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Literature summary extracted from

  • Thompson, S.; Zhang, Q.; Onega, M.; McMahon, S.; Fleming, I.; Ashworth, S.; Naismith, J.H.; Passchier, J.; O'Hagan, D.
    A localized tolerance in the substrate specificity of the fluorinase enzyme enables last-step 18F fluorination of a RGD peptide under ambient aqueous conditions (2014), Angew. Chem. Int. Ed. Engl., 53, 8913-8918.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.63 synthesis the enzyme is applicated in positron emission tomography (PET) as a result of its ability to catalyze C-18F bond formation in the presence of [18F]fluoride as the nucleophile. The enzymatic process has a technical advantage in the PET context because [18F]fluoride is generated in the cyclotron as a dilute solution in [18O]water, and the enzyme can use this form of aqueous fluoride directly. This feature obviates the usual requirement to secure dry [18F]fluoride by ion-exchange chromatography. The enzymatic process therefore offers the possibility to directly radiolabel biomolecules in the aqueous phase Streptomyces cattleya

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.63 purified enzyme in complex with 5'-fluorodeoxy-2-ethynyladenosine, X-ray diffraction structure determination and analysis at 2.4 A resolution, molecular replacement Streptomyces cattleya

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.63 additional information engineering of the enzyme by substrate modulation: An acetylene functionality at the C-2 position of the adenosine substrate projects from the active site into the solvent, for 18F fluorination of bioconjugated peptides by the enzyme, extending a polyethylene glycol linker from the terminus of the acetylene allows the presentation of bioconjugation cargo to the enzyme for 18F labelling, method development and evaluation, overview Streptomyces cattleya

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.63 S-adenosyl-L-methionine + fluoride Streptomyces cattleya
-
 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.63 Streptomyces cattleya Q70GK9 gene flA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.63 additional information the fluorinase catalyzes a transhalogenation of 5'-chlorodeoxy-2-ethynyladenosine to 5'-fluorodeoxy-2-ethynyladenosine, substrate specificity, the enzyme does have a specificity weakness at the C-2 position of adenine, overview Streptomyces cattleya ?
-
 ?
2.5.1.63 S-adenosyl-L-methionine + fluoride
-
 Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.63 fluorinase
-
 Streptomyces cattleya

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.63 37
-
 assay at Streptomyces cattleya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.63 8
-
 assay at Streptomyces cattleya