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Literature summary extracted from

  • Faim, L.M.; Rosa e Silva, I.; Bertacine Dias, M.V.; DMuniz Pereira, H.; Brandao-Neto, J.; Alves da Silva, M.T.; Thiemann, O.H.
    Crystallization and preliminary X-ray diffraction analysis of selenophosphate synthetases from Trypanosoma brucei and Leishmania major (2013), Acta Crystallogr. Sect. F, 69, 864-867.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.3 recombinant expression of His6-tagged enzyme with a thrombin-cleavage site in Escherichia coli strain BL21(DE3) Leishmania major
2.7.9.3 recombinant expression of His6-tagged enzyme with a thrombin-cleavage site in Escherichia coli strain BL21(DE3) Trypanosoma brucei brucei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.9.3 recombinant His6-tagged enzyme, from 0.1 M trisodium citrate, pH 5.6, 17% w/v, PEG 3350 using the microbatch method, 2 months, X-ray diffraction structure determination and analysis at 3.40 A resolution Trypanosoma brucei brucei
2.7.9.3 the recombinant full-length enzyme cannot be crystallized, but the DELTAN-LmSPS2, which lacks residues 1-69, is efficiently crystallized, from 0.2 M ammonium sulfate, 0.1 M trisodium citrate, pH 5.2, and 21% w/v PEG 4000, 3 days, X-ray diffraction structure determination and analysis at 1.88 A resolution Leishmania major

Protein Variants

EC Number Protein Variants Comment Organism
2.7.9.3 additional information construction of an N-terminal truncation mtant enzyme lacking the first 69 amino acids, DELTAN-LmSPS2 Leishmania major

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.3 Mg2+ required Leishmania major
2.7.9.3 Mg2+ required Trypanosoma brucei brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.9.3 44700
-
-
 Leishmania major
2.7.9.3 45000
-
-
 Trypanosoma brucei brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.3 ATP + selenide + H2O Leishmania major
-
 AMP + selenophosphate + phosphate
-
 ?
2.7.9.3 ATP + selenide + H2O Trypanosoma brucei brucei
-
 AMP + selenophosphate + phosphate
-
 ?
2.7.9.3 ATP + selenide + H2O Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927
-
 AMP + selenophosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.3 Leishmania major Q4Q0M0
-
-
2.7.9.3 Trypanosoma brucei brucei Q38A34
-
-
2.7.9.3 Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 Q38A34
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.3 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, and gel filtration Leishmania major
2.7.9.3 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, and gel filtration Trypanosoma brucei brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.3 ATP + selenide + H2O
-
 Leishmania major AMP + selenophosphate + phosphate
-
 ?
2.7.9.3 ATP + selenide + H2O
-
 Trypanosoma brucei brucei AMP + selenophosphate + phosphate
-
 ?
2.7.9.3 ATP + selenide + H2O
-
 Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 AMP + selenophosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.9.3 ? x * 44700, recombinant His6-tagged enzyme, SDS-PAGE Leishmania major
2.7.9.3 ? x * 45000, recombinant His6-tagged enzyme, SDS-PAGE Trypanosoma brucei brucei

Synonyms

EC Number Synonyms Comment Organism
2.7.9.3 LmSPS2
-
 Leishmania major
2.7.9.3 selenophosphate synthetase
-
 Leishmania major
2.7.9.3 selenophosphate synthetase
-
 Trypanosoma brucei brucei
2.7.9.3 SPS
-
 Leishmania major
2.7.9.3 SPS
-
 Trypanosoma brucei brucei
2.7.9.3 TbSPS2
-
 Trypanosoma brucei brucei

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.9.3 ATP
-
 Leishmania major
2.7.9.3 ATP
-
 Trypanosoma brucei brucei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.9.3 Leishmania major isoelectric focusing, recombinant His6-tagged enzyme
-
 5.83
2.7.9.3 Trypanosoma brucei brucei isoelectric focusing, recombinant His6-tagged enzyme
-
 5.98

General Information

EC Number General Information Comment Organism
2.7.9.3 physiological function the enzyme plays an indispensable role in selenium metabolism, being responsible for catalyzing the activation of selenide with adenosine 5'-triphosphate (ATP) to generate selenophosphate, the essential selenium donor for selenocysteine synthesis Trypanosoma brucei brucei