophys., 570, 32-39.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.95 expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis
1.1.1.95 expressed in Escherichia coli BL21(DE3) cells Homo sapiens
1.1.1.95 expressed in Escherichia coli BL21(DE3) cells Mycolicibacterium smegmatis
1.1.1.95 expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum
1.1.1.95 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis
1.1.1.95 expressed in Escherichia coli BL21(DE3) cells Mycobacterium marinum
1.1.1.95 expressed in Escherichia coli BL21(DE3) cells Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.95 L-serine
-
 Bacillus subtilis
1.1.1.95 L-serine
-
 Corynebacterium glutamicum
1.1.1.95 L-serine
-
 Homo sapiens
1.1.1.95 L-serine
-
 Mycobacterium marinum
1.1.1.95 L-serine
-
 Mycobacterium tuberculosis
1.1.1.95 L-serine
-
 Mycolicibacterium smegmatis
1.1.1.95 L-serine
-
 Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.95 0.003
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Homo sapiens
1.1.1.95 0.004
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Corynebacterium glutamicum
1.1.1.95 0.008
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycobacterium marinum
1.1.1.95 0.014
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Streptomyces coelicolor
1.1.1.95 0.015
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Bacillus subtilis
1.1.1.95 0.025
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
1.1.1.95 0.031
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Bacillus subtilis
1.1.1.95 0.041
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycobacterium marinum
1.1.1.95 0.049
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycolicibacterium smegmatis
1.1.1.95 0.065
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Corynebacterium glutamicum
1.1.1.95 0.127
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Homo sapiens
1.1.1.95 0.153
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
1.1.1.95 0.162
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Streptomyces coelicolor
1.1.1.95 0.292
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.95 55000
-
-
 Streptomyces coelicolor
1.1.1.95 57200
-
-
 Bacillus subtilis
1.1.1.95 57200
-
-
 Homo sapiens
1.1.1.95 207300
-
 analytical ultracentrifugation Bacillus subtilis
1.1.1.95 207300
-
 analytical ultracentrifugation Homo sapiens
1.1.1.95 207300
-
 analytical ultracentrifugation Mycolicibacterium smegmatis
1.1.1.95 207300
-
 analytical ultracentrifugation Corynebacterium glutamicum
1.1.1.95 207300
-
 analytical ultracentrifugation Mycobacterium tuberculosis
1.1.1.95 207300
-
 analytical ultracentrifugation Mycobacterium marinum
1.1.1.95 207300
-
 analytical ultracentrifugation Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.95 3-phospho-D-glycerate + NAD+ Bacillus subtilis
-
 3-phosphonooxypyruvate + NADH + H+
-
 ?
1.1.1.95 3-phospho-D-glycerate + NAD+ Homo sapiens
-
 3-phosphonooxypyruvate + NADH + H+
-
 ?
1.1.1.95 3-phospho-D-glycerate + NAD+ Mycolicibacterium smegmatis
-
 3-phosphonooxypyruvate + NADH + H+
-
 ?
1.1.1.95 3-phospho-D-glycerate + NAD+ Corynebacterium glutamicum
-
 3-phosphonooxypyruvate + NADH + H+
-
 ?
1.1.1.95 3-phospho-D-glycerate + NAD+ Mycobacterium tuberculosis
-
 3-phosphonooxypyruvate + NADH + H+
-
 ?
1.1.1.95 3-phospho-D-glycerate + NAD+ Mycobacterium marinum
-
 3-phosphonooxypyruvate + NADH + H+
-
 ?
1.1.1.95 3-phospho-D-glycerate + NAD+ Streptomyces coelicolor
-
 3-phosphonooxypyruvate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.95 Bacillus subtilis
-
-
-
1.1.1.95 Corynebacterium glutamicum
-
-
-
1.1.1.95 Homo sapiens
-
-
-
1.1.1.95 Mycobacterium marinum
-
-
-
1.1.1.95 Mycobacterium tuberculosis
-
-
-
1.1.1.95 Mycolicibacterium smegmatis
-
-
-
1.1.1.95 Streptomyces coelicolor Q9Z564
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.95 Talon cobalt based immobilized metal affinity column chromatography Bacillus subtilis
1.1.1.95 Talon cobalt based immobilized metal affinity column chromatography Homo sapiens
1.1.1.95 Talon cobalt based immobilized metal affinity column chromatography Mycolicibacterium smegmatis
1.1.1.95 Talon cobalt based immobilized metal affinity column chromatography Corynebacterium glutamicum
1.1.1.95 Talon cobalt based immobilized metal affinity column chromatography Mycobacterium tuberculosis
1.1.1.95 Talon cobalt based immobilized metal affinity column chromatography Mycobacterium marinum
1.1.1.95 Talon cobalt based immobilized metal affinity column chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.95 3-phospho-D-glycerate + NAD+
-
 Bacillus subtilis 3-phosphonooxypyruvate + NADH + H+
-
 ?
1.1.1.95 3-phospho-D-glycerate + NAD+
-
 Homo sapiens 3-phosphonooxypyruvate + NADH + H+
-
 ?
1.1.1.95 3-phospho-D-glycerate + NAD+
-
 Mycolicibacterium smegmatis 3-phosphonooxypyruvate + NADH + H+
-
 ?
1.1.1.95 3-phospho-D-glycerate + NAD+
-
 Corynebacterium glutamicum 3-phosphonooxypyruvate + NADH + H+
-
 ?
1.1.1.95 3-phospho-D-glycerate + NAD+
-
 Mycobacterium tuberculosis 3-phosphonooxypyruvate + NADH + H+
-
 ?
1.1.1.95 3-phospho-D-glycerate + NAD+
-
 Mycobacterium marinum 3-phosphonooxypyruvate + NADH + H+
-
 ?
1.1.1.95 3-phospho-D-glycerate + NAD+
-
 Streptomyces coelicolor 3-phosphonooxypyruvate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.95 homotetramer 4 * 55000, calculated from amino acid sequence Streptomyces coelicolor
1.1.1.95 homotetramer 4 * 57200, calculated from amino acid sequence Bacillus subtilis
1.1.1.95 homotetramer 4 * 57200, calculated from amino acid sequence Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.95 D-3-phosphoglycerate dehydrogenase
-
 Bacillus subtilis
1.1.1.95 D-3-phosphoglycerate dehydrogenase
-
 Homo sapiens
1.1.1.95 D-3-phosphoglycerate dehydrogenase
-
 Mycolicibacterium smegmatis
1.1.1.95 D-3-phosphoglycerate dehydrogenase
-
 Corynebacterium glutamicum
1.1.1.95 D-3-phosphoglycerate dehydrogenase
-
 Mycobacterium tuberculosis
1.1.1.95 D-3-phosphoglycerate dehydrogenase
-
 Mycobacterium marinum
1.1.1.95 D-3-phosphoglycerate dehydrogenase
-
 Streptomyces coelicolor
1.1.1.95 PGDH
-
 Bacillus subtilis
1.1.1.95 PGDH
-
 Homo sapiens
1.1.1.95 PGDH
-
 Mycolicibacterium smegmatis
1.1.1.95 PGDH
-
 Corynebacterium glutamicum
1.1.1.95 PGDH
-
 Mycobacterium tuberculosis
1.1.1.95 PGDH
-
 Mycobacterium marinum
1.1.1.95 PGDH
-
 Streptomyces coelicolor
1.1.1.95 type 1 D-3-phosphoglycerate dehydrogenase
-
 Bacillus subtilis
1.1.1.95 type 1 D-3-phosphoglycerate dehydrogenase
-
 Homo sapiens
1.1.1.95 type 1 D-3-phosphoglycerate dehydrogenase
-
 Mycolicibacterium smegmatis
1.1.1.95 type 1 D-3-phosphoglycerate dehydrogenase
-
 Corynebacterium glutamicum
1.1.1.95 type 1 D-3-phosphoglycerate dehydrogenase
-
 Mycobacterium tuberculosis
1.1.1.95 type 1 D-3-phosphoglycerate dehydrogenase
-
 Mycobacterium marinum
1.1.1.95 type 1 D-3-phosphoglycerate dehydrogenase
-
 Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.95 41
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Homo sapiens
1.1.1.95 194
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Streptomyces coelicolor
1.1.1.95 198
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Corynebacterium glutamicum
1.1.1.95 311
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Homo sapiens
1.1.1.95 341
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycobacterium marinum
1.1.1.95 350
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Corynebacterium glutamicum
1.1.1.95 464
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Bacillus subtilis
1.1.1.95 567
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Bacillus subtilis
1.1.1.95 576
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Streptomyces coelicolor
1.1.1.95 608
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycobacterium marinum
1.1.1.95 1515
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
1.1.1.95 1575
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycolicibacterium smegmatis
1.1.1.95 2425
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
1.1.1.95 3850
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.95 NAD+
-
 Bacillus subtilis
1.1.1.95 NAD+
-
 Homo sapiens
1.1.1.95 NAD+
-
 Mycolicibacterium smegmatis
1.1.1.95 NAD+
-
 Corynebacterium glutamicum
1.1.1.95 NAD+
-
 Mycobacterium tuberculosis
1.1.1.95 NAD+
-
 Mycobacterium marinum
1.1.1.95 NAD+
-
 Streptomyces coelicolor

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.95 0.016
-
 L-serine in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycolicibacterium smegmatis
1.1.1.95 0.038
-
 L-serine in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Corynebacterium glutamicum
1.1.1.95 0.057
-
 L-serine in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Homo sapiens
1.1.1.95 0.063
-
 L-serine in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Homo sapiens
1.1.1.95 0.066
-
 L-serine in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycobacterium marinum
1.1.1.95 0.082
-
 L-serine in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
1.1.1.95 0.115
-
 L-serine in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Streptomyces coelicolor
1.1.1.95 0.138
-
 L-serine in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Bacillus subtilis
1.1.1.95 0.255
-
 L-serine in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycolicibacterium smegmatis
1.1.1.95 0.581
-
 L-serine in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Streptomyces coelicolor
1.1.1.95 0.628
-
 L-serine in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
1.1.1.95 1.065
-
 L-serine in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycobacterium marinum
1.1.1.95 4.539
-
 L-serine in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Bacillus subtilis
1.1.1.95 5.898
-
 L-serine in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Corynebacterium glutamicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.95 1400
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Streptomyces coelicolor
1.1.1.95 2400
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Homo sapiens
1.1.1.95 3200
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycolicibacterium smegmatis
1.1.1.95 3600
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Streptomyces coelicolor
1.1.1.95 5000
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Corynebacterium glutamicum
1.1.1.95 5300
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Corynebacterium glutamicum
1.1.1.95 6100
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
1.1.1.95 7600
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycobacterium marinum
1.1.1.95 8300
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycobacterium marinum
1.1.1.95 13200
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycolicibacterium smegmatis
1.1.1.95 14000
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Homo sapiens
1.1.1.95 15800
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
1.1.1.95 18000
-
 3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Bacillus subtilis
1.1.1.95 31000
-
 3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Bacillus subtilis