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Literature summary extracted from

  • Rickert, M.; Strop, P.; Lui, V.; Melton-Witt, J.; Farias, S.E.; Foletti, D.; Shelton, D.; Pons, J.; Rajpal, A.
    Production of soluble and active microbial transglutaminase in Escherichia coli for site-specific antibody drug conjugation (2016), Protein Sci., 25, 442-455.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.13 expressed in Escherichia coli BL21(DE3) cells Streptomyces mobaraensis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.13 D20A the mutant shows reduced activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 E29A the mutant shows about 60% reduced activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 I24A the mutant shows reduced activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 L16A the mutant shows wild type activity Streptomyces mobaraensis
2.3.2.13 L27A the mutant shows about 40% reduced activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 N23A the mutant shows about 50% reduced activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 N25A the mutant shows reduced activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 N28A the mutant shows about 45% reduced activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 V21A the mutant shows about wild type activity Streptomyces mobaraensis
2.3.2.13 Y10A the mutant shows reduced activity compared to the wild type enzyme Streptomyces mobaraensis
2.3.2.13 Y14A the mutant shows wild type activity Streptomyces mobaraensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.13 39198
-
x * 39198, mass spectrometry Streptomyces mobaraensis
2.3.2.13 39200
-
x * 39200, calculated from amino acid sequence Streptomyces mobaraensis
2.3.2.13 40000
-
x * 40000, SDS-PAGE Streptomyces mobaraensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.13 [protein]-L-glutamine + alkylamine Streptomyces mobaraensis
-
[protein]-N5-alkyl-L-glutamine + NH3
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Streptomyces mobaraensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 Ni-NTA column chromatography and Superdex 200 gel filtration Streptomyces mobaraensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 Z-Gln-Gly + alkylamine
-
Streptomyces mobaraensis ?
-
?
2.3.2.13 [protein]-L-glutamine + alkylamine
-
Streptomyces mobaraensis [protein]-N5-alkyl-L-glutamine + NH3
-
?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 ? x * 40000, SDS-PAGE Streptomyces mobaraensis
2.3.2.13 ? x * 39198, mass spectrometry Streptomyces mobaraensis
2.3.2.13 ? x * 39200, calculated from amino acid sequence Streptomyces mobaraensis

Synonyms

EC Number Synonyms Comment Organism
2.3.2.13 microbial transglutaminase
-
Streptomyces mobaraensis
2.3.2.13 MTGase
-
Streptomyces mobaraensis
2.3.2.13 protein-glutamine gamma-glutamyltransferase
-
Streptomyces mobaraensis