BRENDA - Enzyme Database

High-level heterologous production of D-cycloserine by Escherichia coli

Kumagai, T.; Ozawa, T.; Tanimoto, M.; Noda, M.; Matoba, Y.; Sugiyama, M.; Appl. Environ. Microbiol. 81, 7881-7887 (2015)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
2.6.99.3
synthesis
expression of a D-cycloserine biosynthetic gene cluster consisting of 10 open reading frames (dcsA to dcsJ) from Streptomyces lavendulae ATCC 11924 in Escherichia coli. When L-serine and hydroxyurea, the precursors of D-cycloserine, are incubated together with the Escherichia coli resting cell suspension, the cells produce significant amounts of D-cycloserine (350 microM). To increase the productivity, the dcsJ gene, which might be responsible for the excretion, is connected downstream of the four genes, resulting in production of D-cycloserine at 660 microM. To repress the side catalytic activity of DcsD, i.e. the formation of L-cysteine from O-acetylserine and H2S, Escherichia coli chromosomal cysJ and cysK genes, encoding the sulfite reductase alpha subunit and O-acetylserine sulfhydrylase, respectively, are disrupted. The final strain produces 980 microM D-cycloserine
Streptomyces lavendulae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.99.3
Streptomyces lavendulae
D2Z027
-
-
2.6.99.3
Streptomyces lavendulae ATCC 11924
D2Z027
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.99.3
additional information
enzyme additionally catalyzes the formation of L-cysteine from O-acetyl-L-serine and H2S, reaction of EC 2.5.1.47
737505
Streptomyces lavendulae
?
-
-
-
-
2.6.99.3
additional information
enzyme additionally catalyzes the formation of L-cysteine from O-acetyl-L-serine and H2S, reaction of EC 2.5.1.47
737505
Streptomyces lavendulae ATCC 11924
?
-
-
-
-
2.6.99.3
O-acetyl-L-serine + hydroxyurea
-
737505
Streptomyces lavendulae
O-ureido-L-serine + acetate
-
-
-
?
2.6.99.3
O-acetyl-L-serine + hydroxyurea
-
737505
Streptomyces lavendulae ATCC 11924
O-ureido-L-serine + acetate
-
-
-
?
Application (protein specific)
EC Number
Application
Commentary
Organism
2.6.99.3
synthesis
expression of a D-cycloserine biosynthetic gene cluster consisting of 10 open reading frames (dcsA to dcsJ) from Streptomyces lavendulae ATCC 11924 in Escherichia coli. When L-serine and hydroxyurea, the precursors of D-cycloserine, are incubated together with the Escherichia coli resting cell suspension, the cells produce significant amounts of D-cycloserine (350 microM). To increase the productivity, the dcsJ gene, which might be responsible for the excretion, is connected downstream of the four genes, resulting in production of D-cycloserine at 660 microM. To repress the side catalytic activity of DcsD, i.e. the formation of L-cysteine from O-acetylserine and H2S, Escherichia coli chromosomal cysJ and cysK genes, encoding the sulfite reductase alpha subunit and O-acetylserine sulfhydrylase, respectively, are disrupted. The final strain produces 980 microM D-cycloserine
Streptomyces lavendulae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.99.3
additional information
enzyme additionally catalyzes the formation of L-cysteine from O-acetyl-L-serine and H2S, reaction of EC 2.5.1.47
737505
Streptomyces lavendulae
?
-
-
-
-
2.6.99.3
additional information
enzyme additionally catalyzes the formation of L-cysteine from O-acetyl-L-serine and H2S, reaction of EC 2.5.1.47
737505
Streptomyces lavendulae ATCC 11924
?
-
-
-
-
2.6.99.3
O-acetyl-L-serine + hydroxyurea
-
737505
Streptomyces lavendulae
O-ureido-L-serine + acetate
-
-
-
?
2.6.99.3
O-acetyl-L-serine + hydroxyurea
-
737505
Streptomyces lavendulae ATCC 11924
O-ureido-L-serine + acetate
-
-
-
?