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Literature summary extracted from

  • Amano, Y.; Namatame, I.; Tateishi, Y.; Honboh, K.; Tanabe, E.; Niimi, T.; Sakashita, H.
    Structural insights into the novel inhibition mechanism of Trypanosoma cruzi spermidine synthase (2015), Acta Crystallogr. Sect. D, 71, 1879-1889.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.16 expressed in Escherichia coli BL21(DE3) cells Trypanosoma cruzi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.16 in complex with decarboxylated S-adenosylmethionine, trans-4-methylcyclohexylamine and inhibitors, sitting drop vapor diffusion method, using 100 mM bis-Tris pH 5.5-6.5, 200 mM ammonium sulfate, 10-15% (w/v) PEG 4000 Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.16 2-phenyl-1,2-thiazol-3(2H)-one
-
 Trypanosoma cruzi
2.5.1.16 2H-1,4-benzothiazin-3-amine
-
 Trypanosoma cruzi
2.5.1.16 5-[(4-methylbenzyl)oxy]quinazolin-2-amine
-
 Trypanosoma cruzi
2.5.1.16 isoquinolin-1-amine
-
 Trypanosoma cruzi
2.5.1.16 N-methyl-N-phenyl-2,3-dihydro-1,4-benzodioxine-2-carboxamide
-
 Trypanosoma cruzi
2.5.1.16 quinolin-8-yl piperidine-1-carboxylate
-
 Trypanosoma cruzi
2.5.1.16 S-adenosyl-1,8-diamino-3-thio-octane
-
 Trypanosoma cruzi
2.5.1.16 trans-4-Methylcyclohexylamine
-
 Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.16 S-adenosyl 3-(methylthio)propylamine + putrescine Trypanosoma cruzi
-
 S-methyl-5'-thioadenosine + spermidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Trypanosoma cruzi Q4DA73
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16 Ni-NTA column chromatography and Superdex 75 gel filtration Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 S-adenosyl 3-(methylthio)propylamine + putrescine
-
 Trypanosoma cruzi S-methyl-5'-thioadenosine + spermidine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.16 homodimer
-
 Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
2.5.1.16 SPDSYN
-
 Trypanosoma cruzi

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.16 0.000051
-
 pH and temperature not specified in the publication Trypanosoma cruzi 2-phenyl-1,2-thiazol-3(2H)-one
2.5.1.16 0.0091
-
 pH and temperature not specified in the publication Trypanosoma cruzi 5-[(4-methylbenzyl)oxy]quinazolin-2-amine
2.5.1.16 0.18
-
 pH and temperature not specified in the publication Trypanosoma cruzi 2H-1,4-benzothiazin-3-amine
2.5.1.16 0.46
-
 pH and temperature not specified in the publication Trypanosoma cruzi isoquinolin-1-amine
2.5.1.16 0.53
-
 pH and temperature not specified in the publication Trypanosoma cruzi N-methyl-N-phenyl-2,3-dihydro-1,4-benzodioxine-2-carboxamide
2.5.1.16 0.83
-
 pH and temperature not specified in the publication Trypanosoma cruzi quinolin-8-yl piperidine-1-carboxylate