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Literature summary extracted from

  • Tumlirsch, T.; Sznajder, A.; Jendrossek, D.
    Formation of polyphosphate by polyphosphate kinases and its relationship to poly(3-hydroxybutyrate) accumulation in Ralstonia eutropha strain H16 (2015), Appl. Environ. Microbiol., 81, 8277-8293.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.1 ppk1, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain Cupriavidus necator
2.7.4.1 ppk1b, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain Cupriavidus necator
2.7.4.1 ppk2a, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain Cupriavidus necator
2.7.4.1 ppk2b, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain Cupriavidus necator
2.7.4.1 ppk2c, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain Cupriavidus necator
2.7.4.1 ppk2d, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain Cupriavidus necator
2.7.4.1 ppk2e, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain Cupriavidus necator

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.1 additional information construction of a chromosomal deletion strain of the enzyme, formation of polyphosphate and poly(3-hydroxybutyrate) granules in Ralstonia eutropha mutants during growth on NB-gluconate medium, overview Cupriavidus necator

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.1 cytoplasm
-
 Cupriavidus necator 5737
-
2.7.4.1 additional information the enzyme is located near one cell pole apart from polyphosphate granules Cupriavidus necator
-
-
2.7.4.1 additional information the isozyme is located in polyphosphate granules Cupriavidus necator
-
-
2.7.4.1 soluble
-
 Cupriavidus necator
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.1 30700
-
-
 Cupriavidus necator
2.7.4.1 31100
-
-
 Cupriavidus necator
2.7.4.1 33900
-
-
 Cupriavidus necator
2.7.4.1 36200
-
 x * 36200, about, sequence calculation Cupriavidus necator
2.7.4.1 42100
-
-
 Cupriavidus necator
2.7.4.1 78200
-
-
 Cupriavidus necator
2.7.4.1 79600
-
-
 Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.1 ATP + (phosphate)n Cupriavidus necator
-
 ADP + (phosphate)n+1
-
 ?
2.7.4.1 ATP + (phosphate)n Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
 ADP + (phosphate)n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Cupriavidus necator Q0K2G4 gene ppk2 or ppk1b or H16_B1019
-
2.7.4.1 Cupriavidus necator Q0K8Z5 i.e. Cupriavidus necator, gene ppk1 or H16_A2437
-
2.7.4.1 Cupriavidus necator Q0KA91 gene ppk2e or H16_A1979
-
2.7.4.1 Cupriavidus necator Q0KC60 gene ppk2d or H16_A1271
-
2.7.4.1 Cupriavidus necator Q0KCB8 gene ppk2c or H16_A1212
-
2.7.4.1 Cupriavidus necator Q0KCY1 gene ppk2b or H16_A0997
-
2.7.4.1 Cupriavidus necator Q0KF43 gene ppk2a or H16_A0226
-
2.7.4.1 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 Q0K2G4 gene ppk2 or ppk1b or H16_B1019
-
2.7.4.1 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 Q0K8Z5 i.e. Cupriavidus necator, gene ppk1 or H16_A2437
-
2.7.4.1 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 Q0KA91 gene ppk2e or H16_A1979
-
2.7.4.1 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 Q0KC60 gene ppk2d or H16_A1271
-
2.7.4.1 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 Q0KCB8 gene ppk2c or H16_A1212
-
2.7.4.1 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 Q0KCY1 gene ppk2b or H16_A0997
-
2.7.4.1 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 Q0KF43 gene ppk2a or H16_A0226
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ATP + (phosphate)n
-
 Cupriavidus necator ADP + (phosphate)n+1
-
 ?
2.7.4.1 ATP + (phosphate)n
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ADP + (phosphate)n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.1 ? x * 36200, about, sequence calculation Cupriavidus necator
2.7.4.1 ? x * 30700, about, sequence calculation Cupriavidus necator
2.7.4.1 ? x * 31100, about, sequence calculation Cupriavidus necator
2.7.4.1 ? x * 33900, about, sequence calculation Cupriavidus necator
2.7.4.1 ? x * 42100, about, sequence calculation Cupriavidus necator
2.7.4.1 ? x * 78200, about, sequence calculation Cupriavidus necator
2.7.4.1 ? x * 79600, about, sequence calculation Cupriavidus necator
2.7.4.1 More proteome analysis of PPK motif proteins, overview Cupriavidus necator

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 PPK1
-
 Cupriavidus necator
2.7.4.1 PPK2
-
 Cupriavidus necator
2.7.4.1 PPK2a
-
 Cupriavidus necator
2.7.4.1 PPK2B
-
 Cupriavidus necator
2.7.4.1 PPK2c
-
 Cupriavidus necator
2.7.4.1 PPK2d
-
 Cupriavidus necator
2.7.4.1 PPK2e
-
 Cupriavidus necator

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.4.1 Cupriavidus necator sequence calculation
-
 5.8
2.7.4.1 Cupriavidus necator sequence calculation
-
 5.9
2.7.4.1 Cupriavidus necator sequence calculation
-
 6.7
2.7.4.1 Cupriavidus necator sequence calculation
-
 7.8
2.7.4.1 Cupriavidus necator sequence calculation
-
 9.1
2.7.4.1 Cupriavidus necator sequence calculation
-
 9.4
2.7.4.1 Cupriavidus necator sequence calculation
-
 9.6

General Information

EC Number General Information Comment Organism
2.7.4.1 metabolism identification of a protein PhaX as a putative link between poly(3-hydroxybutyrate) and polyphospate metabolism, the A2274 (phaX) gene product is annotated as a hypothetical putative phosphate transport regulator, regulation, overview. Polyphosphate granules in Ralstonia eutropha are often located in the neighborhood of polyhydroxybutyrate granules if both biopolymers are present simultaneously Cupriavidus necator