EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.2.13 | expressed in Escherichia coli BL21(DE3) cells | Streptomyces mobaraensis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.2.13 | D20A | the mutant shows reduced activity compared to the wild type enzyme | Streptomyces mobaraensis |
2.3.2.13 | E29A | the mutant shows about 60% reduced activity compared to the wild type enzyme | Streptomyces mobaraensis |
2.3.2.13 | I24A | the mutant shows reduced activity compared to the wild type enzyme | Streptomyces mobaraensis |
2.3.2.13 | L16A | the mutant shows wild type activity | Streptomyces mobaraensis |
2.3.2.13 | L27A | the mutant shows about 40% reduced activity compared to the wild type enzyme | Streptomyces mobaraensis |
2.3.2.13 | N23A | the mutant shows about 50% reduced activity compared to the wild type enzyme | Streptomyces mobaraensis |
2.3.2.13 | N25A | the mutant shows reduced activity compared to the wild type enzyme | Streptomyces mobaraensis |
2.3.2.13 | N28A | the mutant shows about 45% reduced activity compared to the wild type enzyme | Streptomyces mobaraensis |
2.3.2.13 | V21A | the mutant shows about wild type activity | Streptomyces mobaraensis |
2.3.2.13 | Y10A | the mutant shows reduced activity compared to the wild type enzyme | Streptomyces mobaraensis |
2.3.2.13 | Y14A | the mutant shows wild type activity | Streptomyces mobaraensis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.2.13 | 39198 | - |
x * 39198, mass spectrometry | Streptomyces mobaraensis |
2.3.2.13 | 39200 | - |
x * 39200, calculated from amino acid sequence | Streptomyces mobaraensis |
2.3.2.13 | 40000 | - |
x * 40000, SDS-PAGE | Streptomyces mobaraensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.13 | [protein]-L-glutamine + alkylamine | Streptomyces mobaraensis | - |
[protein]-N5-alkyl-L-glutamine + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.2.13 | Streptomyces mobaraensis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.2.13 | Ni-NTA column chromatography and Superdex 200 gel filtration | Streptomyces mobaraensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.13 | Z-Gln-Gly + alkylamine | - |
Streptomyces mobaraensis | ? | - |
? | |
2.3.2.13 | [protein]-L-glutamine + alkylamine | - |
Streptomyces mobaraensis | [protein]-N5-alkyl-L-glutamine + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.2.13 | ? | x * 40000, SDS-PAGE | Streptomyces mobaraensis |
2.3.2.13 | ? | x * 39198, mass spectrometry | Streptomyces mobaraensis |
2.3.2.13 | ? | x * 39200, calculated from amino acid sequence | Streptomyces mobaraensis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.2.13 | microbial transglutaminase | - |
Streptomyces mobaraensis |
2.3.2.13 | MTGase | - |
Streptomyces mobaraensis |
2.3.2.13 | protein-glutamine gamma-glutamyltransferase | - |
Streptomyces mobaraensis |