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Literature summary extracted from

  • Jiang, C.; Song, J.; Cong, H.; Zhang, J.; Yang, Q.
    Expression and characterization of a novel antifungal exo-beta-1,3-glucanase from Chaetomium cupreum (2017), Appl. Biochem. Biotechnol., 182, 261-275.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.58 expression in Pichia pastoris Arcopilus cupreus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.58 Ag+ 5 mM, 35% residual activity Arcopilus cupreus
3.2.1.58 Co2+ 5 mM, 70% residual activity Arcopilus cupreus
3.2.1.58 Cu2+ 5 mM, 63% residual activity Arcopilus cupreus
3.2.1.58 Hg2+ 5 mM, 40% residual activity Arcopilus cupreus
3.2.1.58 Mg2+ 5 mM, 90% residual activity Arcopilus cupreus
3.2.1.58 Mn2+ 5 mM, 43% residual activity Arcopilus cupreus
3.2.1.58 additional information actvitiy is not affected by 5 mM K+, Mg2+, Ca2+, or Zn2+ Arcopilus cupreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.58 additional information
-
 Laminarin Km value 2.84 mg/ml, pH 5.0, 45°C Arcopilus cupreus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.58 65000
-
 x * 65000, SDS-PAGE Arcopilus cupreus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.58 Arcopilus cupreus
-
-
-
3.2.1.58 Arcopilus cupreus Ame
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.58 barley beta-glucan + H2O 7.4% of the activity with laminarin Arcopilus cupreus D-glucose + ?
-
 ?
3.2.1.58 barley beta-glucan + H2O 7.4% of the activity with laminarin Arcopilus cupreus Ame D-glucose + ?
-
 ?
3.2.1.58 laminaribiose + H2O
-
 Arcopilus cupreus 2 D-glucose complete hydrolysis within 2 h to yield glucose ?
3.2.1.58 laminaribiose + H2O
-
 Arcopilus cupreus Ame 2 D-glucose complete hydrolysis within 2 h to yield glucose ?
3.2.1.58 laminarin + H2O
-
 Arcopilus cupreus D-glucose + ?
-
 ?
3.2.1.58 laminarin + H2O
-
 Arcopilus cupreus Ame D-glucose + ?
-
 ?
3.2.1.58 laminaritriose + 2 H2O
-
 Arcopilus cupreus 3 D-glucose complete hydrolysis within 2 h to yield glucose ?
3.2.1.58 laminaritriose + 2 H2O
-
 Arcopilus cupreus Ame 3 D-glucose complete hydrolysis within 2 h to yield glucose ?
3.2.1.58 additional information the enzyme exhibits high substrate specificity, almost exclusively catalyzing beta-1,3-glycosidic bond cleavage in various polysaccharoses to liberate glucose. Additionally, it shows transglycosylation activity Arcopilus cupreus ?
-
 ?
3.2.1.58 additional information the enzyme exhibits high substrate specificity, almost exclusively catalyzing beta-1,3-glycosidic bond cleavage in various polysaccharoses to liberate glucose. Additionally, it shows transglycosylation activity Arcopilus cupreus Ame ?
-
 ?
3.2.1.58 zymosan A + H2O 45% of the activity with laminarin Arcopilus cupreus D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.58 ? x * 65000, SDS-PAGE Arcopilus cupreus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.58 Glu17A
-
 Arcopilus cupreus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.58 45
-
-
 Arcopilus cupreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.58 5
-
-
 Arcopilus cupreus

General Information

EC Number General Information Comment Organism
3.2.1.58 physiological function purified Glu17A exhibits antifungal activity in vitro against pathogenic fungi. Glu17A exerts the strongest enzyme activities toward the cell walls of Fusarium commune, with a value of 2.1 U/mg Arcopilus cupreus