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Literature summary extracted from

  • Sutton, K.A.; Breen, J.; Russo, T.A.; Schultz, L.W.; Umland, T.C.
    Crystal structure of 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase from the ESKAPE pathogen Acinetobacter baumannii (2016), Acta Crystallogr. Sect. F, 72, 179-187.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.19 expressed in Escherichia coli Rosetta (DE3) cells Acinetobacter baumannii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.19 microbatch under oil method, using 100 mM bis-Tris propane pH 7.0, 100 mM potassium bromide, 40% (w/v) PEG 8000 Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Acinetobacter baumannii
-
 phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Acinetobacter baumannii 307-0294
-
 phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Acinetobacter baumannii
-
-
-
2.5.1.19 Acinetobacter baumannii 307-0294
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.19 HiTrap column chromatography and Superdex 200 gel filtration Acinetobacter baumannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Acinetobacter baumannii phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Acinetobacter baumannii 307-0294 phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.19 5-enol-pyruvylshikimate-3-phosphate synthase
-
 Acinetobacter baumannii
2.5.1.19 AroA
-
 Acinetobacter baumannii
2.5.1.19 EPSP synthase
-
 Acinetobacter baumannii