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Literature summary extracted from

  • Hitchcock, A.; Jackson, P.J.; Chidgey, J.W.; Dickman, M.J.; Hunter, C.N.; Canniffe, D.P.
    Biosynthesis of chlorophyll a in a purple bacterial phototroph and assembly into a plant chlorophyll-protein complex (2016), ACS Synth. Biol., 5, 948-954.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.62 gene chlG, functional recombinant expression of codon-optimizes Synechocystis sp. gene chlG with Synechocystis sp. gene chlP in Rhodobacter sphaeroides lacking genes bchlG and bchlP, mutant subcloning in Escherichia coli strains JM109 and S17-1 Synechocystis sp. PCC 6803

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.B43 additional information improvements to photosynthetic efficiency can be achieved by manipulating pigment biosynthetic pathways of photosynthetic organisms in order to increase the spectral coverage for light absorption via development of organisms that can produce both bacteriochlorophylls and chlorophylls, engineering of the bacteriochlorophyll-utilizing anoxygenic phototroph Rhodobacter sphaeroides to make chlorophyll a. Deletion of genes responsible for the bacteriochlorophyll-specific modifications of chlorophyllide and replacement of the native bacteriochlorophyll synthase with a cyanobacterial chlorophyll synthase results in the production of chlorophyll a. Chlorophyll a can be assembled in vivo into the plant water-soluble chlorophyll protein, heterologously produced in Rhodobacter sphaeroides, method optimization, overview. Mutant subcloning in Escherichia coli strains JM109 and S17-1 Cereibacter sphaeroides
2.5.1.62 additional information improvements to photosynthetic efficiency could be achieved by manipulating pigment biosynthetic pathways of photosynthetic organisms in order to increase the spectral coverage for light absorption via development of organisms that can produce both bacteriochlorophylls and chlorophylls, engineering of the bacteriochlorophyll-utilizing anoxygenic phototroph Rhodobacter sphaeroides to make chlorophyll a. Deletion of genes responsible for the bacteriochlorophyll-specific modifications of chlorophyllide and replacement of the native bacteriochlorophyll synthase with a cyanobacterial chlorophyll synthase results in the production of chlorophyll a. Chlorophyll a can be assembled in vivo into the plant water-soluble chlorophyll protein, heterologously produced in Rhodobacter sphaeroides, method optimization, overview Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.B43 bacteriochlorophyllide a + phytyl diphosphate Cereibacter sphaeroides
-
 bacteriochlorophyll a + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.B43 Cereibacter sphaeroides A4WRA3
-
-
2.5.1.62 Synechocystis sp. PCC 6803 Q55145 gene chlG
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.B43 phytyl diphosphate + bacteriochlorophyllide a = bacteriochlorophyll a + diphosphate the enzyme catalyses the direct addition of a phytyl chain to bacteriochlorophyllide a via an ester bond with the 17-propionate residue resulting in bacteriochlorophyll a Cereibacter sphaeroides
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.B43 bacteriochlorophyllide a + phytyl diphosphate
-
 Cereibacter sphaeroides bacteriochlorophyll a + diphosphate
-
 ?
2.5.1.B43 additional information an engineered enzyme mutant is also active with chlorophyllide a and phytyl diphosphate producing chlorophyll a and diphosphate, cf. EC 2.5.1.62 Cereibacter sphaeroides ?
-
 ?
2.5.1.62 chlorophyllide a + phytyl diphosphate engineered enzyme mutant Synechocystis sp. PCC 6803 chlorophyll a + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.62 ChlG
-
 Synechocystis sp. PCC 6803

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.B43 25
-
 assay at Cereibacter sphaeroides
2.5.1.62 25
-
 assay at Synechocystis sp. PCC 6803

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.B43 8
-
 assay at Cereibacter sphaeroides
2.5.1.62 8
-
 assay at Synechocystis sp. PCC 6803

General Information

EC Number General Information Comment Organism
2.5.1.B43 metabolism bacteriochlorophyll and chlorophyll share a common biosynthetic pathway up to the precursor chlorophyllide Cereibacter sphaeroides