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Literature summary extracted from

  • Okano, H.; Kanaya, E.; Ozaki, M.; Angkawidjaja, C.; Kanaya, S.
    Structure, activity, and stability of metagenome-derived glycoside hydrolase family 9 endoglucanase with an N-terminal Ig-like domain (2015), Protein Sci., 24, 408-419.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Escherichia coli uncultured bacterium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.4 to 2.15 A resolution. Residues Asp194 and Asp197 form hydrogen bonds with a water molecule that may function as a nucleophile uncultured bacterium

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.4 D99A 106% of wild-type activity, temperature of the midpoint of the thermal denaturation transition is decreased by 1.9°C uncultured bacterium
3.2.1.4 additional information a mutant lacking the immunoglobulin-like domain shows 1% of wild-type activity and a decrease in the temperature of the midpoint of the thermal denaturation transition by 6.3°C uncultured bacterium
3.2.1.4 Q40A 94% of wild-type activity uncultured bacterium
3.2.1.4 Q40A/D99A 97% of wild-type activity, temperature of the midpoint of the thermal denaturation transition is decreased by 5.0°C uncultured bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 60000
-
 gel filtration uncultured bacterium
3.2.1.4 62600
-
 1 * 62600, calculated uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 uncultured bacterium W8PF21
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 34.4
-
 wild-type, pH 7.0, 50°C uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.4 additional information no substrate: 4-nitrophenyl cellobioside uncultured bacterium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 monomer 1 * 62600, calculated uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 CelG
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 70
-
-
 uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 81.4
-
 denaturation temperature uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 5 9 more than 80% of maximum activity uncultured bacterium