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Literature summary extracted from

  • Serer, M.I.; Bonomi, H.R.; Guimaraes, B.G.; Rossi, R.C.; Goldbaum, F.A.; Klinke, S.
    Crystallographic and kinetic study of riboflavin synthase from Brucella abortus, a chemotherapeutic target with an enhanced intrinsic flexibility (2014), Acta Crystallogr. Sect. D, 70, 1419-1434.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.9 expressed in Escherichia coli BL21(DE3) cells Brucella abortus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.9 hanging drop vapor diffusion method, using 12% (w/v) PEG 8000, 10% (w/v) glycerol, 0.5 M potassium chloride Brucella abortus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.9 66000
-
-
 Brucella abortus
2.5.1.9 69800
-
-
 Brucella abortus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.9 6,7-dimethyl-8-(1-D-ribityl)lumazine Brucella abortus
-
 riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-(1-D-ribityl)lumazine Brucella abortus 2308
-
 riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.9 Brucella abortus Q2YN92
-
-
2.5.1.9 Brucella abortus 2308 Q2YN92
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.9 HisTrap column chromatography and Superdex 200 gel filtration Brucella abortus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.9 6,7-dimethyl-8-(1-D-ribityl)lumazine
-
 Brucella abortus riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
2.5.1.9 6,7-dimethyl-8-(1-D-ribityl)lumazine
-
 Brucella abortus 2308 riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.9 homotrimer 3 * 66000, static light scattering Brucella abortus
2.5.1.9 homotrimer 3 * 69800, calculated from amino acid sequence Brucella abortus