Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kaparullina, E.; Fedorov, D.; Doronina, N.; Trotsenko, Y.
    System of oligonucleotide primers for detection and amplification of the emoA gene encoding bacterial ethylenediaminetetraacetate monooxygenase (2008), Appl. Biochem. Microbiol., 44, 361-365.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.33 FAD stimulation Stenotrophomonas chelatiphaga
1.14.14.33 FMN stimulation Chelativorans multitrophicus

Application

EC Number Application Comment Organism
1.14.14.33 analysis design of a system of primers to obtain emoA gene fragments approximately 750 bp long for bacterial destructors of EDTA. The system can be effectively used for detecting the emoA gene in representatives of Alpha- and Gammaproteobacteria Chelativorans oligotrophicus
1.14.14.33 analysis design of a system of primers to obtain emoA gene fragments approximately 750 bp long for bacterial destructors of EDTA. The system can be effectively used for detecting the emoA gene in representatives of Alpha- and Gammaproteobacteria Chelativorans multitrophicus
1.14.14.33 analysis design of a system of primers to obtain emoA gene fragments approximately 750 bp long for bacterial destructors of EDTA. The system can be effectively used for detecting the emoA gene in representatives of Alpha- and Gammaproteobacteria Stenotrophomonas chelatiphaga

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.33 ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 Chelativorans oligotrophicus
-
 ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O overall reaction ?
1.14.14.33 ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 Chelativorans multitrophicus
-
 ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O overall reaction ?
1.14.14.33 ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 Stenotrophomonas chelatiphaga
-
 ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O overall reaction ?
1.14.14.33 ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 Chelativorans oligotrophicus LPM-4
-
 ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O overall reaction ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.33 Chelativorans multitrophicus A7LKG7
-
-
1.14.14.33 Chelativorans oligotrophicus A7LIY3
-
-
1.14.14.33 Chelativorans oligotrophicus LPM-4 A7LIY3
-
-
1.14.14.33 Stenotrophomonas chelatiphaga A7LKG8
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.33 16
-
 presence of NADH2, pH 8.0, 30°C Stenotrophomonas chelatiphaga
1.14.14.33 17
-
 presence of NADH2 and FMNH2, pH 8.0, 30°C Stenotrophomonas chelatiphaga
1.14.14.33 29
-
 presence of NADH2 and FADH2, pH 8.0, 30°C Stenotrophomonas chelatiphaga
1.14.14.33 30
-
 presence of NADH, pH 8.0, 30°C Chelativorans multitrophicus
1.14.14.33 70
-
 presence of NADH and FADH2, pH 8.0, 30°C Chelativorans multitrophicus
1.14.14.33 80
-
 presence of NADH, pH 8.0, 30°C Chelativorans oligotrophicus
1.14.14.33 81
-
 presence of NADH and FADH2, pH 8.0, 30°C Chelativorans oligotrophicus
1.14.14.33 107
-
 presence of NADH and FMNH2, pH 8.0, 30°C Chelativorans multitrophicus
1.14.14.33 156
-
 presence of NADH and FMNH2, pH 8.0, 30°C Chelativorans oligotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.33 ethylenediaminetetraacetate + 2 FADH2 + 2 O2
-
 Chelativorans oligotrophicus ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FAD + 2 H2O overall reaction ?
1.14.14.33 ethylenediaminetetraacetate + 2 FADH2 + 2 O2
-
 Chelativorans multitrophicus ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FAD + 2 H2O overall reaction ?
1.14.14.33 ethylenediaminetetraacetate + 2 FADH2 + 2 O2
-
 Stenotrophomonas chelatiphaga ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FAD + 2 H2O overall reaction ?
1.14.14.33 ethylenediaminetetraacetate + 2 FADH2 + 2 O2
-
 Chelativorans oligotrophicus LPM-4 ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FAD + 2 H2O overall reaction ?
1.14.14.33 ethylenediaminetetraacetate + 2 FMNH2 + 2 O2
-
 Chelativorans oligotrophicus ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O overall reaction ?
1.14.14.33 ethylenediaminetetraacetate + 2 FMNH2 + 2 O2
-
 Chelativorans multitrophicus ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O overall reaction ?
1.14.14.33 ethylenediaminetetraacetate + 2 FMNH2 + 2 O2
-
 Stenotrophomonas chelatiphaga ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O overall reaction ?
1.14.14.33 ethylenediaminetetraacetate + 2 FMNH2 + 2 O2
-
 Chelativorans oligotrophicus LPM-4 ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O overall reaction ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.33 EmoA
-
 Chelativorans oligotrophicus
1.14.14.33 EmoA
-
 Chelativorans multitrophicus
1.14.14.33 EmoA
-
 Stenotrophomonas chelatiphaga

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.33 FMNH2
-
 Chelativorans oligotrophicus

General Information

EC Number General Information Comment Organism
1.14.14.33 physiological function enzyme catalyzes the first reaction of EDTA decomposition by cell extracts, an NADH-dependent process that is accompanied by O2 uptake Chelativorans oligotrophicus
1.14.14.33 physiological function enzyme catalyzes the first reaction of EDTA decomposition by cell extracts, an NADH-dependent process that is accompanied by O2 uptake Chelativorans multitrophicus
1.14.14.33 physiological function enzyme catalyzes the first reaction of EDTA decomposition by cell extracts, an NADH2-dependent process that is accompanied by O2 uptake Stenotrophomonas chelatiphaga