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Literature summary extracted from

  • Kahar, U.M.; Ng, C.L.; Chan, K.G.; Goh, K.M.
    Characterization of a type I pullulanase from Anoxybacillus sp. SK3-4 reveals an unusual substrate hydrolysis (2016), Appl. Microbiol. Biotechnol., 100, 6291-6307.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.41 2-mercaptoethanol 2 mM, 143% of initial activtiy Anoxybacillus sp.
3.2.1.41 Tween-20 1% v/v, 143% of initial activtiy Anoxybacillus sp.
3.2.1.142 2-mercaptoethanol 2 mM, 143% of initial activtiy Anoxybacillus sp.
3.2.1.142 Tween-20 1% v/v, 143% of initial activtiy Anoxybacillus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.41 expression in Escherichia coli Anoxybacillus sp.
3.2.1.142 expression in Escherichia coli Anoxybacillus sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.41 additional information amino acids 362-370 of Ask are replaced with the corresponding sequence of type I pullulanase Pul-LM14-2 from Anoxybacillus sp. LM14-2. Unlike wild-type, the mutant enzyme forms reducing sugars on digesting starch Anoxybacillus sp.
3.2.1.142 additional information amino acids 362-370 of Ask are replaced with the corresponding sequence of type I pullulanase Pul-LM14-2 from Anoxybacillus sp. LM14-2. Unlike wild-type, the mutant enzyme forms reducing sugars on digesting starch Anoxybacillus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.41 Ba2+ 2 mM, 54% of initial activtiy Anoxybacillus sp.
3.2.1.41 Co2+ 2 mM, 15% of initial activtiy Anoxybacillus sp.
3.2.1.41 Cu2+ 2 mM, 8% of initial activtiy Anoxybacillus sp.
3.2.1.41 EDTA 2 mM, 68% of initial activtiy Anoxybacillus sp.
3.2.1.41 Fe2+ 2 mM, 6% of initial activtiy Anoxybacillus sp.
3.2.1.41 Mn2+ 2 mM, 40% of initial activtiy Anoxybacillus sp.
3.2.1.41 NH4+ 2 mM, 49% of initial activtiy Anoxybacillus sp.
3.2.1.41 Ni2+ 2 mM, 47% of initial activtiy Anoxybacillus sp.
3.2.1.41 Sr2+ 2 mM, 73% of initial activtiy Anoxybacillus sp.
3.2.1.41 Triton X-100 1% v/v, 72% of initial activtiy Anoxybacillus sp.
3.2.1.41 Urea 2 mM, 57% of initial activtiy Anoxybacillus sp.
3.2.1.41 Zn2+ 2 mM, 22% of initial activtiy Anoxybacillus sp.
3.2.1.142 Ba2+ 2 mM, 54% of initial activtiy Anoxybacillus sp.
3.2.1.142 Co2+ 2 mM, 15% of initial activtiy Anoxybacillus sp.
3.2.1.142 Cu2+ 2 mM, 8% of initial activtiy Anoxybacillus sp.
3.2.1.142 EDTA 2 mM, 68% of initial activtiy Anoxybacillus sp.
3.2.1.142 Fe2+ 2 mM, 6% of initial activtiy Anoxybacillus sp.
3.2.1.142 Mn2+ 2 mM, 40% of initial activtiy Anoxybacillus sp.
3.2.1.142 NH4+ 2 mM, 49% of initial activtiy Anoxybacillus sp.
3.2.1.142 Ni2+ 2 mM, 47% of initial activtiy Anoxybacillus sp.
3.2.1.142 Sr2+ 2 mM, 73% of initial activtiy Anoxybacillus sp.
3.2.1.142 Triton X-100 1% v/v, 72% of initial activtiy Anoxybacillus sp.
3.2.1.142 Urea 2 mM, 57% of initial activtiy Anoxybacillus sp.
3.2.1.142 Zn2+ 2 mM, 22% of initial activtiy Anoxybacillus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.41 K+ 2 mM, 132% of initial activtiy Anoxybacillus sp.
3.2.1.41 Mg2+ 2 mM, 122% of initial activtiy Anoxybacillus sp.
3.2.1.41 Na+ 2 mM, 128% of initial activtiy Anoxybacillus sp.
3.2.1.142 K+ 2 mM, 132% of initial activtiy Anoxybacillus sp.
3.2.1.142 Mg2+ 2 mM, 122% of initial activtiy Anoxybacillus sp.
3.2.1.142 Na+ 2 mM, 128% of initial activtiy Anoxybacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 80000
-
 x * 80000, SDS-PAGE Anoxybacillus sp.
3.2.1.142 80000
-
 x * 80000, SDS-PAGE Anoxybacillus sp.

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.41 DMSO 2 mM, 59% of initial activtiy Anoxybacillus sp.
3.2.1.142 DMSO 2 mM, 59% of initial activtiy Anoxybacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Anoxybacillus sp. EPZ37738
-
-
3.2.1.41 Anoxybacillus sp. SK3-4 EPZ37738
-
-
3.2.1.142 Anoxybacillus sp. EPZ37738
-
-
3.2.1.142 Anoxybacillus sp. SK3-4 EPZ37738
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 additional information the enzyme preferably debranches long branches at alpha-1,6 glycosidic bonds of starch, producing amylose, linear or branched oligosaccharides, but is nonreactive against short branches. In addition, the enzyme acts as limit dextrinase, reaction of EC 3.2.1.142, releasing maltotriose, maltotetraose and maltopentaose from limit dextrin Anoxybacillus sp. ?
-
 ?
3.2.1.41 additional information the enzyme preferably debranches long branches at alpha-1,6 glycosidic bonds of starch, producing amylose, linear or branched oligosaccharides, but is nonreactive against short branches. In addition, the enzyme acts as limit dextrinase, reaction of EC 3.2.1.142, releasing maltotriose, maltotetraose and maltopentaose from limit dextrin Anoxybacillus sp. SK3-4 ?
-
 ?
3.2.1.41 pullulan + H2O
-
 Anoxybacillus sp. maltotriose + ?
-
 ?
3.2.1.41 pullulan + H2O
-
 Anoxybacillus sp. SK3-4 maltotriose + ?
-
 ?
3.2.1.142 limit dextrin + H2O
-
 Anoxybacillus sp. maltotriose + maltotetraose + maltopentaose
-
 ?
3.2.1.142 limit dextrin + H2O
-
 Anoxybacillus sp. SK3-4 maltotriose + maltotetraose + maltopentaose
-
 ?
3.2.1.142 additional information the enzyme preferably debranches long branches at alpha-1,6 glycosidic bonds of starch, producing amylose, linear or branched oligosaccharides, but is nonreactive against short branches. Main reaction is as a type I pullulanase, EC 3.2.1.41 Anoxybacillus sp. ?
-
 ?
3.2.1.142 additional information the enzyme preferably debranches long branches at alpha-1,6 glycosidic bonds of starch, producing amylose, linear or branched oligosaccharides, but is nonreactive against short branches. Main reaction is as a type I pullulanase, EC 3.2.1.41 Anoxybacillus sp. SK3-4 ?
-
 ?
3.2.1.142 pullulan + H2O
-
 Anoxybacillus sp. maltotriose + ?
-
 ?
3.2.1.142 pullulan + H2O
-
 Anoxybacillus sp. SK3-4 maltotriose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.41 ? x * 80000, SDS-PAGE Anoxybacillus sp.
3.2.1.142 ? x * 80000, SDS-PAGE Anoxybacillus sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 Ask
-
 Anoxybacillus sp.
3.2.1.142 Ask
-
 Anoxybacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 60
-
-
 Anoxybacillus sp.
3.2.1.142 60
-
-
 Anoxybacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 30 60 20 min, stable Anoxybacillus sp.
3.2.1.41 60
-
 9 h, 50% residual activity Anoxybacillus sp.
3.2.1.142 30 60 20 min, stable Anoxybacillus sp.
3.2.1.142 60
-
 9 h, 50% residual activity Anoxybacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 6
-
-
 Anoxybacillus sp.
3.2.1.142 6
-
-
 Anoxybacillus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.41 5 6
-
 Anoxybacillus sp.
3.2.1.142 5 6
-
 Anoxybacillus sp.