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Literature summary extracted from

  • Sutton, K.A.; Breen, J.; MacDonald, U.; Beanan, J.M.; Olson, R.; Russo, T.A.; Schultz, L.W.; Umland, T.C.
    Structure of shikimate kinase, an in vivo essential metabolic enzyme in the nosocomial pathogen Acinetobacter baumannii, in complex with shikimate (2015), Acta Crystallogr. Sect. D, 71, 1736-1744.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.71 expressed in Escherichia coli Rosetta (DE3) cells Acinetobacter baumannii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.71 in complex with shikimate, microbatch-under-oil method and sitting-drop vapor diffusion method using 0.1 M HEPES pH 7.5, 1.5 M lithium sulfate Acinetobacter baumannii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.71 shikimate substrate inhibition Acinetobacter baumannii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.71 0.147
-
 shikimate at pH 7.5 and 25°C Acinetobacter baumannii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.71 Mg2+ required Acinetobacter baumannii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.71 21500
-
 gel filtration Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.71 ATP + shikimate Acinetobacter baumannii
-
 ADP + 3-phosphoshikimate
-
 ?
2.7.1.71 ATP + shikimate Acinetobacter baumannii 307-0294
-
 ADP + 3-phosphoshikimate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.71 Acinetobacter baumannii A0A0M3KL09
-
-
2.7.1.71 Acinetobacter baumannii 307-0294 A0A0M3KL09
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.71 HisTrap column chromatography, and Superdex 75 gel filtration Acinetobacter baumannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.71 ATP + shikimate
-
 Acinetobacter baumannii ADP + 3-phosphoshikimate
-
 ?
2.7.1.71 ATP + shikimate
-
 Acinetobacter baumannii 307-0294 ADP + 3-phosphoshikimate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.71 monomer 1 * 21500, SDS-PAGE Acinetobacter baumannii

Synonyms

EC Number Synonyms Comment Organism
2.7.1.71 AroK
-
 Acinetobacter baumannii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.71 21.4
-
 shikimate at pH 7.5 and 25°C Acinetobacter baumannii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.71 4.95
-
 shikimate at pH 7.5 and 25°C Acinetobacter baumannii