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Literature summary extracted from

  • Ho, T.H.; Hong, M.K.; Ngo, H.P.; Kang, L.W.
    Expression, crystallization and preliminary X-ray crystallographic analysis of DNA-directed RNA polymerase subunit L from Thermococcus onnurineus NA1 (2014), Acta Crystallogr. Sect. F, 70, 639-642.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.6 gene TON-0309 encoding DNA-directed RNA polymerase subunit L, recombinant overexpression of subunit L in Escherichia coli strain BL21(DE3) Thermococcus onnurineus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.6 purified recombinant DNA-directed RNA polymerase subunit L, hanging drop vapour diffusion method, mixing of 15 mg/ml protein in a buffer consisting of 25 mM Tris-HCl, pH 7.5, 15 mM NaCl, 3 mM 2-mercaptoethanol, with 500 nl reservoir solution containing 0.2 M lithium sulfate, 21% w/v PEG 3350, 15% v/v MPD, 0.1 M imidazole-HCl, pH 6.0, and equilibration against 0.07 ml of reservoir solution, 10-14°C, X-ray diffraction structure determination and analysis at 2.1 A resolution Thermococcus onnurineus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn Thermococcus onnurineus
-
 diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Thermococcus onnurineus B6YTA7 gene TON-0309
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6 recombinant RNAP subunit L from Escherichia coli strain BL21(DE3) by anion exchange chromatography and ultrafiltration Thermococcus onnurineus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn
-
 Thermococcus onnurineus diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.6 RNA polymerase
-
 Thermococcus onnurineus
2.7.7.6 RNAP
-
 Thermococcus onnurineus

General Information

EC Number General Information Comment Organism
2.7.7.6 physiological function RNA polymerase plays a crucial role in gene expression in all organisms. It is a multiprotein complex that produces primary transcript RNA from a DNA template Thermococcus onnurineus