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Literature summary extracted from

  • Chen, Y.; Yi, D.; Jiang, S.; Wei, D.
    Identification of novel thermostable taurine-pyruvate transaminase from Geobacillus thermodenitrificans for chiral amine synthesis (2016), Appl. Microbiol. Biotechnol., 100, 3101-3111.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.77 additional information incubation th at 50°C for 8 h or at 60°C for 30 min increases activity by up to 2.5fold Geobacillus thermodenitrificans subsp. thermodenitrificans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.77 expression in Escherichia coli Geobacillus thermodenitrificans subsp. thermodenitrificans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.77 molecular modeling of activte site. The active site shows a special large pocket and substrate tunnel Geobacillus thermodenitrificans subsp. thermodenitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.77 5.3
-
 taurine pH 9.0, 37°C Geobacillus thermodenitrificans subsp. thermodenitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.77 45000
-
 x * 45000, SDS-PAGE Geobacillus thermodenitrificans subsp. thermodenitrificans

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.6.1.77 DMSO increase in DMSO concentration up to 10% enhances activity by up to 135% Geobacillus thermodenitrificans subsp. thermodenitrificans
2.6.1.77 Methanol 5%, fast loss of activity Geobacillus thermodenitrificans subsp. thermodenitrificans

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.77 Geobacillus thermodenitrificans subsp. thermodenitrificans A0A0S2VC96
-
-
2.6.1.77 Geobacillus thermodenitrificans subsp. thermodenitrificans DSM 465 A0A0S2VC96
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.77 0.26
-
 substrate (S)-1-methylbenzylamine, pH 9.0, 37°C Geobacillus thermodenitrificans subsp. thermodenitrificans
2.6.1.77 0.52
-
 substrate methanal, pH 9.0, 37°C Geobacillus thermodenitrificans subsp. thermodenitrificans
2.6.1.77 0.67
-
 substrate propanal, pH 9.0, 37°C Geobacillus thermodenitrificans subsp. thermodenitrificans
2.6.1.77 0.68
-
 substrate butanal, pH 9.0, 37°C Geobacillus thermodenitrificans subsp. thermodenitrificans
2.6.1.77 0.68
-
 substrate ethanal, pH 9.0, 37°C Geobacillus thermodenitrificans subsp. thermodenitrificans
2.6.1.77 0.71
-
 substrate glyoxylate, pH 9.0, 37°C Geobacillus thermodenitrificans subsp. thermodenitrificans
2.6.1.77 0.75
-
 substrate glycolaldehyde, pH 9.0, 37°C Geobacillus thermodenitrificans subsp. thermodenitrificans
2.6.1.77 0.78
-
 substrate hexanal, pH 9.0, 37°C Geobacillus thermodenitrificans subsp. thermodenitrificans
2.6.1.77 0.8
-
 substrate pentanal, pH 9.0, 37°C Geobacillus thermodenitrificans subsp. thermodenitrificans
2.6.1.77 10.2
-
 substrate taurine, pH 9.0, 37°C Geobacillus thermodenitrificans subsp. thermodenitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.77 (S)-1-methylbenzylamine + pyruvate no substrate: (R)-enantiomer Geobacillus thermodenitrificans subsp. thermodenitrificans acetophenone + L-alanine
-
 ?
2.6.1.77 (S)-1-methylbenzylamine + pyruvate no substrate: (R)-enantiomer Geobacillus thermodenitrificans subsp. thermodenitrificans DSM 465 acetophenone + L-alanine
-
 ?
2.6.1.77 additional information the enzyme displays broad donor and acceptor ranges for unnatural substrates, with relatively good activity toward ketoses. Enzyme shows a unique mechanism of sulfonate/alpha-carboxylate recognition contributed by Arg163 and Gln160 Geobacillus thermodenitrificans subsp. thermodenitrificans ?
-
 ?
2.6.1.77 additional information the enzyme displays broad donor and acceptor ranges for unnatural substrates, with relatively good activity toward ketoses. Enzyme shows a unique mechanism of sulfonate/alpha-carboxylate recognition contributed by Arg163 and Gln160 Geobacillus thermodenitrificans subsp. thermodenitrificans DSM 465 ?
-
 ?
2.6.1.77 taurine + butanal
-
 Geobacillus thermodenitrificans subsp. thermodenitrificans 2-sulfoacetaldehyde + butyl-1-amine
-
 ?
2.6.1.77 taurine + butanal
-
 Geobacillus thermodenitrificans subsp. thermodenitrificans DSM 465 2-sulfoacetaldehyde + butyl-1-amine
-
 ?
2.6.1.77 taurine + ethanal
-
 Geobacillus thermodenitrificans subsp. thermodenitrificans 2-sulfoacetaldehyde + ethylamine
-
 ?
2.6.1.77 taurine + ethanal
-
 Geobacillus thermodenitrificans subsp. thermodenitrificans DSM 465 2-sulfoacetaldehyde + ethylamine
-
 ?
2.6.1.77 taurine + glycolaldehyde
-
 Geobacillus thermodenitrificans subsp. thermodenitrificans 2-sulfoacetaldehyde + ?
-
 ?
2.6.1.77 taurine + glyoxylate
-
 Geobacillus thermodenitrificans subsp. thermodenitrificans 2-sulfoacetaldehyde + glycine
-
 ?
2.6.1.77 taurine + hexanal
-
 Geobacillus thermodenitrificans subsp. thermodenitrificans 2-sulfoacetaldehyde + hexyl-1-amine
-
 ?
2.6.1.77 taurine + methanal
-
 Geobacillus thermodenitrificans subsp. thermodenitrificans 2-sulfoacetaldehyde + methylamine
-
 ?
2.6.1.77 taurine + pentanal
-
 Geobacillus thermodenitrificans subsp. thermodenitrificans 2-sulfoacetaldehyde + pentylamine
-
 ?
2.6.1.77 taurine + propanal
-
 Geobacillus thermodenitrificans subsp. thermodenitrificans 2-sulfoacetaldehyde + propyl-1-amine
-
 ?
2.6.1.77 taurine + pyruvate
-
 Geobacillus thermodenitrificans subsp. thermodenitrificans 2-sulfoacetaldehyde + L-alanine
-
 ?
2.6.1.77 taurine + pyruvate
-
 Geobacillus thermodenitrificans subsp. thermodenitrificans DSM 465 2-sulfoacetaldehyde + L-alanine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.77 ? x * 45000, SDS-PAGE Geobacillus thermodenitrificans subsp. thermodenitrificans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.77 65
-
-
 Geobacillus thermodenitrificans subsp. thermodenitrificans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.77 37
-
 17% of maximum activity Geobacillus thermodenitrificans subsp. thermodenitrificans
2.6.1.77 70
-
 less than 30% of maximum activity Geobacillus thermodenitrificans subsp. thermodenitrificans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.77 50
-
 8 h, 2.5fold increase in activity Geobacillus thermodenitrificans subsp. thermodenitrificans
2.6.1.77 60
-
 30 min, 2.5fold increase in activity Geobacillus thermodenitrificans subsp. thermodenitrificans
2.6.1.77 70
-
 15 min, almost complete loss of activity Geobacillus thermodenitrificans subsp. thermodenitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.77 9
-
-
 Geobacillus thermodenitrificans subsp. thermodenitrificans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.77 8 10 more than 20% of maximum activity Geobacillus thermodenitrificans subsp. thermodenitrificans