Immobilized Drosophila melanogaster deoxyribonucleoside kinase (DmdNK) as a high performing biocatalyst for the synthesis of purine arabinonucleotides
Serra, I.; Conti, S.; Piškur, J.; Clausen, A.; Munch-Petersen, B.; Terreni, M.; Ubiali, D.; Adv. Synth. Catal. 356, 563-570 (2014)
No PubMed abstract available
Data extracted from this reference:
Application
EC Number
Application
Commentary
Organism
2.7.1.145
synthesis
the cross-linked DmdNK preparation is used for the preparative synthesis of arabinosyladenine monophosphate (ara-AMP) and fludarabine monophosphate (FaraAMP)
Drosophila melanogaster
Engineering
EC Number
Protein Variants
Commentary
Organism
2.7.1.145
additional information
enzyme DmdNK is adsorbed on a solid ion exchange support (bearing primary amino groups) achieving an expressed activity of over 98%. Upon cross-linking with aldehyde dextran, the expressed activity is 30-40%, both biocatalysts (adsorbed or cross-linked) are stable at pH 10.0 and room temperature for 24 h with about 70% of retained activity. Optimization of the reaction conditions to 50 mM ammonium acetate, a substrate/ATP ratio of 1:1.25, 2 mM MgCl2, 37°C, pH 8.0, results in conversions of up to 90%
Drosophila melanogaster
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.145
Mg2+
required
Drosophila melanogaster
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.7.1.145
ATP + 2'-deoxyribonucleoside
Drosophila melanogaster
-
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.7.1.145
Drosophila melanogaster
Q9XZT6
gene dnk
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.7.1.145
ATP + 2'-deoxycytidine
-
737404
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
-
737404
Drosophila melanogaster
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyinosine
-
737404
Drosophila melanogaster
ADP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyribonucleoside
-
737404
Drosophila melanogaster
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
-
-
?
2.7.1.145
ATP + adenosine
low activity
737404
Drosophila melanogaster
ADP + adenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + arabinosyl-2-fluoroadenine
-
737404
Drosophila melanogaster
ADP + arabinosyl-2-fluoroadenine 5'-monophosphate
-
-
-
?
2.7.1.145
ATP + arabinosyladenine
-
737404
Drosophila melanogaster
ADP + arabinosyladenine 5'-monophosphate
-
-
-
?
2.7.1.145
ATP + arabinosyluridine
-
737404
Drosophila melanogaster
ADP + arabinosyluridine 5'-monophosphate
-
-
-
?
2.7.1.145
ATP + cytidine
-
737404
Drosophila melanogaster
ADP + cytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + guanosine
low activity
737404
Drosophila melanogaster
ADP + guanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + inosine
very low activity
737404
Drosophila melanogaster
ADP + inosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + thymidine
-
737404
Drosophila melanogaster
ADP + thymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + uridine
very low activity
737404
Drosophila melanogaster
ADP + uridine 5'-phosphate
-
-
-
?
2.7.1.145
additional information
enzyme DmdNK is able to phosphorylate all four natural 2'-deoxyribonucleosides with high efficiency, it shows broad specificity and high turnover towards the naturally occurring 2'-deoxyribonucleosides and a similar broad specificity towards nucleoside analogues used in chemotherapy, substrate specificity towards natural and non-natural nucleosides, overview
737404
Drosophila melanogaster
?
-
-
-
-
Synonyms
EC Number
Synonyms
Commentary
Organism
2.7.1.145
DmdNK
-
Drosophila melanogaster
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.145
37
-
immobilized enzyme
Drosophila melanogaster
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.145
8
-
immobilized enzyme
Drosophila melanogaster
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.145
ATP
-
Drosophila melanogaster
Application (protein specific)
EC Number
Application
Commentary
Organism
2.7.1.145
synthesis
the cross-linked DmdNK preparation is used for the preparative synthesis of arabinosyladenine monophosphate (ara-AMP) and fludarabine monophosphate (FaraAMP)
Drosophila melanogaster
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.145
ATP
-
Drosophila melanogaster
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
2.7.1.145
additional information
enzyme DmdNK is adsorbed on a solid ion exchange support (bearing primary amino groups) achieving an expressed activity of over 98%. Upon cross-linking with aldehyde dextran, the expressed activity is 30-40%, both biocatalysts (adsorbed or cross-linked) are stable at pH 10.0 and room temperature for 24 h with about 70% of retained activity. Optimization of the reaction conditions to 50 mM ammonium acetate, a substrate/ATP ratio of 1:1.25, 2 mM MgCl2, 37°C, pH 8.0, results in conversions of up to 90%
Drosophila melanogaster
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.145
Mg2+
required
Drosophila melanogaster
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.7.1.145
ATP + 2'-deoxyribonucleoside
Drosophila melanogaster
-
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.7.1.145
ATP + 2'-deoxycytidine
-
737404
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyguanosine
-
737404
Drosophila melanogaster
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyinosine
-
737404
Drosophila melanogaster
ADP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + 2'-deoxyribonucleoside
-
737404
Drosophila melanogaster
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
-
-
?
2.7.1.145
ATP + adenosine
low activity
737404
Drosophila melanogaster
ADP + adenosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + arabinosyl-2-fluoroadenine
-
737404
Drosophila melanogaster
ADP + arabinosyl-2-fluoroadenine 5'-monophosphate
-
-
-
?
2.7.1.145
ATP + arabinosyladenine
-
737404
Drosophila melanogaster
ADP + arabinosyladenine 5'-monophosphate
-
-
-
?
2.7.1.145
ATP + arabinosyluridine
-
737404
Drosophila melanogaster
ADP + arabinosyluridine 5'-monophosphate
-
-
-
?
2.7.1.145
ATP + cytidine
-
737404
Drosophila melanogaster
ADP + cytidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + guanosine
low activity
737404
Drosophila melanogaster
ADP + guanosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + inosine
very low activity
737404
Drosophila melanogaster
ADP + inosine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + thymidine
-
737404
Drosophila melanogaster
ADP + thymidine 5'-phosphate
-
-
-
?
2.7.1.145
ATP + uridine
very low activity
737404
Drosophila melanogaster
ADP + uridine 5'-phosphate
-
-
-
?
2.7.1.145
additional information
enzyme DmdNK is able to phosphorylate all four natural 2'-deoxyribonucleosides with high efficiency, it shows broad specificity and high turnover towards the naturally occurring 2'-deoxyribonucleosides and a similar broad specificity towards nucleoside analogues used in chemotherapy, substrate specificity towards natural and non-natural nucleosides, overview
737404
Drosophila melanogaster
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.145
37
-
immobilized enzyme
Drosophila melanogaster
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.145
8
-
immobilized enzyme
Drosophila melanogaster