BRENDA - Enzyme Database show

Redesigning human 2'-deoxycytidine kinase enantioselectivity for L-nucleoside analogues as reporters in positron emission tomography

Muthu, P.; Chen, H.X.; Lutz, S.; ACS Chem. Biol. 9, 2326-2333 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.74
expressed in Escherichia coli BL21(DE3)pLysS cells
Homo sapiens
Crystallization (Commentary)
EC Number
Crystallization
Organism
2.7.1.74
in complex with 5-methyldeoxycytidine and ADP, hanging drop vapor diffusion method
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.74
A100V/R104M/D133S
the mutant shows exquisitely high activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
A100V/R104M/D133S/E196A
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
A100V/R104M/D133S/E196L
inactive
Homo sapiens
2.7.1.74
A100V/R104M/D133S/F96D
inactive
Homo sapiens
2.7.1.74
A100V/R104M/D133S/F96Y
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
A100V/R104M/D133S/W58E
inactive
Homo sapiens
2.7.1.74
A100V/R104M/D133S/W58V
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/A138I
inactive
Homo sapiens
2.7.1.74
R104M/D133N/L102Y
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/L191A
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/M85Y
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/P89F
inactive
Homo sapiens
2.7.1.74
R104M/D133N/V130T
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/V130T/L191A
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/V55E
the mutant shows very low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/V55F
the mutant shows very low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/V55F/V130T
the mutant shows very low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/V55F/V130T/L191A
the mutant shows very low activity for D-deoxycytidine and D-thymidine
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
P27707
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.74
Ni-NTA agarose resin column chromatography
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + deoxycytidine
-
737326
Homo sapiens
ADP + dCMP
-
-
-
?
2.7.1.74
ATP + thymidine
-
737326
Homo sapiens
ADP + L-dTMP
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
expressed in Escherichia coli BL21(DE3)pLysS cells
Homo sapiens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.7.1.74
in complex with 5-methyldeoxycytidine and ADP, hanging drop vapor diffusion method
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.74
A100V/R104M/D133S
the mutant shows exquisitely high activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
A100V/R104M/D133S/E196A
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
A100V/R104M/D133S/E196L
inactive
Homo sapiens
2.7.1.74
A100V/R104M/D133S/F96D
inactive
Homo sapiens
2.7.1.74
A100V/R104M/D133S/F96Y
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
A100V/R104M/D133S/W58E
inactive
Homo sapiens
2.7.1.74
A100V/R104M/D133S/W58V
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/A138I
inactive
Homo sapiens
2.7.1.74
R104M/D133N/L102Y
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/L191A
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/M85Y
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/P89F
inactive
Homo sapiens
2.7.1.74
R104M/D133N/V130T
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/V130T/L191A
the mutant shows low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/V55E
the mutant shows very low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/V55F
the mutant shows very low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/V55F/V130T
the mutant shows very low activity for D-deoxycytidine and D-thymidine
Homo sapiens
2.7.1.74
R104M/D133N/V55F/V130T/L191A
the mutant shows very low activity for D-deoxycytidine and D-thymidine
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
Ni-NTA agarose resin column chromatography
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + deoxycytidine
-
737326
Homo sapiens
ADP + dCMP
-
-
-
?
2.7.1.74
ATP + thymidine
-
737326
Homo sapiens
ADP + L-dTMP
-
-
-
?