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Literature summary extracted from

  • De Bruyn, F.; Beauprez, J.; Maertens, J.; Soetaert, W.; De Mey, M.
    Unraveling the Leloir pathway of Bifidobacterium bifidum: significance of the uridylyltransferases (2013), Appl. Environ. Microbiol., 79, 7028-7035.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.9 expression in Escherichia coli Bifidobacterium bifidum
2.7.7.12 expression in Escherichia coli Bifidobacterium bifidum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.9 55000
-
 x * 55000, calculated Bifidobacterium bifidum
2.7.7.12 55000
-
 x * 55000, calculated Bifidobacterium bifidum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Bifidobacterium bifidum M4M6L5
-
-
2.7.7.9 Bifidobacterium bifidum PRL2010 M4M6L5
-
-
2.7.7.12 Bifidobacterium bifidum M4M7K7
-
-
2.7.7.12 Bifidobacterium bifidum M4MCS2
-
-
2.7.7.12 Bifidobacterium bifidum PRL2010 M4M7K7
-
-
2.7.7.12 Bifidobacterium bifidum PRL2010 M4MCS2
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.9 0.4
-
 substrate alpha-D-glucose 1-phosphate, pH 6.5, 37°C Bifidobacterium bifidum
2.7.7.9 13
-
 substrate alpha-D-glucose 1-phosphate, pH 6.5, 37°C Bifidobacterium bifidum
2.7.7.12 0.235
-
 substrate N-acetyl-alpha-D-glucose 1-phosphate, pH 7.0, 37°C Bifidobacterium bifidum
2.7.7.12 0.261
-
 substrate alpha-D-galactose 1-phosphate, pH 7.0, 37°C Bifidobacterium bifidum
2.7.7.12 0.334
-
 substrate N-acetyl-alpha-D-galactose 1-phosphate, pH 7.0, 37°C Bifidobacterium bifidum
2.7.7.12 93.1
-
 pH 7.0, 37°C Bifidobacterium bifidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 additional information the activity of UgpA toward glucose 1-phosphate is about 33fold higher than that toward galactose 1-phosphate Bifidobacterium bifidum ?
-
 ?
2.7.7.9 additional information the activity of UgpA toward glucose 1-phosphate is about 33fold higher than that toward galactose 1-phosphate Bifidobacterium bifidum PRL2010 ?
-
 ?
2.7.7.9 UTP + alpha-D-galactose 1-phosphate
-
 Bifidobacterium bifidum diphosphate + UDP-alpha-D-galactose
-
 ?
2.7.7.9 UTP + alpha-D-galactose 1-phosphate
-
 Bifidobacterium bifidum PRL2010 diphosphate + UDP-alpha-D-galactose
-
 ?
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Bifidobacterium bifidum diphosphate + UDP-alpha-D-glucose
-
 ?
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Bifidobacterium bifidum PRL2010 diphosphate + UDP-alpha-D-glucose
-
 ?
2.7.7.12 additional information no substrates: N-acetyl-alpha-D-galactose 1-phosphate, N-acetyl-alpha-D-glucose 1-phosphate Bifidobacterium bifidum ?
-
 ?
2.7.7.12 additional information no substrates: N-acetyl-alpha-D-galactose 1-phosphate, N-acetyl-alpha-D-glucose 1-phosphate Bifidobacterium bifidum PRL2010 ?
-
 ?
2.7.7.12 UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate
-
 Bifidobacterium bifidum alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 ?
2.7.7.12 UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate
-
 Bifidobacterium bifidum PRL2010 alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 ?
2.7.7.12 UDP-alpha-D-glucose + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 Bifidobacterium bifidum alpha-D-glucose 1-phosphate + UDP-N-acetyl-alpha-D-galactosamine
-
 ?
2.7.7.12 UDP-alpha-D-glucose + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 Bifidobacterium bifidum PRL2010 alpha-D-glucose 1-phosphate + UDP-N-acetyl-alpha-D-galactosamine
-
 ?
2.7.7.12 UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Bifidobacterium bifidum alpha-D-glucose 1-phosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 ?
2.7.7.12 UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Bifidobacterium bifidum PRL2010 alpha-D-glucose 1-phosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.9 ? x * 55000, calculated Bifidobacterium bifidum
2.7.7.12 ? x * 55000, calculated Bifidobacterium bifidum

Synonyms

EC Number Synonyms Comment Organism
2.7.7.9 UgpA
-
 Bifidobacterium bifidum
2.7.7.12 GalT1
-
 Bifidobacterium bifidum
2.7.7.12 GalT2
-
 Bifidobacterium bifidum