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Literature summary extracted from

  • Wang, Q.; Luo, Y.; He, B.; Jiang, L.S.; Liu, J.X.; Wang, J.K.
    Characterization of a novel xylanase gene from rumen content of Hu sheep (2015), Appl. Biochem. Biotechnol., 177, 1424-1436.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expression in Escherichia coli uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ag+ 10 mM, 16% residual activity uncultured bacterium
3.2.1.8 EDTA 10 mM, 72% residual activity uncultured bacterium
3.2.1.8 SDS 1%, no residual activity uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 birchwood xylan Km value 4.39 mg/ml, pH 6.0, 50°C uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Mn2+ 10 mM, 117% of initial activtiy uncultured bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 71300
-
-
 uncultured bacterium
3.2.1.8 80000
-
-
 uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 uncultured bacterium
-
 isolated from the rumen contents of Hu sheep
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 664.7
-
 pH 6.0, 50°C uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birchwood xylan + H2O
-
 uncultured bacterium xylobiose + xylose + ? xylobiose, 74% of products, plus 21% xylose ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 71300, calculated, x * 80000, SDS-PAGE uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 Xyn-Lxy
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 50
-
-
 uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 125.9
-
 birchwood xylan pH 6.0, 50°C uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6
-
-
 uncultured bacterium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 uncultured bacterium calculated
-
 5