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Literature summary extracted from

  • Doharey, P.K.; Suthar, M.K.; Verma, A.; Kumar, V.; Yadav, S.; Balaramnavar, V.M.; Rathaur, S.; Saxena, A.K.; Siddiqi, M.I.; Saxena, J.K.
    Molecular cloning and characterization of Brugia malayi thymidylate kinase (2014), Acta Trop., 133, 83-92.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.9 expression in Escherichia coli Wolbachia endosymbiont of Brugia malayi

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.9 3'-azido-3'-deoxythymidine specific inhibitory effect on the enzyme activity, and these effect is in good association with binding interactions and the scoring functions Wolbachia endosymbiont of Brugia malayi
2.7.4.9 5-bromo-2'-deoxyuridine specific inhibitory effect on the enzyme activity, and these effect is in good association with binding interactions and the scoring functions Wolbachia endosymbiont of Brugia malayi
2.7.4.9 5-chloro-2'-deoxyuridine specific inhibitory effect on the enzyme activity, and these effect is in good association with binding interactions and the scoring functions Wolbachia endosymbiont of Brugia malayi
2.7.4.9 albendazole
-
Wolbachia endosymbiont of Brugia malayi
2.7.4.9 ATP
-
Wolbachia endosymbiont of Brugia malayi
2.7.4.9 diethylcarbamazine
-
Wolbachia endosymbiont of Brugia malayi
2.7.4.9 dTDP competitive with dTMP Wolbachia endosymbiont of Brugia malayi
2.7.4.9 ivermectin
-
Wolbachia endosymbiont of Brugia malayi
2.7.4.9 levamisole
-
Wolbachia endosymbiont of Brugia malayi
2.7.4.9 suramin
-
Wolbachia endosymbiont of Brugia malayi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.9 0.017
-
dTMP pH 7.4, temperature not specified in the publication Wolbachia endosymbiont of Brugia malayi
2.7.4.9 0.066
-
ATP pH 7.4, temperature not specified in the publication Wolbachia endosymbiont of Brugia malayi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.9 Co2+ may substitute for Mg2+ Wolbachia endosymbiont of Brugia malayi
2.7.4.9 Mg2+ most favourable divalent cation Wolbachia endosymbiont of Brugia malayi
2.7.4.9 Mn2+ 52% of the activtiy with Mg2+ Wolbachia endosymbiont of Brugia malayi
2.7.4.9 additional information presence of a divalent cation is required. In presence of Ca2+, Cu2+, less than 10% of maximum activity, no activity with Zn2+ Wolbachia endosymbiont of Brugia malayi
2.7.4.9 Ni2+ 10% of the activity with Mg2+ Wolbachia endosymbiont of Brugia malayi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.9 24000
-
2 * 24000, SDS-PAGE Wolbachia endosymbiont of Brugia malayi
2.7.4.9 52000
-
gel filtration Wolbachia endosymbiont of Brugia malayi

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.9 Wolbachia endosymbiont of Brugia malayi Q5GTD5
-
-
2.7.4.9 Wolbachia endosymbiont of Brugia malayi TRS Q5GTD5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.9 ATP + dTMP
-
Wolbachia endosymbiont of Brugia malayi ADP + dTDP
-
?
2.7.4.9 ATP + dTMP
-
Wolbachia endosymbiont of Brugia malayi TRS ADP + dTDP
-
?
2.7.4.9 ATP + dUMP
-
Wolbachia endosymbiont of Brugia malayi ADP + dUDP 12% of the activity with dTMP ?
2.7.4.9 ATP + dUMP
-
Wolbachia endosymbiont of Brugia malayi TRS ADP + dUDP 12% of the activity with dTMP ?
2.7.4.9 dATP + dTMP 26.9% of the activity with ATP Wolbachia endosymbiont of Brugia malayi dADP + dTDP
-
?
2.7.4.9 dATP + dTMP 26.9% of the activity with ATP Wolbachia endosymbiont of Brugia malayi TRS dADP + dTDP
-
?
2.7.4.9 additional information maximal activity is achieved at equimolar concentrations of ATP and Mg2+ Wolbachia endosymbiont of Brugia malayi ?
-
?
2.7.4.9 additional information maximal activity is achieved at equimolar concentrations of ATP and Mg2+ Wolbachia endosymbiont of Brugia malayi TRS ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.4.9 dimer 2 * 24000, SDS-PAGE Wolbachia endosymbiont of Brugia malayi

Synonyms

EC Number Synonyms Comment Organism
2.7.4.9 TMK
-
Wolbachia endosymbiont of Brugia malayi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.9 37
-
-
Wolbachia endosymbiont of Brugia malayi

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.4.9 45
-
30% residual activity Wolbachia endosymbiont of Brugia malayi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.9 40
-
stable below Wolbachia endosymbiont of Brugia malayi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.9 38.1
-
ATP pH 7.4, temperature not specified in the publication Wolbachia endosymbiont of Brugia malayi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.9 7.4
-
-
Wolbachia endosymbiont of Brugia malayi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.9 7 8 marked loss of activity above or below Wolbachia endosymbiont of Brugia malayi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.9 0.02
-
5-chloro-2'-deoxyuridine pH 7.4, temperature not specified in the publication Wolbachia endosymbiont of Brugia malayi
2.7.4.9 0.024
-
3'-azido-3'-deoxythymidine pH 7.4, temperature not specified in the publication Wolbachia endosymbiont of Brugia malayi
2.7.4.9 0.042
-
5-bromo-2'-deoxyuridine pH 7.4, temperature not specified in the publication Wolbachia endosymbiont of Brugia malayi
2.7.4.9 0.21
-
ATP pH 7.4, temperature not specified in the publication Wolbachia endosymbiont of Brugia malayi
2.7.4.9 0.23
-
dTDP pH 7.4, temperature not specified in the publication Wolbachia endosymbiont of Brugia malayi

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.4.9 0.018
-
pH 7.4, temperature not specified in the publication Wolbachia endosymbiont of Brugia malayi suramin
2.7.4.9 0.14
-
pH 7.4, temperature not specified in the publication Wolbachia endosymbiont of Brugia malayi albendazole
2.7.4.9 0.15
-
pH 7.4, temperature not specified in the publication Wolbachia endosymbiont of Brugia malayi ivermectin
2.7.4.9 0.75
-
pH 7.4, temperature not specified in the publication Wolbachia endosymbiont of Brugia malayi diethylcarbamazine
2.7.4.9 1.2
-
pH 7.4, temperature not specified in the publication Wolbachia endosymbiont of Brugia malayi levamisole

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.4.9 577
-
ATP pH 7.4, temperature not specified in the publication Wolbachia endosymbiont of Brugia malayi
2.7.4.9 2240
-
dTMP pH 7.4, temperature not specified in the publication Wolbachia endosymbiont of Brugia malayi