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Literature summary extracted from

  • Matsui, T.; Yoshida, T.; Yoshimura, T.; Nagasawa, T.
    Regioselective carboxylation of 1,3-dihydroxybenzene by 2,6-dihydroxybenzoate decarboxylase of Pandoraea sp. 12B-2 (2006), Appl. Microbiol. Biotechnol., 73, 95-102.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.1.103 synthesis enzyme catalyzes the regioselective carboxylation of phenol, 1,2-dihydroxybenzene and 1,3-dihydroxybenzen to 4-hydroxybenzoate, 2,3-dihydroxybenzoate, and 2,6-dihydroxybenzoate, respectively. When the efficient production of 2,6-dihydroxybenzoate is optimized using whole cells of Pandoraea sp. 12B-2, the productivity of 2,6-dihydroxybenzoate tops out at 1.43 M. No formation of any other products is observed after the carboxylation reaction Pandoraea sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.103 Pandoraea sp.
-
-
-
4.1.1.103 Pandoraea sp. 12B-2
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.103 1,2-dihydroxybenzene + CO2
-
Pandoraea sp. 2,3-dihydroxybenzoate enzyme catalyzes the regioselective carboxylation r
4.1.1.103 1,2-dihydroxybenzene + CO2
-
Pandoraea sp. 12B-2 2,3-dihydroxybenzoate enzyme catalyzes the regioselective carboxylation r
4.1.1.103 1,3-dihydroxybenzene + CO2
-
Pandoraea sp. 2,6-dihydroxybenzoate reaction is reversible. In the presence of 0.1 M KHCO3, 0.5 mM 2,6-dihydroxybenzoate is formed from 12.5 mM 1,3-dihydroxybenzene. With 3 M KHCO3, the molar conversion ratio of 1,3-dihydroxybenzene reaches its highest value (48%) r
4.1.1.103 1,3-dihydroxybenzene + CO2
-
Pandoraea sp. 12B-2 2,6-dihydroxybenzoate reaction is reversible. In the presence of 0.1 M KHCO3, 0.5 mM 2,6-dihydroxybenzoate is formed from 12.5 mM 1,3-dihydroxybenzene. With 3 M KHCO3, the molar conversion ratio of 1,3-dihydroxybenzene reaches its highest value (48%) r
4.1.1.103 2,6-dihydroxybenzoate
-
Pandoraea sp. 1,3-dihydroxybenzene + CO2
-
r
4.1.1.103 2,6-dihydroxybenzoate
-
Pandoraea sp. 12B-2 1,3-dihydroxybenzene + CO2
-
r
4.1.1.103 Phenol + CO2
-
Pandoraea sp. 4-Hydroxybenzoate enzyme catalyzes the regioselective carboxylation r
4.1.1.103 Phenol + CO2
-
Pandoraea sp. 12B-2 4-Hydroxybenzoate enzyme catalyzes the regioselective carboxylation r