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Literature summary extracted from

  • Angelaccio, S.; Dworkowski, F.; Di Bello, A.; Milano, T.; Capitani, G.; Pascarella, S.
    Conformational transitions driven by pyridoxal-5-phosphate uptake in the psychrophilic serine hydroxymethyltransferase from Psychromonas ingrahamii (2014), Proteins, 82, 2831-2841.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 gene glyA, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain HMS174 (DE3) Psychromonas ingrahamii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.1 purified recombinant enzyme in apoform, mixing of 200 nl of 10 mg/ml protein in 50 mM HEPES, pH 7.2, and 0.2 mM DTT with 200 nl of well solution containing 2M ammonium sulfate, 150 mM sodium chloride, and 100 mM sodium cacodylate, pH 6.5, 4°C, several days, X-ray diffraction structure determination and analysis at 1.85 A resolution, molecular replacement and modeling Psychromonas ingrahamii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 45050
-
 2 * 45050, recombinant N-terminally His-tagged enzyme, mass spectrometry Psychromonas ingrahamii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Psychromonas ingrahamii
-
 tetrahydrofolate + L-serine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Psychromonas ingrahamii A1SUU0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 recombinant N-terminally His-tagged enzyme from Escherichia coli strain HMS174 (DE3) by nickel affinity chromatography Psychromonas ingrahamii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Psychromonas ingrahamii tetrahydrofolate + L-serine
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.2.1 dimer 2 * 45050, recombinant N-terminally His-tagged enzyme, mass spectrometry Psychromonas ingrahamii

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Psychromonas ingrahamii
2.1.2.1 SHMT
-
 Psychromonas ingrahamii

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 5,10-methylenetetrahydrofolate
-
 Psychromonas ingrahamii
2.1.2.1 pyridoxal 5'-phosphate dependent on, cofactor binding triggers a rearrangement of the small domain that moves toward the large domain and screens the pyridoxal 5'-phosphate binding site at the solvent side Psychromonas ingrahamii
2.1.2.1 tetrahydrofolate
-
 Psychromonas ingrahamii

General Information

EC Number General Information Comment Organism
2.1.2.1 evolution the enzyme belongs to the fold type-I superfamily of PLP-dependent enzymes Psychromonas ingrahamii
2.1.2.1 additional information the enzyme psychrophilic shows high catalytic activity at low temperature and thermolability, three-dimensional structure analysis and structure-function relationship, homology modeling of the holoenzyme form, overview. The apoform enzyme is in an open conformation and possesses four or five (in chain A) disordered loops that interact with the cofactor. Cofactor binding triggers a rearrangement of the small domain that moves toward the large domain and screens the pyridoxal 5'-phosphate binding site at the solvent side Psychromonas ingrahamii
2.1.2.1 physiological function SHMTs are an important group of pyridoxal-5'-phosphate-dependent enzymes that catalyze the reversible conversion of L-serine and tetrahydropteroylglutamate to glycine and 5,10-methylenetetrahydropteroylglutamate. The enzyme plays a central role in one-carbon unit metabolism Psychromonas ingrahamii