EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.1.145 | synthesis | the cross-linked DmdNK preparation is used for the preparative synthesis of arabinosyladenine monophosphate (ara-AMP) and fludarabine monophosphate (FaraAMP) | Drosophila melanogaster |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.145 | additional information | enzyme DmdNK is adsorbed on a solid ion exchange support (bearing primary amino groups) achieving an expressed activity of over 98%. Upon cross-linking with aldehyde dextran, the expressed activity is 30-40%, both biocatalysts (adsorbed or cross-linked) are stable at pH 10.0 and room temperature for 24 h with about 70% of retained activity. Optimization of the reaction conditions to 50 mM ammonium acetate, a substrate/ATP ratio of 1:1.25, 2 mM MgCl2, 37°C, pH 8.0, results in conversions of up to 90% | Drosophila melanogaster |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.145 | Mg2+ | required | Drosophila melanogaster |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.145 | ATP + 2'-deoxyribonucleoside | Drosophila melanogaster | - |
ADP + 2'-deoxyribonucleoside 5'-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.145 | Drosophila melanogaster | Q9XZT6 | gene dnk | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.145 | ATP + 2'-deoxycytidine | - |
Drosophila melanogaster | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxyguanosine | - |
Drosophila melanogaster | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxyinosine | - |
Drosophila melanogaster | ADP + 2'-deoxyinosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxyribonucleoside | - |
Drosophila melanogaster | ADP + 2'-deoxyribonucleoside 5'-phosphate | - |
? | |
2.7.1.145 | ATP + adenosine | low activity | Drosophila melanogaster | ADP + adenosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + arabinosyl-2-fluoroadenine | - |
Drosophila melanogaster | ADP + arabinosyl-2-fluoroadenine 5'-monophosphate | - |
? | |
2.7.1.145 | ATP + arabinosyladenine | - |
Drosophila melanogaster | ADP + arabinosyladenine 5'-monophosphate | - |
? | |
2.7.1.145 | ATP + arabinosyluridine | - |
Drosophila melanogaster | ADP + arabinosyluridine 5'-monophosphate | - |
? | |
2.7.1.145 | ATP + cytidine | - |
Drosophila melanogaster | ADP + cytidine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + guanosine | low activity | Drosophila melanogaster | ADP + guanosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + inosine | very low activity | Drosophila melanogaster | ADP + inosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + thymidine | - |
Drosophila melanogaster | ADP + thymidine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + uridine | very low activity | Drosophila melanogaster | ADP + uridine 5'-phosphate | - |
? | |
2.7.1.145 | additional information | enzyme DmdNK is able to phosphorylate all four natural 2'-deoxyribonucleosides with high efficiency, it shows broad specificity and high turnover towards the naturally occurring 2'-deoxyribonucleosides and a similar broad specificity towards nucleoside analogues used in chemotherapy, substrate specificity towards natural and non-natural nucleosides, overview | Drosophila melanogaster | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.145 | DmdNK | - |
Drosophila melanogaster |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.145 | 37 | - |
immobilized enzyme | Drosophila melanogaster |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.145 | 8 | - |
immobilized enzyme | Drosophila melanogaster |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.145 | ATP | - |
Drosophila melanogaster |