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Literature summary extracted from

  • Pan, J.J.; Solbiati, J.O.; Ramamoorthy, G.; Hillerich, B.S.; Seidel, R.D.; Cronan, J.E.; Almo, S.C.; Poulter, C.D.
    Biosynthesis of squalene from farnesyl diphosphate in bacteria: three steps catalyzed by three enzymes (2015), ACS Cent. Sci., 1, 77-82.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.8.1 expression in Escherichia coli Rhodopseudomonas palustris
1.17.8.1 expression in Escherichia coli Zymomonas mobilis
4.2.3.156 expression in Escherichia coli Zymomonas mobilis
4.2.3.156 expression in Escherichia coli Rhodopseudomonas palustris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.156 presqualene diphosphate + H2O Zymomonas mobilis
-
 hydroxysqualene + diphosphate
-
 ?
4.2.3.156 presqualene diphosphate + H2O Rhodopseudomonas palustris
-
 hydroxysqualene + diphosphate
-
 ?
4.2.3.156 presqualene diphosphate + H2O Rhodopseudomonas palustris ATCC BAA-98
-
 hydroxysqualene + diphosphate
-
 ?
4.2.3.156 presqualene diphosphate + H2O Zymomonas mobilis ZM4
-
 hydroxysqualene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.8.1 Rhodopseudomonas palustris
-
-
-
1.17.8.1 Zymomonas mobilis Q56996
-
-
4.2.3.156 Rhodopseudomonas palustris Q6N3F1
-
-
4.2.3.156 Rhodopseudomonas palustris ATCC BAA-98 Q6N3F1
-
-
4.2.3.156 Zymomonas mobilis Q5NP67
-
-
4.2.3.156 Zymomonas mobilis ZM4 Q5NP67
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.8.1 hydroxysqualene + FADH2
-
 Rhodopseudomonas palustris squalene + FAD + H2O
-
 ?
1.17.8.1 hydroxysqualene + FADH2
-
 Zymomonas mobilis squalene + FAD + H2O
-
 ?
4.2.3.156 additional information no substrate: farnesyl diphosphate Zymomonas mobilis ?
-
 ?
4.2.3.156 additional information no substrate: farnesyl diphosphate Rhodopseudomonas palustris ?
-
 ?
4.2.3.156 additional information no substrate: farnesyl diphosphate Rhodopseudomonas palustris ATCC BAA-98 ?
-
 ?
4.2.3.156 additional information no substrate: farnesyl diphosphate Zymomonas mobilis ZM4 ?
-
 ?
4.2.3.156 presqualene diphosphate + H2O
-
 Zymomonas mobilis hydroxysqualene + diphosphate
-
 ?
4.2.3.156 presqualene diphosphate + H2O
-
 Rhodopseudomonas palustris hydroxysqualene + diphosphate
-
 ?
4.2.3.156 presqualene diphosphate + H2O
-
 Rhodopseudomonas palustris ATCC BAA-98 hydroxysqualene + diphosphate
-
 ?
4.2.3.156 presqualene diphosphate + H2O
-
 Zymomonas mobilis ZM4 hydroxysqualene + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.8.1 hpnE
-
 Rhodopseudomonas palustris
1.17.8.1 hpnE
-
 Zymomonas mobilis
4.2.3.156 hpnC
-
 Zymomonas mobilis
4.2.3.156 hpnC
-
 Rhodopseudomonas palustris
4.2.3.156 RPA3743
-
 Rhodopseudomonas palustris
4.2.3.156 ZMO0869
-
 Zymomonas mobilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.8.1 FAD
-
 Rhodopseudomonas palustris
1.17.8.1 FAD
-
 Zymomonas mobilis

General Information

EC Number General Information Comment Organism
1.17.8.1 physiological function the reactions catalyzed by HpnC-HpnE constitute a three-step route for biosynthesis of squalene from farnesyl diphosphate. HpnD catalyzes formation of presqualene diphosphate from two molecules of farnesyl diphosphate, HpnC converts presqualene diphosphate to hydroxysqualene, and HpnE, a member of the amine oxidoreductase family, reduces hydroxysqualene to squalene Rhodopseudomonas palustris
1.17.8.1 physiological function the reactions catalyzed by HpnC-HpnE constitute a three-step route for biosynthesis of squalene from farnesyl diphosphate. HpnD catalyzes formation of presqualene diphosphate from two molecules of farnesyl diphosphate, HpnC converts presqualene diphosphate to hydroxysqualene, and HpnE, a member of the amine oxidoreductase family, reduces hydroxysqualene to squalene Zymomonas mobilis
4.2.3.156 physiological function the gene products of HpnC, HpnD, HpnE, and HpnF are responsible for conversion of farnesyl diphosphate to hopene or related pentacyclic metabolites. HpnD catalyzes formation of presqualene diphosphate from two molecules of farnesyl diphosphate. HpnC converts presqualene diphosphate to hydroxysqualene, and HpnE, a member of the amine oxidoreductase family, reduces hydroxysqualene to squalene Rhodopseudomonas palustris