BRENDA - Enzyme Database

Phycobilin biosynthesis: Reductant requirements and product identification for heme oxygenase from Cyanidium caldarium

Rhie, G.; Beale, S.; Arch. Biochem. Biophys. 320, 182-194 (1995)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.15.20
D-ascorbate
99% activity with D-ascorbate as compared to L-ascorbate
Cyanidium caldarium
1.14.15.20
desferrioxamine
6fold increase of activity in the presence of 2.5 mM desferrioxamine
Cyanidium caldarium
1.14.15.20
HClO4
134% activity at 5% (v/v) HClO4
Cyanidium caldarium
1.14.15.20
L-ascorbate
required for activity
Cyanidium caldarium
1.14.15.20
additional information
not activated by EDTA, dehydroascorbate, and phenyledediamine
Cyanidium caldarium
1.14.15.20
Tiron
almost as effective as desferrioxamine
Cyanidium caldarium
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.15.20
soluble
-
Cyanidium caldarium
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Cyanidium caldarium
-
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.20
Cyanidium caldarium
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.15.20
ammonium sulfate precipitation, Blue 2-Sepharose column chromatography, DEAE column chromatography, ferredoxin-Sepharose column chromatography, and Sephadex G-75 gel filtration
Cyanidium caldarium
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.15.20
0.00069
-
unpurified enzyme, at pH 7.6 and 42°C
Cyanidium caldarium
1.14.15.20
0.1403
-
after 203fold purification, at pH 7.6 and 42°C
Cyanidium caldarium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
737548
Cyanidium caldarium
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.15.20
D-ascorbate
99% activity with D-ascorbate as compared to L-ascorbate
Cyanidium caldarium
1.14.15.20
desferrioxamine
6fold increase of activity in the presence of 2.5 mM desferrioxamine
Cyanidium caldarium
1.14.15.20
HClO4
134% activity at 5% (v/v) HClO4
Cyanidium caldarium
1.14.15.20
L-ascorbate
required for activity
Cyanidium caldarium
1.14.15.20
additional information
not activated by EDTA, dehydroascorbate, and phenyledediamine
Cyanidium caldarium
1.14.15.20
Tiron
almost as effective as desferrioxamine
Cyanidium caldarium
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.15.20
soluble
-
Cyanidium caldarium
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Cyanidium caldarium
-
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.20
ammonium sulfate precipitation, Blue 2-Sepharose column chromatography, DEAE column chromatography, ferredoxin-Sepharose column chromatography, and Sephadex G-75 gel filtration
Cyanidium caldarium
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.15.20
0.00069
-
unpurified enzyme, at pH 7.6 and 42°C
Cyanidium caldarium
1.14.15.20
0.1403
-
after 203fold purification, at pH 7.6 and 42°C
Cyanidium caldarium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.20
protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
737548
Cyanidium caldarium
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?