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Literature summary extracted from

  • Yafuso, J.T.; Negi, V.S.; Bingham, J.P.; Borthakur, D.
    An O-acetylserine (thiol) lyase from Leucaena leucocephala is a cysteine synthase but not a mimosine synthase (2014), Appl. Biochem. Biotechnol., 173, 1157-1168.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.47 gene Cy-OAS-TL, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) pLYsS Leucaena leucocephala

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 additional information
-
 additional information steady-state kinetics and modeling Leucaena leucocephala
2.5.1.47 1.46
-
 hydrogen sulfide pH 7.5, 22°C, recombinant enzyme, Lineweaver-Burke model Leucaena leucocephala
2.5.1.47 1.85
-
 hydrogen sulfide pH 7.5, 22°C, recombinant enzymem, Michaelis-Menten model Leucaena leucocephala

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.47 cytosol
-
 Leucaena leucocephala 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 34000
-
-
 Leucaena leucocephala
2.5.1.47 40000
-
-
 Leucaena leucocephala

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.47 O-acetyl-L-serine + hydrogen sulfide Leucaena leucocephala
-
 L-cysteine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Leucaena leucocephala W5S3I4 gene Cy-OAS-TL
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47 recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) pLYsS by nickel affinity chhromatography Leucaena leucocephala

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 additional information no synthesis of mimosine. The enzyme is specific for cysteine synthesis. The recombinant enzyme is active with or without the leader peptide Leucaena leucocephala ?
-
 ?
2.5.1.47 O-acetyl-L-serine + hydrogen sulfide
-
 Leucaena leucocephala L-cysteine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 ? x * 40000, recombinant His-tagged enzyme, SDS-PAGE, x * 34000, about, sequence calculation of untagged enzyme Leucaena leucocephala

Synonyms

EC Number Synonyms Comment Organism
2.5.1.47 O-acetylserine (thiol) lyase
-
 Leucaena leucocephala
2.5.1.47 OAS-TL
-
 Leucaena leucocephala

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.47 22
-
 assay at room temperature Leucaena leucocephala

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 7.5
-
 assay at Leucaena leucocephala

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate
-
 Leucaena leucocephala

General Information

EC Number General Information Comment Organism
2.5.1.47 physiological function tree legume Leucaena contains a toxic, nonprotein amino acid, mimosine, which is not formed by the enzyme O-acetylserine (thiol) lyase, OAS-TL Leucaena leucocephala