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Literature summary extracted from

  • Romero, I.; Tellez, J.; Romanha, A.J.; Steindel, M.; Grisard, E.C.
    Upregulation of cysteine synthase and cystathionine beta-synthase contributes to Leishmania braziliensis survival under oxidative stress (2015), Antimicrob. Agents Chemother., 59, 4770-4781.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.47 recombinant expression of the enzyme under stress conditions in Trypanosoma rangeli, a trypanosome that does not perform cysteine biosynthesis de novo, results in rescue of the cysteine synthase activity and increased rates of survival of epimastigotes expressing the enzyme compared to those of wild-type parasites Leishmania braziliensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.47 O-acetyl-L-serine + hydrogen sulfide Leishmania braziliensis
-
 L-cysteine + acetate
-
 ?
2.5.1.47 O-acetyl-L-serine + hydrogen sulfide Leishmania braziliensis MHOM/BR/75/M2904
-
 L-cysteine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Leishmania braziliensis A4HPM6
-
-
2.5.1.47 Leishmania braziliensis MHOM/BR/75/M2904 A4HPM6
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.47 amastigote intracellular Leishmania braziliensis
-
2.5.1.47 additional information quantitative enzyme expression analyis, overview Leishmania braziliensis
-
2.5.1.47 promastigote
-
 Leishmania braziliensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-serine + hydrogen sulfide
-
 Leishmania braziliensis L-cysteine + acetate
-
 ?
2.5.1.47 O-acetyl-L-serine + hydrogen sulfide
-
 Leishmania braziliensis MHOM/BR/75/M2904 L-cysteine + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.47 LbrCS
-
 Leishmania braziliensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.47 37
-
 assay at Leishmania braziliensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 7.4
-
 assay at Leishmania braziliensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate
-
 Leishmania braziliensis

Expression

EC Number Organism Comment Expression
2.5.1.47 Leishmania braziliensis the enzyme is upregulated under in vitro stress conditions induced by hydrogen peroxide, S-nitroso-N-acetylpenicillamine, or antimonial compounds in a developmental stage-specific manner up

General Information

EC Number General Information Comment Organism
2.5.1.47 physiological function upregulation of cysteine synthase and cystathionine beta-synthase contributes to Leishmania braziliensis survival under oxidative stress. Ability of Leishmania braziliensis promastigotes and amastigotes overexpressing the enzyme to resist oxidative stress, which is significantly enhanced compared to that of nontransfected cells, resulting in a phenotype far more resistant to treatment with the pentavalent form of sodium stibogluconate in vitro Leishmania braziliensis