EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.76 | expressed in Escherichia coli BL21(DE3) cells | Bacillus licheniformis |
1.1.1.304 | expression in Escherichia coli | Bacillus licheniformis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.76 | Ag+ | strong inhibition | Bacillus licheniformis | |
1.1.1.76 | Al3+ | strong inhibition | Bacillus licheniformis | |
1.1.1.76 | Cu2+ | strong inhibition | Bacillus licheniformis | |
1.1.1.76 | Fe3+ | strong inhibition | Bacillus licheniformis | |
1.1.1.76 | K+ | slight inhibition | Bacillus licheniformis | |
1.1.1.76 | additional information | not inhibited by EDTA | Bacillus licheniformis | |
1.1.1.76 | Zn2+ | slight inhibition | Bacillus licheniformis | |
1.1.1.304 | Ag+ | 1 mM, 0.5% of initial activity with substrate diacetyl, 0.5% with substrate 2,3-butanediol, respectively | Bacillus licheniformis | |
1.1.1.304 | Al3+ | 1 mM, 4% of initial activity with substrate diacetyl, 6% with substrate 2,3-butanediol, respectively | Bacillus licheniformis | |
1.1.1.304 | Cu2+ | 1 mM, 0.2% of initial activity with substrate diacetyl, 1% with substrate 2,3-butanediol, respectively | Bacillus licheniformis | |
1.1.1.304 | EDTA | 1 mM, 91% of initial activity with substrate diacetyl, 90% with substrate 2,3-butanediol, respectively | Bacillus licheniformis | |
1.1.1.304 | Fe3+ | 1 mM, 2% of initial activity with substrate diacetyl, 3.5% with substrate 2,3-butanediol, respectively | Bacillus licheniformis | |
1.1.1.304 | Zn2+ | 1 mM, 75% of initial activity with substrate diacetyl, 80% with substrate 2,3-butanediol, respectively | Bacillus licheniformis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.76 | 0.47 | - |
(2S)-acetoin | at pH 10.0 and 30°C | Bacillus licheniformis | |
1.1.1.76 | 7.25 | - |
(R,R)-butane-2,3-diol | at pH 10.0 and 30°C | Bacillus licheniformis | |
1.1.1.76 | 72.4 | - |
diacetyl | at pH 5.0 and 30°C | Bacillus licheniformis | |
1.1.1.304 | 0.25 | - |
NADH | pH 6.0, 30°C | Bacillus licheniformis | |
1.1.1.304 | 0.34 | - |
NAD+ | pH 10.0, 30°C | Bacillus licheniformis | |
1.1.1.304 | 0.47 | - |
(3S)-acetoin | pH 6.0, 30°C | Bacillus licheniformis | |
1.1.1.304 | 7.25 | - |
(2S,3S)-2,3-butanediol | pH 10.0, 30°C | Bacillus licheniformis | |
1.1.1.304 | 72.4 | - |
diacetyl | pH 6.0, 30°C | Bacillus licheniformis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.76 | Co2+ | slight activation | Bacillus licheniformis | |
1.1.1.76 | Mn2+ | slight activation | Bacillus licheniformis | |
1.1.1.76 | additional information | not influenced by Ni2+, Ba2+, Li+, Ca2+, and Na+ | Bacillus licheniformis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.76 | 30000 | - |
4 * 30000, SDS-PAGE | Bacillus licheniformis |
1.1.1.76 | 125000 | - |
gel filtration | Bacillus licheniformis |
1.1.1.304 | 30000 | - |
4 * 30000, SDS-PAGE | Bacillus licheniformis |
1.1.1.304 | 125000 | - |
gel filtration | Bacillus licheniformis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | Bacillus licheniformis | 100% activity | (2S)-acetoin + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.76 | Bacillus licheniformis | - |
- |
- |
1.1.1.304 | Bacillus licheniformis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.76 | HisTrap column chromatography and Superdex 200 gel filtration | Bacillus licheniformis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.304 | 120 | - |
substrate diacetyl, 30°C, pH 6.0 | Bacillus licheniformis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.76 | (2S)-acetoin + NADH + H+ | 97% activity compared to diacetyl | Bacillus licheniformis | (2S,3S)-butane-2,3-diol + NAD+ | - |
r | |
1.1.1.76 | (2S,3S)-butane-2,3-diol + NAD+ | 100% activity | Bacillus licheniformis | (2S)-acetoin + NADH + H+ | - |
r | |
1.1.1.76 | (R,R)-butane-2,3-diol + NAD+ | - |
Bacillus licheniformis | ? | - |
? | |
1.1.1.76 | 1,2-propanediol + NAD+ | 0.57% activity compared to (2S,3S)-butane-2,3-diol | Bacillus licheniformis | ? | - |
? | |
1.1.1.76 | 2,3-hexanedione + NADH + H+ | 66% activity compared to diacetyl | Bacillus licheniformis | ? | - |
? | |
1.1.1.76 | 2,3-pentanedione + NADH + H+ | 69% activity compared to diacetyl | Bacillus licheniformis | ? | - |
? | |
1.1.1.76 | 3,4-hexanedione + NADH + H+ | 10% activity compared to diacetyl | Bacillus licheniformis | ? | - |
? | |
1.1.1.76 | diacetyl + NADH + H+ | 100% activity | Bacillus licheniformis | (2S)-acetoin + NAD+ | - |
? | |
1.1.1.76 | additional information | the enzyme shows no activity toward racemic acetoin in the presence of NAD+ as well as no activity with NADPH, 1,4-butanediol, 2,5-hexanedione, 2,4-pentanedione, 2-butanone, methanol, mannitol, and glycerol | Bacillus licheniformis | ? | - |
? | |
1.1.1.304 | (2S,3S)-2,3-butanediol + NAD+ | - |
Bacillus licheniformis | (3S)-acetoin + NADH + H+ | - |
r | |
1.1.1.304 | (3S)-acetoin + NADH + H+ | 97% of the activity with diacetyl | Bacillus licheniformis | (2S,3S)-2,3-butanediol + NAD+ | 97.3% enantiomeric excess and 96.5% diastereomeric excess | r | |
1.1.1.304 | 1,2-propanediol + NAD+ | - |
Bacillus licheniformis | ? + NADH + H+ | 0.5% of the activity with 2,3-butanediol | r | |
1.1.1.304 | 2,3-hexanedione + NADH + H+ | - |
Bacillus licheniformis | ? + NAD+ | 66% of the activity with diacetyl | ? | |
1.1.1.304 | 2,3-pentanedione + NADH + H+ | - |
Bacillus licheniformis | ? + NAD+ | 69% of the activity with diacetyl | ? | |
1.1.1.304 | 3,4-hexanedione + NADH + H+ | - |
Bacillus licheniformis | ? + NAD+ | 10% of the activity with diacetyl | ? | |
1.1.1.304 | diacetyl + NADH + H+ | - |
Bacillus licheniformis | (3S)-acetoin + NAD+ | 97.3% enantiomeric excess | ir | |
1.1.1.304 | additional information | enzyme shows oxidative activity to racemic 2,3-butanediol but no activity toward racemic acetoin in the presence of NAD+ | Bacillus licheniformis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.76 | homotetramer | 4 * 30000, SDS-PAGE | Bacillus licheniformis |
1.1.1.304 | tetramer | 4 * 30000, SDS-PAGE | Bacillus licheniformis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.76 | BDH | - |
Bacillus licheniformis |
1.1.1.76 | budC | - |
Bacillus licheniformis |
1.1.1.76 | meso-2,3-butanediol dehydrogenase | - |
Bacillus licheniformis |
1.1.1.304 | budC | - |
Bacillus licheniformis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.76 | 37 | - |
- |
Bacillus licheniformis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.76 | 50 | - |
at 50°C, the enzymatic activity drops rapidly to half of its initial activity within 40 min, then further decreases to 20% after 330 min | Bacillus licheniformis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.76 | 202 | - |
(2S)-acetoin | at pH 10.0 and 30°C | Bacillus licheniformis | |
1.1.1.76 | 591 | - |
(R,R)-butane-2,3-diol | at pH 10.0 and 30°C | Bacillus licheniformis | |
1.1.1.76 | 1222 | - |
diacetyl | at pH 5.0 and 30°C | Bacillus licheniformis | |
1.1.1.304 | 202 | - |
(3S)-acetoin | pH 6.0, 30°C | Bacillus licheniformis | |
1.1.1.304 | 591 | - |
(2S,3S)-2,3-butanediol | pH 10.0, 30°C | Bacillus licheniformis | |
1.1.1.304 | 748 | - |
NAD+ | pH 10.0, 30°C | Bacillus licheniformis | |
1.1.1.304 | 1222 | - |
diacetyl | pH 6.0, 30°C | Bacillus licheniformis | |
1.1.1.304 | 1274 | - |
NADH | pH 6.0, 30°C | Bacillus licheniformis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.76 | 5 | - |
for diacetyl reduction | Bacillus licheniformis |
1.1.1.76 | 10 | - |
for 2,3-butanediol oxidation | Bacillus licheniformis |
1.1.1.304 | 5 | - |
reduction of diacetyl | Bacillus licheniformis |
1.1.1.304 | 10 | - |
oxidation of butanediol | Bacillus licheniformis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.76 | 4.5 | 10 | the enzyme shows a relatively high activity over a wide pH range from 5.0 to 8.0 for racemic acetoin reduction and more than 60% of activity is retained between pH 4.5 and 10.0 | Bacillus licheniformis |
1.1.1.304 | 5 | 8 | reduction of diacetyl | Bacillus licheniformis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.76 | NAD+ | - |
Bacillus licheniformis | |
1.1.1.76 | NADH | - |
Bacillus licheniformis | |
1.1.1.304 | additional information | no activity with NADPH | Bacillus licheniformis | |
1.1.1.304 | NAD+ | - |
Bacillus licheniformis | |
1.1.1.304 | NADH | - |
Bacillus licheniformis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.76 | 16.9 | - |
diacetyl | at pH 5.0 and 30°C | Bacillus licheniformis | |
1.1.1.76 | 81.5 | - |
(R,R)-butane-2,3-diol | at pH 10.0 and 30°C | Bacillus licheniformis | |
1.1.1.76 | 432 | - |
(2S)-acetoin | at pH 10.0 and 30°C | Bacillus licheniformis | |
1.1.1.304 | 16.9 | - |
diacetyl | pH 6.0, 30°C | Bacillus licheniformis | |
1.1.1.304 | 81.5 | - |
(2S,3S)-2,3-butanediol | pH 10.0, 30°C | Bacillus licheniformis | |
1.1.1.304 | 432 | - |
(3S)-acetoin | pH 6.0, 30°C | Bacillus licheniformis | |
1.1.1.304 | 2192 | - |
NAD+ | pH 10.0, 30°C | Bacillus licheniformis | |
1.1.1.304 | 5072 | - |
NADH | pH 6.0, 30°C | Bacillus licheniformis |