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Literature summary extracted from

  • Rozova, O.N.; Khmelenina, V.N.; Gavletdinova, J.Z.; Mustakhimov, I.I.; Trotsenko, Y.A.
    Acetate kinase-an enzyme of the postulated phosphoketolase pathway in Methylomicrobium alcaliphilum 20Z (2015), Antonie van Leeuwenhoek, 108, 965-974.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.1 gene ack, DNA and amino acid sequence determination, RT-PCR analysis, transcriptional organization, phylogenetic analysis in Methylotrophic bacteria, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) Methylotuvimicrobium alcaliphilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 additional information
-
 additional information the dependence of the activity on acetate and acetyl phosphate concentrations obeys the Michaelis-Menten kinetics, whereas the dependence on ATP and ADP concentrations is sigmoidal Methylotuvimicrobium alcaliphilum
2.7.2.1 0.11
-
 acetyl phosphate pH 8.0, 30°C Methylotuvimicrobium alcaliphilum
2.7.2.1 1.28
-
 ADP pH 8.0, 30°C Methylotuvimicrobium alcaliphilum
2.7.2.1 2.36
-
 ATP pH 8.0, 30°C Methylotuvimicrobium alcaliphilum
2.7.2.1 5.6
-
 acetate pH 8.0, 30°C Methylotuvimicrobium alcaliphilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Co2+ can partly substitute for Mn2+ or Mg2+ Methylotuvimicrobium alcaliphilum
2.7.2.1 Mg2+ dependence on Mg2+ or Mn2+ ions, Km values is 0.28 mM Methylotuvimicrobium alcaliphilum
2.7.2.1 Mn2+ dependence on Mg2+ or Mn2+ ions, Km values is 0.47 mM Methylotuvimicrobium alcaliphilum
2.7.2.1 additional information no activity with Ba2+, Ca2+, Zn2+, or Cu2+ Methylotuvimicrobium alcaliphilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.1 45000
-
-
 Methylotuvimicrobium alcaliphilum
2.7.2.1 80000
-
 recombinant His6-tagged enzyme, native PAGE Methylotuvimicrobium alcaliphilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.1 ATP + acetate Methylotuvimicrobium alcaliphilum
-
 ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate Methylotuvimicrobium alcaliphilum 20Z
-
 ADP + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Methylotuvimicrobium alcaliphilum G4T0C7 gene ack
-
2.7.2.1 Methylotuvimicrobium alcaliphilum 20Z G4T0C7 gene ack
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1 recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography Methylotuvimicrobium alcaliphilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + acetate
-
 Methylotuvimicrobium alcaliphilum ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate the enzyme is 20fold more active in the reaction of acetate synthesis compared to acetate phosphorylation and shows strong specificity to ATP/ADP The enzyme has a remarkably high catalytic efficiency for acetate and ATP formation Methylotuvimicrobium alcaliphilum ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate
-
 Methylotuvimicrobium alcaliphilum 20Z ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate the enzyme is 20fold more active in the reaction of acetate synthesis compared to acetate phosphorylation and shows strong specificity to ATP/ADP The enzyme has a remarkably high catalytic efficiency for acetate and ATP formation Methylotuvimicrobium alcaliphilum 20Z ADP + acetyl phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.1 homodimer 2 * 45000, recombinant His6-tagged enzyme, SDS-PAGE Methylotuvimicrobium alcaliphilum

Synonyms

EC Number Synonyms Comment Organism
2.7.2.1 ACK
-
 Methylotuvimicrobium alcaliphilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.1 70
-
 recombinant enzyme Methylotuvimicrobium alcaliphilum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.2.1 25 80 activity range, profile overview Methylotuvimicrobium alcaliphilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.1 30 70 purified recombinant enzyme, pH 8.0, 50-70% activity is retained after a 3 h exposure at 30-70°C Methylotuvimicrobium alcaliphilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.1 1.28
-
 ADP pH 8.0, 30°C Methylotuvimicrobium alcaliphilum
2.7.2.1 2.36
-
 ATP pH 8.0, 30°C Methylotuvimicrobium alcaliphilum
2.7.2.1 233
-
 acetate pH 8.0, 30°C Methylotuvimicrobium alcaliphilum
2.7.2.1 3167
-
 acetyl phosphate pH 8.0, 30°C Methylotuvimicrobium alcaliphilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.1 8 8.5 recombinant enzyme Methylotuvimicrobium alcaliphilum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.1 6 9.5 activity range Methylotuvimicrobium alcaliphilum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.1 ADP strong specificity to ATP/ADP Methylotuvimicrobium alcaliphilum
2.7.2.1 ATP strong specificity to ATP/ADP Methylotuvimicrobium alcaliphilum
2.7.2.1 additional information no activity with CTP, GTP, or UTP Methylotuvimicrobium alcaliphilum

General Information

EC Number General Information Comment Organism
2.7.2.1 metabolism the enzyme is involved in the postulated phosphoketolase pathway Methylotuvimicrobium alcaliphilum