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Literature summary extracted from
- beta-Cyclodextrin production by the cyclodextrin glucanotransferase from Paenibacillus illinoisensis ZY-08: Cloning, purification, and properties (2013), World J. Microbiol. Biotechnol., 29, 865-873.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.19 | expressed in Escherichia coli BL21(DE3) cells | Paenibacillus illinoisensis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.19 | Ba2+ | 68% residual activity at 10 mM | Paenibacillus illinoisensis |  |
| 2.4.1.19 | Cu2+ | 25% residual activity at 10 mM | Paenibacillus illinoisensis | |
| 2.4.1.19 | Hg2+ | complete inhibition at 1 mM | Paenibacillus illinoisensis | |
| 2.4.1.19 | Mg2+ | 89% residual activity at 10 mM | Paenibacillus illinoisensis | |
| 2.4.1.19 | additional information | not inhibited by EDTA | Paenibacillus illinoisensis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.19 | Ca2+ | slight activating effect (113% activity at 10 mM) | Paenibacillus illinoisensis | |
| 2.4.1.19 | additional information | Ni2+ and Na+ ions have weak effects on the enzyme activity | Paenibacillus illinoisensis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 74000 | - |
x * 74000, SDS-PAGE | Paenibacillus illinoisensis |
| 2.4.1.19 | 74352 | - |
x * 74352, calculated from amino acid sequence | Paenibacillus illinoisensis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.19 | soluble starch + glycosyl acceptor | Paenibacillus illinoisensis | - |
beta-cyclodextrin | - |
? | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | Paenibacillus illinoisensis ZY-08 | - |
beta-cyclodextrin | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.19 | Paenibacillus illinoisensis | - |
- |
- |
| 2.4.1.19 | Paenibacillus illinoisensis ZY-08 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.19 | HisTrap column chromatography | Paenibacillus illinoisensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.19 | additional information | the native enzyme does not convert king oyster mushroom powder and enoki mushroom powder, while recombinant enzyme converts king oyster mushroom powder and enoki mushroom powder to beta-cyclodextrin | Paenibacillus illinoisensis | ? | - |
? | |
| 2.4.1.19 | additional information | the native enzyme does not convert king oyster mushroom powder and enoki mushroom powder, while recombinant enzyme converts king oyster mushroom powder and enoki mushroom powder to beta-cyclodextrin | Paenibacillus illinoisensis ZY-08 | ? | - |
? | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | - |
Paenibacillus illinoisensis | beta-cyclodextrin | - |
? | |
| 2.4.1.19 | soluble starch + glycosyl acceptor | - |
Paenibacillus illinoisensis ZY-08 | beta-cyclodextrin | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.19 | ? | x * 74000, SDS-PAGE | Paenibacillus illinoisensis |
| 2.4.1.19 | ? | x * 74352, calculated from amino acid sequence | Paenibacillus illinoisensis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.4.1.19 | CGTase | - |
Paenibacillus illinoisensis |
| 2.4.1.19 | cyclodextrin glucanotransferase | - |
Paenibacillus illinoisensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 40 | - |
- |
Paenibacillus illinoisensis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 20 | 60 | about 65% activity at 20°C, around 95-100% activity at 30-50°C, about 50% activity at 60°C | Paenibacillus illinoisensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 20 | 60 | the enzyme remains fully stable between 20 and 40°C for 30 min, while at 50°C activity decreases to about 65% and is lost at 60°C | Paenibacillus illinoisensis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 7.4 | - |
- |
Paenibacillus illinoisensis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 5 | 9 | more than 60% activity between pH 5.0 and 9.0 | Paenibacillus illinoisensis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 6 | 9 | the enzyme is stable from pH 6.0 to 9.0 for 30 min with a gradual loss of activity at higher and lower pH values in the range of 6.0-9.0 | Paenibacillus illinoisensis |
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