EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.3.3 | gene argK, overexpression of His-tagged enzyme | Pseudomonas savastanoi pv. phaseolicola |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.3.3 | additional information | generation of in-frame deletion mutants of ArgK | Pseudomonas savastanoi pv. phaseolicola |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.3.3 | additional information | Ndelta(N'-sulfo-diaminophosphinyl)-ornithyl-alanyl-homoarginine and Ndelta (N'-sulfo-diaminophosphinyl)-ornithine are irreversible inhibitor of ornithine transcarbamylase attributed to the chemical structure similarity with tetrahedral intermediates in OTCase mechanism. But the enzyme from Pseudomona syringae pv. phaseolicola shows resistance against the phytotoxic compounds.Inhibition of OTCase blocks the biosynthesis of arginine in vivo and leads to a reduction of protein synthesis | Pseudomonas savastanoi pv. phaseolicola |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.3.3 | carbamoyl phosphate + L-Orn-Ala-hArg | Pseudomonas savastanoi pv. phaseolicola | the enzyme produces Cit-Ala-hArg from tripeptide Orn-Ala-hArg and carbamyl phosphate as substrates | phosphate + L-Cit-Ala-hArg | - |
? | |
2.1.3.3 | carbamoyl phosphate + L-Orn-Ala-hArg | Pseudomonas savastanoi pv. phaseolicola 1448A | the enzyme produces Cit-Ala-hArg from tripeptide Orn-Ala-hArg and carbamyl phosphate as substrates | phosphate + L-Cit-Ala-hArg | - |
? | |
2.1.3.3 | carbamoyl phosphate + L-ornithine | Pseudomonas savastanoi pv. phaseolicola | - |
phosphate + L-citrulline | - |
? | |
2.1.3.3 | carbamoyl phosphate + L-ornithine | Pseudomonas savastanoi pv. phaseolicola 1448A | - |
phosphate + L-citrulline | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.3.3 | Pseudomonas savastanoi pv. phaseolicola | P68747 | gene argK | - |
2.1.3.3 | Pseudomonas savastanoi pv. phaseolicola 1448A | P68747 | gene argK | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.3.3 | recombinant His-tagged enzyme | Pseudomonas savastanoi pv. phaseolicola |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.3.3 | carbamoyl phosphate + L-Orn-Ala-hArg | - |
Pseudomonas savastanoi pv. phaseolicola | phosphate + L-Cit-Ala-hArg | - |
? | |
2.1.3.3 | carbamoyl phosphate + L-Orn-Ala-hArg | the enzyme produces Cit-Ala-hArg from tripeptide Orn-Ala-hArg and carbamyl phosphate as substrates | Pseudomonas savastanoi pv. phaseolicola | phosphate + L-Cit-Ala-hArg | - |
? | |
2.1.3.3 | carbamoyl phosphate + L-Orn-Ala-hArg | - |
Pseudomonas savastanoi pv. phaseolicola 1448A | phosphate + L-Cit-Ala-hArg | - |
? | |
2.1.3.3 | carbamoyl phosphate + L-Orn-Ala-hArg | the enzyme produces Cit-Ala-hArg from tripeptide Orn-Ala-hArg and carbamyl phosphate as substrates | Pseudomonas savastanoi pv. phaseolicola 1448A | phosphate + L-Cit-Ala-hArg | - |
? | |
2.1.3.3 | carbamoyl phosphate + L-ornithine | - |
Pseudomonas savastanoi pv. phaseolicola | phosphate + L-citrulline | - |
? | |
2.1.3.3 | carbamoyl phosphate + L-ornithine | - |
Pseudomonas savastanoi pv. phaseolicola 1448A | phosphate + L-citrulline | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.3.3 | ArgK | - |
Pseudomonas savastanoi pv. phaseolicola |
2.1.3.3 | ornithine transcarbamylase | - |
Pseudomonas savastanoi pv. phaseolicola |
2.1.3.3 | otcase | - |
Pseudomonas savastanoi pv. phaseolicola |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.3.3 | 28 | - |
assay at | Pseudomonas savastanoi pv. phaseolicola |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.3.3 | 8 | - |
assay at | Pseudomonas savastanoi pv. phaseolicola |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.3.3 | physiological function | the enzyme ArgK in Pseudomona syringae pv. phaseolicola is resonsible for self-defense in the antimetabolic phytotoxin phaseolotoxin, a sulfodiaminophosphinyl tripeptide produced to inhibit plant host cell's ornithine transcarbamylase activity and induce arginine auxotrophic phenotype. Phaseolotoxin-resistant OTCase ArgK acts as a functional replacement of housekeeping OTCase ArgF, which is the acting target of phaseolotoxin, to confer phaseolotoxin producers with self-resistance. But neither transcriptional regulator nor thermal regulation related protein encoding gene is detected from PHT biosynthetic gene cluster | Pseudomonas savastanoi pv. phaseolicola |