EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.4.9 | expression in Escherichia coli | Wolbachia endosymbiont of Brugia malayi |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.9 | 3'-azido-3'-deoxythymidine | specific inhibitory effect on the enzyme activity, and these effect is in good association with binding interactions and the scoring functions | Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | 5-bromo-2'-deoxyuridine | specific inhibitory effect on the enzyme activity, and these effect is in good association with binding interactions and the scoring functions | Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | 5-chloro-2'-deoxyuridine | specific inhibitory effect on the enzyme activity, and these effect is in good association with binding interactions and the scoring functions | Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | albendazole | - |
Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | ATP | - |
Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | diethylcarbamazine | - |
Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | dTDP | competitive with dTMP | Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | ivermectin | - |
Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | levamisole | - |
Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | suramin | - |
Wolbachia endosymbiont of Brugia malayi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.9 | 0.017 | - |
dTMP | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | 0.066 | - |
ATP | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.9 | Co2+ | may substitute for Mg2+ | Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | Mg2+ | most favourable divalent cation | Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | Mn2+ | 52% of the activtiy with Mg2+ | Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | additional information | presence of a divalent cation is required. In presence of Ca2+, Cu2+, less than 10% of maximum activity, no activity with Zn2+ | Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | Ni2+ | 10% of the activity with Mg2+ | Wolbachia endosymbiont of Brugia malayi |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.4.9 | 24000 | - |
2 * 24000, SDS-PAGE | Wolbachia endosymbiont of Brugia malayi |
2.7.4.9 | 52000 | - |
gel filtration | Wolbachia endosymbiont of Brugia malayi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.9 | Wolbachia endosymbiont of Brugia malayi | Q5GTD5 | - |
- |
2.7.4.9 | Wolbachia endosymbiont of Brugia malayi TRS | Q5GTD5 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.9 | ATP + dTMP | - |
Wolbachia endosymbiont of Brugia malayi | ADP + dTDP | - |
? | |
2.7.4.9 | ATP + dTMP | - |
Wolbachia endosymbiont of Brugia malayi TRS | ADP + dTDP | - |
? | |
2.7.4.9 | ATP + dUMP | - |
Wolbachia endosymbiont of Brugia malayi | ADP + dUDP | 12% of the activity with dTMP | ? | |
2.7.4.9 | ATP + dUMP | - |
Wolbachia endosymbiont of Brugia malayi TRS | ADP + dUDP | 12% of the activity with dTMP | ? | |
2.7.4.9 | dATP + dTMP | 26.9% of the activity with ATP | Wolbachia endosymbiont of Brugia malayi | dADP + dTDP | - |
? | |
2.7.4.9 | dATP + dTMP | 26.9% of the activity with ATP | Wolbachia endosymbiont of Brugia malayi TRS | dADP + dTDP | - |
? | |
2.7.4.9 | additional information | maximal activity is achieved at equimolar concentrations of ATP and Mg2+ | Wolbachia endosymbiont of Brugia malayi | ? | - |
? | |
2.7.4.9 | additional information | maximal activity is achieved at equimolar concentrations of ATP and Mg2+ | Wolbachia endosymbiont of Brugia malayi TRS | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.4.9 | dimer | 2 * 24000, SDS-PAGE | Wolbachia endosymbiont of Brugia malayi |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.4.9 | TMK | - |
Wolbachia endosymbiont of Brugia malayi |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.4.9 | 37 | - |
- |
Wolbachia endosymbiont of Brugia malayi |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.4.9 | 45 | - |
30% residual activity | Wolbachia endosymbiont of Brugia malayi |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.4.9 | 40 | - |
stable below | Wolbachia endosymbiont of Brugia malayi |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.9 | 38.1 | - |
ATP | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.4.9 | 7.4 | - |
- |
Wolbachia endosymbiont of Brugia malayi |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.4.9 | 7 | 8 | marked loss of activity above or below | Wolbachia endosymbiont of Brugia malayi |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.9 | 0.02 | - |
5-chloro-2'-deoxyuridine | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | 0.024 | - |
3'-azido-3'-deoxythymidine | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | 0.042 | - |
5-bromo-2'-deoxyuridine | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | 0.21 | - |
ATP | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | 0.23 | - |
dTDP | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.7.4.9 | 0.018 | - |
pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | suramin | |
2.7.4.9 | 0.14 | - |
pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | albendazole | |
2.7.4.9 | 0.15 | - |
pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | ivermectin | |
2.7.4.9 | 0.75 | - |
pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | diethylcarbamazine | |
2.7.4.9 | 1.2 | - |
pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | levamisole |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.9 | 577 | - |
ATP | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | |
2.7.4.9 | 2240 | - |
dTMP | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi |