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Literature summary extracted from

  • Kumagai, T.; Ozawa, T.; Tanimoto, M.; Noda, M.; Matoba, Y.; Sugiyama, M.
    High-level heterologous production of D-cycloserine by Escherichia coli (2015), Appl. Environ. Microbiol., 81, 7881-7887.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.6.99.3 synthesis expression of a D-cycloserine biosynthetic gene cluster consisting of 10 open reading frames (dcsA to dcsJ) from Streptomyces lavendulae ATCC 11924 in Escherichia coli. When L-serine and hydroxyurea, the precursors of D-cycloserine, are incubated together with the Escherichia coli resting cell suspension, the cells produce significant amounts of D-cycloserine (350 microM). To increase the productivity, the dcsJ gene, which might be responsible for the excretion, is connected downstream of the four genes, resulting in production of D-cycloserine at 660 microM. To repress the side catalytic activity of DcsD, i.e. the formation of L-cysteine from O-acetylserine and H2S, Escherichia coli chromosomal cysJ and cysK genes, encoding the sulfite reductase alpha subunit and O-acetylserine sulfhydrylase, respectively, are disrupted. The final strain produces 980 microM D-cycloserine Streptomyces lavendulae

Organism

EC Number Organism UniProt Comment Textmining
2.6.99.3 Streptomyces lavendulae D2Z027
-
-
2.6.99.3 Streptomyces lavendulae ATCC 11924 D2Z027
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.99.3 additional information enzyme additionally catalyzes the formation of L-cysteine from O-acetyl-L-serine and H2S, reaction of EC 2.5.1.47 Streptomyces lavendulae ?
-
 ?
2.6.99.3 additional information enzyme additionally catalyzes the formation of L-cysteine from O-acetyl-L-serine and H2S, reaction of EC 2.5.1.47 Streptomyces lavendulae ATCC 11924 ?
-
 ?
2.6.99.3 O-acetyl-L-serine + hydroxyurea
-
 Streptomyces lavendulae O-ureido-L-serine + acetate
-
 ?
2.6.99.3 O-acetyl-L-serine + hydroxyurea
-
 Streptomyces lavendulae ATCC 11924 O-ureido-L-serine + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.99.3 DcsD
-
 Streptomyces lavendulae